FastQCFastQC Report
Thu 2 Feb 2017
SRR4063013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978505
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT58650.29643594532235196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT39820.20126307489746045No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT31350.15845297333087358No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC30970.15653233122989327No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG26370.13328245316539508No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC25990.1313618110644148No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA25800.13040149001392465No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC25720.12999714430845513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT24690.12479119335053487No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT23400.11827111884983862No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23130.11690645209387895No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG22550.11397494572922484No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC22060.11149832828322395No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG21900.11068963687228489No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA21260.10745487122852862No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20650.10437173522432341No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAG308.4131566E-426.6698475
TATACCG502.7963484E-522.402675
TAGACCG400.004481510320.0023845
TACACCG659.4297375E-619.6946565
TCTAGCG6100.019.14692128
CTAGCGG6250.018.17541129
ACGCTTA2150.017.85988617
TAGCGGC6450.017.61183230
CGACGCT2400.017.33277315
AGGGGCG4300.017.11572532
CTCGACG2450.016.97904213
GACGCTT2400.016.66612816
TCGACGC2400.016.66612814
AGCGCGT2350.016.33989732
CGCTTAC2350.016.33989718
TACCGTC5100.016.0015057
AGCGGCG7150.015.88759831
ATACCGT5500.015.7105686
GCGGCGC7850.015.6937632
ACCGTCG5050.015.5262128
Produced by FastQC (version 0.11.5)