FastQCFastQC Report
Thu 2 Feb 2017
SRR4063015_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063015_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499111
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT50000.33353100604291475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT38120.2542840390071182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20780.13861548611143537No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17420.1162022025053515No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15740.10499556070230956No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15620.10419508628780658No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15550.10372814287934648No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15030.10025942041650018No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15020.10019271421529158No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG451.2379192E-524.8901335
ACCGTCG2900.016.5525538
CGCGCAA2550.016.31345421
TACCGTC3000.016.0008017
GTCTAAT703.6959274E-416.00081
CGCGTAA1102.0199877E-716.00026710
ATACCGT3400.015.5301896
GTATTAA2500.015.3607671
GTCCTAA2950.015.1872011
GCGCAAA2850.015.15764222
CGTCGTA3200.015.0002510
TTAGTAC756.2451325E-414.9335823
CGCAAAT2800.014.856894523
GCGTAAC1205.418369E-714.66691111
TCTAAAC1757.4578566E-1114.6288163
AACCGCG2300.014.6094277
CCGTCGT3300.014.5456989
AAGACGG4100.014.4397475
GCATTCG5000.014.39975922
GATAACG1801.1823431E-1014.22245911
Produced by FastQC (version 0.11.5)