##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063014_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	736447
Sequences flagged as poor quality	0
Sequence length	38
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.172947951447966	32.0	32.0	32.0	32.0	32.0
2	31.330966111614277	32.0	32.0	32.0	32.0	32.0
3	31.395895427641094	32.0	32.0	32.0	32.0	32.0
4	31.49695361648564	32.0	32.0	32.0	32.0	32.0
5	31.417511375564025	32.0	32.0	32.0	32.0	32.0
6	34.95171818202803	36.0	36.0	36.0	36.0	36.0
7	34.9667009302774	36.0	36.0	36.0	36.0	36.0
8	34.923846522560346	36.0	36.0	36.0	36.0	36.0
9	35.01233761560574	36.0	36.0	36.0	36.0	36.0
10	34.87879236387683	36.0	36.0	36.0	32.0	36.0
11	35.039857586492985	36.0	36.0	36.0	36.0	36.0
12	34.949957023383895	36.0	36.0	36.0	36.0	36.0
13	34.99297301774602	36.0	36.0	36.0	36.0	36.0
14	34.94182744990474	36.0	36.0	36.0	32.0	36.0
15	34.9229856323673	36.0	36.0	36.0	32.0	36.0
16	34.92546374688199	36.0	36.0	36.0	36.0	36.0
17	34.90240438212118	36.0	36.0	36.0	32.0	36.0
18	34.89470253799662	36.0	36.0	36.0	32.0	36.0
19	34.87964782258601	36.0	36.0	36.0	32.0	36.0
20	34.86925060459205	36.0	36.0	36.0	32.0	36.0
21	34.85533378505174	36.0	36.0	36.0	32.0	36.0
22	34.839745426351115	36.0	36.0	36.0	32.0	36.0
23	34.786383813091774	36.0	36.0	36.0	32.0	36.0
24	34.76630904871634	36.0	36.0	36.0	32.0	36.0
25	34.740170032602485	36.0	36.0	36.0	32.0	36.0
26	34.69092141050204	36.0	36.0	36.0	32.0	36.0
27	34.67595224096235	36.0	36.0	36.0	32.0	36.0
28	34.62823393944167	36.0	36.0	36.0	32.0	36.0
29	34.59510731933187	36.0	36.0	36.0	32.0	36.0
30	34.55857515883696	36.0	36.0	36.0	32.0	36.0
31	34.555037904968046	36.0	36.0	36.0	32.0	36.0
32	34.50967822531696	36.0	36.0	36.0	32.0	36.0
33	34.47502535824031	36.0	36.0	36.0	32.0	36.0
34	34.46153898379652	36.0	36.0	36.0	32.0	36.0
35	34.42588536581723	36.0	36.0	36.0	32.0	36.0
36	34.38945097203193	36.0	36.0	36.0	32.0	36.0
37	34.3619839581124	36.0	36.0	36.0	32.0	36.0
38	33.95420308589756	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	3.0
21	25.0
22	59.0
23	168.0
24	442.0
25	1073.0
26	2440.0
27	4699.0
28	8341.0
29	13342.0
30	20357.0
31	29703.0
32	42178.0
33	66965.0
34	163402.0
35	383246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.71857053424355	18.803835483244544	12.430148821204067	26.04744516130785
2	14.784499088189646	21.21374654252105	38.7778075000645	25.223946869224807
3	17.926476718623334	25.53707191420428	29.48155128610749	27.054900081064897
4	11.589731751861985	16.779121319311013	36.88910916633286	34.74203776249414
5	13.084598191856564	38.08412293637279	33.917082854683166	14.914196017087471
6	32.785883535090875	36.78889801682407	17.215270658365526	13.20994778971953
7	28.627993596280522	31.38134855597212	21.620972045510403	18.36968580223696
8	26.99868012970577	34.75481915604343	19.82317540152844	18.423325312722355
9	27.3608938426107	14.040841046365099	19.022943553643593	39.57532155738061
10	15.561119877029702	27.69642522520565	32.525752337603436	24.216702560161206
11	36.685791106795854	21.150997073621173	23.268174442052945	18.895037377530027
12	24.468086550930483	24.296586982055686	30.01323927788226	21.22208718913157
13	30.047145343989918	20.141327402144896	25.464053033080496	24.347474220784687
14	22.731469788687832	20.858012671669073	26.19866222370683	30.211855315936265
15	24.183953495635123	28.977781157367737	23.178450044606063	23.659815302391074
16	23.994394708648418	26.109278739678484	26.089318036464267	23.80700851520883
17	22.702652057785556	26.726295307062152	26.828407203777054	23.74264543137524
18	23.59748902500791	24.96825976614746	28.89562996386705	22.538621244977573
19	24.941238133905088	25.127130669281023	26.7891647328321	23.14246646398179
20	25.014698953217273	24.909192379084985	26.94111049403419	23.134998173663547
21	25.847993873261238	24.639619577320204	25.660742704129575	23.851643845288983
22	24.811493051076454	25.640339740238577	26.362864843946255	23.18530236473871
23	23.073277705734984	25.33522530535206	27.005546917964	24.58595007094895
24	23.991271605424423	25.7145456495851	26.950479803706173	23.343702941284302
25	23.963875151742286	25.312107065555384	27.018274252287338	23.705743530414995
26	22.955111440621582	26.405737827349192	27.662177539154264	22.97697319287497
27	23.984514818465986	25.781931058081653	26.691977415859412	23.541576707592952
28	22.964313793117494	25.902746565604858	27.093463616526375	24.039476024751274
29	23.327544276777555	25.445551411031612	26.947628274675573	24.27927603751526
30	23.035873593075944	25.673809520576498	27.580667719469286	23.70964916687827
31	23.97769289575489	25.423418114270273	26.412491326599195	24.18639766337564
32	23.206286399428606	26.187899468665087	26.479162791076615	24.126651340829685
33	22.9203187737882	25.586634204498083	27.428721958267193	24.06432506344652
34	23.653161734653004	25.970775901049226	27.33855932606148	23.037503038236288
35	24.099222347297225	25.960320294603683	27.070922958474945	22.869534399624143
36	22.966893747954707	26.319205591169492	27.037926693977976	23.67597396689782
37	23.93709255384298	26.18586266221466	26.302775352469354	23.574269431473006
38	23.45450521218771	26.394160068545325	26.575164268440226	23.576170450826737
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	373.0
1	287.0
2	201.0
3	201.0
4	627.5
5	1054.0
6	1054.0
7	1218.0
8	1382.0
9	1269.0
10	1156.0
11	1156.0
12	1512.0
13	1868.0
14	2332.5
15	2797.0
16	2797.0
17	4290.5
18	5784.0
19	5784.0
20	6538.0
21	7292.0
22	7484.5
23	7677.0
24	7677.0
25	8983.5
26	10290.0
27	10290.0
28	13391.5
29	16493.0
30	20675.0
31	24857.0
32	24857.0
33	30063.5
34	35270.0
35	35270.0
36	38169.5
37	41069.0
38	44590.0
39	48111.0
40	48111.0
41	48889.5
42	49668.0
43	52828.5
44	55989.0
45	55989.0
46	58219.5
47	60450.0
48	60450.0
49	62685.5
50	64921.0
51	63722.0
52	62523.0
53	62523.0
54	58848.5
55	55174.0
56	55174.0
57	52950.0
58	50726.0
59	44981.5
60	39237.0
61	39237.0
62	36782.5
63	34328.0
64	28190.0
65	22052.0
66	22052.0
67	18241.0
68	14430.0
69	14430.0
70	11595.0
71	8760.0
72	6828.0
73	4896.0
74	4896.0
75	3652.5
76	2409.0
77	2409.0
78	2339.0
79	2269.0
80	1739.5
81	1210.0
82	1210.0
83	1180.5
84	1151.0
85	1151.0
86	718.5
87	286.0
88	255.0
89	224.0
90	224.0
91	131.5
92	39.0
93	30.0
94	21.0
95	21.0
96	12.0
97	3.0
98	3.0
99	5.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008147225801720965
2	0.0
3	0.0
4	2.7157419339069887E-4
5	1.3578709669534943E-4
6	2.7157419339069887E-4
7	0.0
8	0.0014936580636488437
9	0.004209399997555832
10	0.0012220838702581447
11	0.0057030580612046754
12	2.7157419339069887E-4
13	1.3578709669534943E-4
14	1.3578709669534943E-4
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	4.073612900860483E-4
22	2.7157419339069887E-4
23	2.7157419339069887E-4
24	0.0
25	1.3578709669534943E-4
26	1.3578709669534943E-4
27	1.3578709669534943E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	736447.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.43713099965334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.50361369187938	50.68785322332787
2	11.863102883039188	14.576700117752878
3	2.8127611682226017	5.184239290884898
4	1.020494591634872	2.507850396428375
5	0.4696773193965135	1.4427813499664812
6	0.2755882255080427	1.0158809947499774
7	0.17123261994040573	0.7364028631884793
8	0.11056038497469532	0.5434010284049969
9	0.08022841088363954	0.443610305041696
>10	0.5271038003580163	6.429249354562167
>50	0.07971023000204411	3.43742554542854
>100	0.0792656916681964	9.363765885658658
>500	0.005772851493436213	2.3172060353498374
>1k	8.881309989901866E-4	1.31363360925525
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3769	0.511781567444772	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2904	0.39432572880329475	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1725	0.23423274179947776	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1232	0.1672897031286705	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	935	0.12696093541015172	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	927	0.12587463863658893	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	808	0.10971597412984234	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	740	0.10048245155455857	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.3578709669534943E-4	0.0
5	0.0	0.0	0.0	1.3578709669534943E-4	0.0
6	0.0	0.0	0.0	1.3578709669534943E-4	0.0
7	0.0	0.0	0.0	1.3578709669534943E-4	0.0
8	0.0	0.0	0.0	1.3578709669534943E-4	0.0
9	0.0	0.0	0.0	1.3578709669534943E-4	0.0
10	0.0	0.0	0.0	1.3578709669534943E-4	0.0
11	0.0	0.0	0.0	1.3578709669534943E-4	0.0
12	0.0	0.0	0.0	1.3578709669534943E-4	0.0
13	0.0	0.0	0.0	1.3578709669534943E-4	0.0
14	0.0	0.0	0.0	1.3578709669534943E-4	0.0
15	0.0	0.0	0.0	1.3578709669534943E-4	1.3578709669534943E-4
16	0.0	0.0	0.0	1.3578709669534943E-4	1.3578709669534943E-4
17	0.0	0.0	0.0	2.7157419339069887E-4	1.3578709669534943E-4
18	0.0	0.0	0.0	2.7157419339069887E-4	1.3578709669534943E-4
19	0.0	0.0	0.0	4.073612900860483E-4	1.3578709669534943E-4
20	0.0	0.0	0.0	4.073612900860483E-4	1.3578709669534943E-4
21	0.0	0.0	0.0	6.789354834767471E-4	4.073612900860483E-4
22	0.0	0.0	0.0	0.0010862967735627955	4.073612900860483E-4
23	0.0	0.0	0.0	0.0020368064504302412	4.073612900860483E-4
24	0.0	0.0	0.0	0.0033946774173837357	5.431483867813977E-4
25	0.0	0.0	0.0	0.003937825804165134	5.431483867813977E-4
26	0.0	0.0	0.0	0.005295696771118628	5.431483867813977E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTAGC	55	5.8286936E-5	20.364069	2
CGGTCCA	210	0.0	19.8086	10
CCGGTCC	240	0.0	18.668331	9
GGTCCAA	255	0.0	18.195229	11
GTCTAGC	45	0.008839341	17.78057	1
GTAATGT	45	0.008839341	17.78057	3
TATTGGG	45	0.00884626	17.778154	2
CGAAGAT	45	0.0088497205	17.776947	30
TCGAACT	55	0.0013644416	17.453732	19
AACGAAT	120	1.6570993E-9	17.332525	31
CCTCGCC	205	0.0	17.16993	30
TCCCCGC	95	7.670551E-7	16.84475	1
TCCTCGC	190	0.0	16.84132	29
CGAGCCG	210	0.0	16.761122	15
AGGCCCG	225	0.0	16.354792	10
GCCGGTC	235	0.0	16.341883	8
AGGACCT	425	0.0	16.190777	5
TAGTACC	130	4.780304E-9	16.002514	4
AACCGCG	80	5.6121782E-5	16.001427	7
CCTAAGT	80	5.6154393E-5	16.000341	2
>>END_MODULE