##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063014_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	736447
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17878408086393	32.0	32.0	32.0	32.0	32.0
2	30.80634180056406	32.0	32.0	32.0	32.0	32.0
3	30.865851853561765	32.0	32.0	32.0	32.0	32.0
4	30.92227275010965	32.0	32.0	32.0	32.0	32.0
5	30.856349472535022	32.0	32.0	32.0	32.0	32.0
6	34.49470634003533	36.0	36.0	36.0	32.0	36.0
7	34.40890383150451	36.0	36.0	36.0	32.0	36.0
8	34.39368888731979	36.0	36.0	36.0	32.0	36.0
9	34.51431535466911	36.0	36.0	36.0	32.0	36.0
10	34.24489610250296	36.0	36.0	36.0	32.0	36.0
11	34.53665233207549	36.0	36.0	36.0	32.0	36.0
12	34.344912804315854	36.0	36.0	36.0	32.0	36.0
13	34.42701783020367	36.0	36.0	36.0	32.0	36.0
14	34.33981128309301	36.0	36.0	36.0	32.0	36.0
15	34.28173106822351	36.0	36.0	36.0	32.0	36.0
16	34.29905207027797	36.0	36.0	36.0	32.0	36.0
17	34.24064732424737	36.0	36.0	36.0	32.0	36.0
18	34.25904783372055	36.0	36.0	36.0	32.0	36.0
19	34.23684121192699	36.0	36.0	36.0	32.0	36.0
20	34.21308254361821	36.0	36.0	36.0	32.0	36.0
21	34.19371930362945	36.0	36.0	36.0	32.0	36.0
22	34.156709172554166	36.0	36.0	36.0	32.0	36.0
23	34.121543030252006	36.0	36.0	36.0	32.0	36.0
24	34.109506862000934	36.0	36.0	36.0	32.0	36.0
25	34.0849035979507	36.0	36.0	36.0	32.0	36.0
26	34.04285440771705	36.0	36.0	36.0	32.0	36.0
27	34.047420927778916	36.0	36.0	36.0	32.0	36.0
28	34.02660205011359	36.0	36.0	36.0	32.0	36.0
29	33.98327917691294	36.0	36.0	36.0	32.0	36.0
30	33.94866568809432	36.0	36.0	36.0	32.0	36.0
31	33.97243793511278	36.0	36.0	36.0	32.0	36.0
32	33.90487706515201	36.0	36.0	36.0	32.0	36.0
33	33.87570456529798	36.0	36.0	36.0	32.0	36.0
34	33.87713440342618	36.0	36.0	36.0	32.0	36.0
35	33.83389707609645	36.0	36.0	36.0	32.0	36.0
36	33.78544552425361	36.0	36.0	36.0	32.0	36.0
37	33.76105137233229	36.0	36.0	36.0	27.0	36.0
38	33.1738957453829	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	5.0
9	1.0
10	6.0
11	3.0
12	2.0
13	2.0
14	174.0
15	314.0
16	312.0
17	419.0
18	521.0
19	642.0
20	770.0
21	1097.0
22	1629.0
23	2259.0
24	3439.0
25	5033.0
26	7263.0
27	10379.0
28	14361.0
29	19729.0
30	26827.0
31	35384.0
32	48134.0
33	72093.0
34	160594.0
35	325052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.25168070368633	17.73123695712761	11.667788268722257	25.349294070463802
2	15.646780701873563	20.27417360705406	39.58208225208212	24.496963438990257
3	18.846352327665816	24.972703568906514	29.013118583301644	27.167825520126026
4	12.04273653993175	15.93834488246501	36.824280742349124	35.19463783525411
5	13.612440321327895	37.61847472448686	34.16159273481758	14.607492219367666
6	32.705721963546395	36.4149024432305	17.632865001093094	13.24651059213001
7	29.058206575906446	31.23406196788359	21.501644383973844	18.20608707223612
8	27.317697795617306	34.36849066185796	19.83604954633223	18.477761996192505
9	27.677521501915958	14.309399467170708	19.063176390393867	38.94990264051947
10	15.828559246429789	27.488828025598792	32.34352785898666	24.339084868984752
11	36.641857825105134	21.233761207770993	23.278216153312396	18.846164813811477
12	24.683778928372107	24.25726239118125	29.975381264504076	21.083577415942568
13	29.985319421414086	20.337558243599823	25.3601902363967	24.31693209858939
14	22.93250695626077	20.893225980360867	26.024359474026564	30.149907589351795
15	24.248791220731245	29.044249470310206	23.156407888303363	23.550551420655186
16	24.002895236439784	26.294776352490963	26.028610150709824	23.673718260359433
17	22.764572145668488	26.759943610094773	26.660799508897163	23.814684735339576
18	23.581436528909013	25.227793062054772	28.632942882671976	22.557827526364246
19	24.91091652770007	25.36013623252959	26.432261253578275	23.296685986192063
20	25.053332844477218	24.991139482871706	26.855175017279702	23.100352655371374
21	26.026664349129703	24.691002615376675	25.255902250636193	24.026430784857432
22	24.93155930711946	25.892444907958133	25.937664651468488	23.23833113345392
23	23.208417301748536	25.561253035084764	26.75994176982786	24.470387893338838
24	24.136512096911094	25.909186590524826	26.674996095580195	23.279305216983886
25	24.06998978727103	25.455091154038374	26.704656569826817	23.77026248886378
26	22.892407315357307	26.71930725206909	27.363325972343322	23.02495946023028
27	23.950363797837582	26.087794915249635	26.398224872962718	23.563616413950065
28	22.976121932407032	26.155066213294802	26.788139183713376	24.080672670584786
29	23.37986319433222	25.937534374562894	26.498391440207612	24.184210990897277
30	23.007816106937813	26.12719777086151	27.09185419348531	23.773131928715365
31	24.135130877823826	25.79249115983987	26.04411660863756	24.028261353698745
32	23.28429308991556	26.60421889131208	25.956067474015555	24.155420544756804
33	22.900636890777985	26.00415540678173	27.038118388354	24.057089314086287
34	23.722417096816688	26.269910140404768	27.000655919366736	23.00701684341181
35	24.138684991410276	26.196551888041608	26.84312380745439	22.821639313093726
36	22.906840463427848	26.852577413392474	26.652262069508748	23.58832005367093
37	23.793499604826817	26.630734424608832	25.989902035073996	23.58586393549035
38	23.287905617538105	26.965350729486914	26.201100476909538	23.545643176065443
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	835.0
1	581.0
2	327.0
3	327.0
4	643.5
5	960.0
6	960.0
7	1130.5
8	1301.0
9	1173.0
10	1045.0
11	1045.0
12	1387.0
13	1729.0
14	2177.5
15	2626.0
16	2626.0
17	3843.5
18	5061.0
19	5061.0
20	5867.0
21	6673.0
22	7023.0
23	7373.0
24	7373.0
25	8954.0
26	10535.0
27	10535.0
28	13569.0
29	16603.0
30	20947.5
31	25292.0
32	25292.0
33	30363.0
34	35434.0
35	35434.0
36	38115.0
37	40796.0
38	44660.0
39	48524.0
40	48524.0
41	48754.0
42	48984.0
43	52543.0
44	56102.0
45	56102.0
46	58177.0
47	60252.0
48	60252.0
49	61888.0
50	63524.0
51	62896.5
52	62269.0
53	62269.0
54	58743.0
55	55217.0
56	55217.0
57	53152.0
58	51087.0
59	45393.0
60	39699.0
61	39699.0
62	37219.5
63	34740.0
64	28429.0
65	22118.0
66	22118.0
67	18364.5
68	14611.0
69	14611.0
70	11781.0
71	8951.0
72	6930.0
73	4909.0
74	4909.0
75	3708.5
76	2508.0
77	2508.0
78	2404.0
79	2300.0
80	1807.0
81	1314.0
82	1314.0
83	1346.5
84	1379.0
85	1379.0
86	885.5
87	392.0
88	361.5
89	331.0
90	331.0
91	229.0
92	127.0
93	123.0
94	119.0
95	119.0
96	105.0
97	91.0
98	91.0
99	200.0
100	309.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12112209025225168
2	0.05662321932196072
3	0.0118134774124954
4	0.003802038707469784
5	4.073612900860483E-4
6	5.431483867813977E-4
7	1.3578709669534943E-4
8	6.789354834767471E-4
9	1.3578709669534943E-4
10	8.147225801720966E-4
11	0.002172593547125591
12	0.002172593547125591
13	0.013578709669534943
14	0.008147225801720965
15	0.022676445148123354
16	0.008826161285197713
17	0.019689129020825667
18	0.004480974190946531
19	0.008011438705025615
20	0.00461676128764188
21	0.004480974190946531
22	0.005838845157900025
23	0.008011438705025615
24	0.01385028386292564
25	0.015072367733183788
26	0.018059683860481474
27	0.006653567738072122
28	0.005295696771118627
29	0.010048245155455857
30	0.002579954837211639
31	0.00475254838433723
32	0.006110419351290724
33	0.007739864511634917
34	0.010319819348846557
35	0.01385028386292564
36	0.014665006443097739
37	0.008283012898416315
38	0.005295696771118627
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	736447.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.42204700847232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.86216124455969	53.18709932684549
2	10.899571425417276	13.825462626300322
3	2.647643575602716	5.037569259406656
4	0.907928440977252	2.3033072105595305
5	0.4419826841140503	1.401572328440603
6	0.23844726410403605	0.9073688155828673
7	0.15273164938121536	0.6780587692715867
8	0.10957379893297595	0.5559515701459274
9	0.07461299545448022	0.42588980146412775
>10	0.5140196886915588	6.514007061053261
>50	0.07319545378489736	3.2854777729890676
>100	0.07212164213625927	8.76403422572486
>500	0.005366193610351634	2.2666309895258188
>1k	6.439432332421961E-4	0.8475702426899548
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2950	0.4005719352512809	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2117	0.28746128370405477	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1159	0.15737724506990997	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	937	0.1272325096035424	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	912	0.12383783218615867	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	878	0.1192210708985168	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.3578709669534943E-4	0.0
5	0.0	0.0	0.0	1.3578709669534943E-4	0.0
6	0.0	0.0	0.0	1.3578709669534943E-4	0.0
7	0.0	0.0	0.0	1.3578709669534943E-4	0.0
8	0.0	0.0	0.0	1.3578709669534943E-4	0.0
9	0.0	0.0	0.0	1.3578709669534943E-4	0.0
10	0.0	0.0	0.0	1.3578709669534943E-4	0.0
11	0.0	0.0	0.0	1.3578709669534943E-4	0.0
12	0.0	0.0	0.0	1.3578709669534943E-4	0.0
13	0.0	0.0	0.0	1.3578709669534943E-4	0.0
14	0.0	0.0	0.0	1.3578709669534943E-4	0.0
15	0.0	0.0	0.0	1.3578709669534943E-4	0.0
16	0.0	0.0	0.0	1.3578709669534943E-4	0.0
17	0.0	0.0	0.0	2.7157419339069887E-4	0.0
18	0.0	0.0	0.0	2.7157419339069887E-4	0.0
19	0.0	0.0	0.0	4.073612900860483E-4	0.0
20	0.0	0.0	0.0	4.073612900860483E-4	1.3578709669534943E-4
21	0.0	0.0	0.0	5.431483867813977E-4	1.3578709669534943E-4
22	0.0	0.0	0.0	9.50509676867446E-4	1.3578709669534943E-4
23	0.0	0.0	0.0	0.001901019353734892	1.3578709669534943E-4
24	0.0	0.0	0.0	0.0029873161272976874	1.3578709669534943E-4
25	0.0	0.0	0.0	0.0036662516107744346	1.3578709669534943E-4
26	0.0	0.0	0.0	0.00488833548103258	1.3578709669534943E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAC	20	0.003723953	32.050198	1
ATCGTTT	140	0.0	20.570118	29
CGTCTTA	40	0.0044818646	20.000086	15
GTCTAAA	50	7.117109E-4	19.230118	1
TCTCGTC	50	7.195659E-4	19.198778	12
CATCGTT	150	0.0	19.198776	28
CGTTTAT	155	0.0	18.579462	31
GATATAC	255	0.0	18.22462	1
ACGCTTC	185	0.0	18.161007	31
TCGTTTA	160	0.0	17.998854	30
AACGAAT	135	2.3646862E-11	17.776646	31
GTCTTAG	55	0.00134976	17.481926	1
ACCCGTT	55	0.0013645968	17.453434	30
CGCTTCG	190	0.0	16.841032	32
GTCCTAC	570	0.0	16.025099	1
CATAGGG	90	8.549989E-6	16.004414	2
ACGTATG	80	5.6195167E-5	15.998981	32
TATAGTG	110	2.0192965E-7	15.997894	5
TTTAGAC	80	5.6260487E-5	15.99681	3
ATATACT	110	2.0208608E-7	15.996809	4
>>END_MODULE