FastQCFastQC Report
Thu 2 Feb 2017
SRR4063013_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063013_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978505
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT53820.2720235733546289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35010.17695178935610473No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT30240.15284267666748377No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC28970.14642368859315494No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG24870.12570097118784132No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24690.12479119335053487No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24520.12393195872641212No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT24510.12388141551322841No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24300.1228200080363709No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC24280.1227189216100035No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG23130.11690645209387895No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG22020.1112961554304892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT21000.10614074768575263No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG20640.10432119201113974No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20420.1032092413210985No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC20250.10235000669697575No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20210.10214783384424099No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTC400.004491721719.994524
TCTAGCG5750.018.36343428
CTAGCGG6150.017.16993129
TAGCGGC6200.016.77383630
ACGCTTA1601.8189894E-1116.00087417
ATAGCGA600.00244680115.9960196
TACACCG703.7050928E-415.9960195
CAAGACG7400.015.9956154
CGGTCCA7200.015.77863710
AAGACGG7650.015.6823725
CTCGACG1603.0559022E-1015.00119713
CGCAAGA7950.014.894272
ATTTGCC7650.014.85010726
GCCGGTC7550.014.8333718
CGCCGGT7450.014.8177277
CATCGTT4700.014.63687428
GACGCTT1654.838512E-1014.54624816
ATCGTTT4750.014.48353629
GTATCAA45800.014.473551
ACCGTCG4550.014.4165478
Produced by FastQC (version 0.11.5)