FastQCFastQC Report
Thu 2 Feb 2017
SRR4063014_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063014_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736447
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29500.4005719352512809No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21170.28746128370405477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11590.15737724506990997No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9370.1272325096035424No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.12383783218615867No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8780.1192210708985168No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC200.00372395332.0501981
ATCGTTT1400.020.57011829
CGTCTTA400.004481864620.00008615
GTCTAAA507.117109E-419.2301181
TCTCGTC507.195659E-419.19877812
CATCGTT1500.019.19877628
CGTTTAT1550.018.57946231
GATATAC2550.018.224621
ACGCTTC1850.018.16100731
TCGTTTA1600.017.99885430
AACGAAT1352.3646862E-1117.77664631
GTCTTAG550.0013497617.4819261
ACCCGTT550.001364596817.45343430
CGCTTCG1900.016.84103232
GTCCTAC5700.016.0250991
CATAGGG908.549989E-616.0044142
ACGTATG805.6195167E-515.99898132
TATAGTG1102.0192965E-715.9978945
TTTAGAC805.6260487E-515.996813
ATATACT1102.0208608E-715.9968094
Produced by FastQC (version 0.11.5)