##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063015_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1499111
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.215909295575845	32.0	32.0	32.0	32.0	32.0
2	31.322042864070774	32.0	32.0	32.0	32.0	32.0
3	31.40394407085266	32.0	32.0	32.0	32.0	32.0
4	31.49298217410185	32.0	32.0	32.0	32.0	32.0
5	31.433763076916918	32.0	32.0	32.0	32.0	32.0
6	34.96302742091813	36.0	36.0	36.0	36.0	36.0
7	34.990086791438394	36.0	36.0	36.0	36.0	36.0
8	34.93089704498199	36.0	36.0	36.0	36.0	36.0
9	35.02365135070052	36.0	36.0	36.0	36.0	36.0
10	34.88554750115235	36.0	36.0	36.0	32.0	36.0
11	35.055248744089	36.0	36.0	36.0	36.0	36.0
12	34.96176467252925	36.0	36.0	36.0	36.0	36.0
13	35.0066452717644	36.0	36.0	36.0	36.0	36.0
14	34.95151526471356	36.0	36.0	36.0	32.0	36.0
15	34.92952756667118	36.0	36.0	36.0	36.0	36.0
16	34.94364393297094	36.0	36.0	36.0	36.0	36.0
17	34.91261087404468	36.0	36.0	36.0	32.0	36.0
18	34.91206254907075	36.0	36.0	36.0	32.0	36.0
19	34.90197790557204	36.0	36.0	36.0	32.0	36.0
20	34.88823242575099	36.0	36.0	36.0	32.0	36.0
21	34.88017164839695	36.0	36.0	36.0	32.0	36.0
22	34.862242355636106	36.0	36.0	36.0	32.0	36.0
23	34.80928630368265	36.0	36.0	36.0	32.0	36.0
24	34.78510997517862	36.0	36.0	36.0	32.0	36.0
25	34.77430957414094	36.0	36.0	36.0	32.0	36.0
26	34.70820839817732	36.0	36.0	36.0	32.0	36.0
27	34.69395861947514	36.0	36.0	36.0	32.0	36.0
28	34.67069416474164	36.0	36.0	36.0	32.0	36.0
29	34.626493968758815	36.0	36.0	36.0	32.0	36.0
30	34.60422677173338	36.0	36.0	36.0	32.0	36.0
31	34.59890294981492	36.0	36.0	36.0	32.0	36.0
32	34.571285248390545	36.0	36.0	36.0	32.0	36.0
33	34.537240404479725	36.0	36.0	36.0	32.0	36.0
34	34.523518271829104	36.0	36.0	36.0	32.0	36.0
35	34.48740220037075	36.0	36.0	36.0	32.0	36.0
36	34.459358913382665	36.0	36.0	36.0	32.0	36.0
37	34.453201931011115	36.0	36.0	36.0	32.0	36.0
38	34.033217019953824	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	8.0
21	26.0
22	105.0
23	357.0
24	966.0
25	2338.0
26	5014.0
27	9742.0
28	16683.0
29	26335.0
30	39996.0
31	57785.0
32	82686.0
33	131914.0
34	327715.0
35	797438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.395658836534565	18.656622322381022	12.311793631955105	26.635925209129308
2	15.314342967265265	21.075023797437282	37.56926605167996	26.041367183617492
3	17.47482341200885	25.300194581988926	29.54564405170798	27.679337954294247
4	11.949556736997286	16.59471286296536	36.75560832760772	34.70012207242964
5	13.695801973038652	37.39834795201683	33.7878699947769	15.11798008016762
6	33.16765703338252	36.83350365684127	16.84635129690456	13.152488012871654
7	28.894935451572167	31.209446690596472	21.83673346865834	18.05888438917302
8	27.306651154909883	33.757015979155184	19.88355509186313	19.052777774071796
9	27.531308015639166	13.829694267796356	18.84518067024803	39.79381704631645
10	15.41970851611113	27.18119283190847	32.35533871260393	25.043759939376475
11	36.964207807386316	21.15144205432536	23.025172076566232	18.859178061722087
12	24.336755148231582	24.005624681893952	29.645715749442836	22.01190442043163
13	29.53060784772822	19.805097561284736	25.421100133479285	25.243194457507762
14	23.199349481125815	20.109318122540625	25.787349969415207	30.903982426918354
15	24.694702393618616	27.816819434985135	22.735274439317703	24.753203732078546
16	25.042908763927418	25.91796071138161	24.579167253125352	24.459963271565616
17	23.49385735946171	26.171977925583896	25.8805385325036	24.4536261824508
18	24.457561848322108	24.991411576594395	27.131012980359692	23.420013594723805
19	25.329278485715868	25.06698970256372	26.028693005387858	23.575038806332554
20	25.415929841085816	24.52887077741408	25.698430603204166	24.356768778295937
21	26.52618785400147	24.446355206519062	24.835986127778398	24.19147081170107
22	25.21034132896097	24.886149191087252	25.573356475938073	24.330153004013713
23	23.91985911640907	24.384001174029923	26.190206189005476	25.50593352055553
24	24.54928287498391	25.26877596121968	25.83724620791923	24.344694955877184
25	24.559087510097676	24.762808792167604	26.012953044712617	24.6651506530221
26	24.108288178793966	25.580694158071015	26.417256627427854	23.89376103570716
27	24.835985690147535	25.151973808407273	25.48844078508072	24.523599716364476
28	24.003759561500114	25.06598910954559	26.227610897391855	24.70264043156244
29	24.122963543059853	25.056049885565514	26.04937192776252	24.771614643612114
30	24.0603931263262	25.26930961082935	26.32913773563132	24.341159527213126
31	24.721051343096008	24.939647564456536	25.358962745253688	24.98033834719377
32	24.218019879782084	25.31126781138955	25.407324741129912	25.06338756769846
33	23.95126178114896	24.769746869978274	26.177781365089043	25.101209983783722
34	24.642137907066257	24.863802613682378	26.295918047429446	24.198141431821927
35	25.24542879079668	24.924038313373725	26.002010524904428	23.828522370925167
36	24.224156850293273	25.51692302971561	25.65020201973036	24.608718100260756
37	24.91896864208187	25.33301403298355	25.45608697421338	24.291930350721195
38	24.226673461818628	25.21192602534307	25.947897016092234	24.613503496746063
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	264.0
1	252.0
2	240.0
3	240.0
4	808.5
5	1377.0
6	1377.0
7	1550.5
8	1724.0
9	1642.0
10	1560.0
11	1560.0
12	2054.0
13	2548.0
14	3344.0
15	4140.0
16	4140.0
17	6394.0
18	8648.0
19	8648.0
20	10214.0
21	11780.0
22	12739.0
23	13698.0
24	13698.0
25	16107.5
26	18517.0
27	18517.0
28	23442.5
29	28368.0
30	34428.5
31	40489.0
32	40489.0
33	50673.5
34	60858.0
35	60858.0
36	66507.0
37	72156.0
38	80570.0
39	88984.0
40	88984.0
41	93019.0
42	97054.0
43	107447.0
44	117840.0
45	117840.0
46	119967.5
47	122095.0
48	122095.0
49	129235.5
50	136376.0
51	135885.5
52	135395.0
53	135395.0
54	128882.0
55	122369.0
56	122369.0
57	118220.5
58	114072.0
59	101681.5
60	89291.0
61	89291.0
62	83656.0
63	78021.0
64	63901.0
65	49781.0
66	49781.0
67	41514.0
68	33247.0
69	33247.0
70	26781.5
71	20316.0
72	15688.0
73	11060.0
74	11060.0
75	8218.0
76	5376.0
77	5376.0
78	5199.5
79	5023.0
80	3867.5
81	2712.0
82	2712.0
83	2577.5
84	2443.0
85	2443.0
86	1544.5
87	646.0
88	569.0
89	492.0
90	492.0
91	285.5
92	79.0
93	59.5
94	40.0
95	40.0
96	26.0
97	12.0
98	12.0
99	16.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010539579790956106
2	0.0
3	0.0
4	6.670620120858295E-5
5	1.334124024171659E-4
6	2.0011860362574886E-4
7	2.668248048343318E-4
8	0.0022013046398832374
9	0.0036021348652634797
10	0.0015342426277974081
11	0.004602727883392224
12	2.668248048343318E-4
13	1.334124024171659E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	6.670620120858295E-5
24	0.0
25	2.668248048343318E-4
26	0.0
27	2.668248048343318E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.0011860362574886E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1499111.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.24721718526536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.63796798873541	44.794140816556116
2	12.814338697312747	14.415417835866009
3	3.5034117608381607	5.911714866038309
4	1.3770357650694351	3.098177189989543
5	0.7132384781761248	2.005883979343032
6	0.42401102612788577	1.430966416533745
7	0.26871092003308406	1.0579969035407346
8	0.20581980971714917	0.9261433230552383
9	0.14495182964380257	0.7337823339038895
>10	0.7535921645327568	7.567903818914427
>50	0.06835336025209685	2.697155430876126
>100	0.07579634485610143	8.864267557825793
>500	0.00954905024701337	3.6070797143250974
>1k	0.003222804458367012	2.8893698132318595
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5000	0.33353100604291475	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3812	0.2542840390071182	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2078	0.13861548611143537	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1742	0.1162022025053515	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1574	0.10499556070230956	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1562	0.10419508628780658	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1555	0.10372814287934648	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1503	0.10025942041650018	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1502	0.10019271421529158	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.670620120858295E-5
7	0.0	0.0	0.0	0.0	6.670620120858295E-5
8	0.0	0.0	0.0	0.0	6.670620120858295E-5
9	0.0	0.0	0.0	0.0	6.670620120858295E-5
10	0.0	0.0	0.0	0.0	1.334124024171659E-4
11	0.0	0.0	0.0	6.670620120858295E-5	1.334124024171659E-4
12	0.0	0.0	0.0	6.670620120858295E-5	2.0011860362574886E-4
13	0.0	0.0	0.0	6.670620120858295E-5	2.0011860362574886E-4
14	0.0	0.0	0.0	6.670620120858295E-5	2.0011860362574886E-4
15	0.0	0.0	0.0	1.334124024171659E-4	2.668248048343318E-4
16	0.0	0.0	0.0	2.0011860362574886E-4	2.668248048343318E-4
17	0.0	0.0	0.0	2.668248048343318E-4	2.668248048343318E-4
18	0.0	0.0	0.0	3.335310060429148E-4	2.668248048343318E-4
19	0.0	0.0	0.0	3.335310060429148E-4	2.668248048343318E-4
20	0.0	0.0	0.0	4.002372072514977E-4	4.002372072514977E-4
21	0.0	0.0	0.0	4.002372072514977E-4	4.669434084600807E-4
22	0.0	0.0	0.0	6.003558108772465E-4	4.669434084600807E-4
23	0.0	0.0	0.0	7.337682132944125E-4	4.669434084600807E-4
24	0.0	0.0	0.0	0.001534242627797408	4.669434084600807E-4
25	0.0	0.0	0.0	0.0022680108410918203	4.669434084600807E-4
26	0.0	0.0	0.0	0.00293507285317765	5.336496096686636E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	45	1.2379192E-5	24.890133	5
ACCGTCG	290	0.0	16.552553	8
CGCGCAA	255	0.0	16.313454	21
TACCGTC	300	0.0	16.000801	7
GTCTAAT	70	3.6959274E-4	16.0008	1
CGCGTAA	110	2.0199877E-7	16.000267	10
ATACCGT	340	0.0	15.530189	6
GTATTAA	250	0.0	15.360767	1
GTCCTAA	295	0.0	15.187201	1
GCGCAAA	285	0.0	15.157642	22
CGTCGTA	320	0.0	15.00025	10
TTAGTAC	75	6.2451325E-4	14.933582	3
CGCAAAT	280	0.0	14.8568945	23
GCGTAAC	120	5.418369E-7	14.666911	11
TCTAAAC	175	7.4578566E-11	14.628816	3
AACCGCG	230	0.0	14.609427	7
CCGTCGT	330	0.0	14.545698	9
AAGACGG	410	0.0	14.439747	5
GCATTCG	500	0.0	14.399759	22
GATAACG	180	1.1823431E-10	14.222459	11
>>END_MODULE