FastQCFastQC Report
Thu 2 Feb 2017
SRR4063014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736447
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT37690.511781567444772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29040.39432572880329475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17250.23423274179947776No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12320.1672897031286705No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9350.12696093541015172No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9270.12587463863658893No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8080.10971597412984234No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC7400.10048245155455857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGC555.8286936E-520.3640692
CGGTCCA2100.019.808610
CCGGTCC2400.018.6683319
GGTCCAA2550.018.19522911
GTCTAGC450.00883934117.780571
GTAATGT450.00883934117.780573
TATTGGG450.0088462617.7781542
CGAAGAT450.008849720517.77694730
TCGAACT550.001364441617.45373219
AACGAAT1201.6570993E-917.33252531
CCTCGCC2050.017.1699330
TCCCCGC957.670551E-716.844751
TCCTCGC1900.016.8413229
CGAGCCG2100.016.76112215
AGGCCCG2250.016.35479210
GCCGGTC2350.016.3418838
AGGACCT4250.016.1907775
TAGTACC1304.780304E-916.0025144
AACCGCG805.6121782E-516.0014277
CCTAAGT805.6154393E-516.0003412
Produced by FastQC (version 0.11.5)