##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063013_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1978505
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.210582737976402	32.0	32.0	32.0	32.0	32.0
2	31.303502391957565	32.0	32.0	32.0	32.0	32.0
3	31.39125551868709	32.0	32.0	32.0	32.0	32.0
4	31.483130949883876	32.0	32.0	32.0	32.0	32.0
5	31.412670172680887	32.0	32.0	32.0	32.0	32.0
6	34.94151796432155	36.0	36.0	36.0	36.0	36.0
7	34.96494828165711	36.0	36.0	36.0	36.0	36.0
8	34.90764946259929	36.0	36.0	36.0	36.0	36.0
9	35.019596109183446	36.0	36.0	36.0	36.0	36.0
10	34.86176077391768	36.0	36.0	36.0	32.0	36.0
11	35.0337866217169	36.0	36.0	36.0	36.0	36.0
12	34.93523898094774	36.0	36.0	36.0	32.0	36.0
13	34.984573705904204	36.0	36.0	36.0	36.0	36.0
14	34.93169438540716	36.0	36.0	36.0	32.0	36.0
15	34.90432877349312	36.0	36.0	36.0	32.0	36.0
16	34.91276595206987	36.0	36.0	36.0	32.0	36.0
17	34.879638919285014	36.0	36.0	36.0	32.0	36.0
18	34.87968592447328	36.0	36.0	36.0	32.0	36.0
19	34.87832226858158	36.0	36.0	36.0	32.0	36.0
20	34.8645138627398	36.0	36.0	36.0	32.0	36.0
21	34.84970369041271	36.0	36.0	36.0	32.0	36.0
22	34.8384254778229	36.0	36.0	36.0	32.0	36.0
23	34.78105842542728	36.0	36.0	36.0	32.0	36.0
24	34.758475717776804	36.0	36.0	36.0	32.0	36.0
25	34.7420062117609	36.0	36.0	36.0	32.0	36.0
26	34.6820215263545	36.0	36.0	36.0	32.0	36.0
27	34.667223484398576	36.0	36.0	36.0	32.0	36.0
28	34.63614901150111	36.0	36.0	36.0	32.0	36.0
29	34.602121298657316	36.0	36.0	36.0	32.0	36.0
30	34.57766192150134	36.0	36.0	36.0	32.0	36.0
31	34.57524090158984	36.0	36.0	36.0	32.0	36.0
32	34.548480797369734	36.0	36.0	36.0	32.0	36.0
33	34.51632672143866	36.0	36.0	36.0	32.0	36.0
34	34.511831913490234	36.0	36.0	36.0	32.0	36.0
35	34.47256489116783	36.0	36.0	36.0	32.0	36.0
36	34.442649374148665	36.0	36.0	36.0	32.0	36.0
37	34.439009251935175	36.0	36.0	36.0	32.0	36.0
38	34.013299941117154	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	9.0
21	33.0
22	146.0
23	441.0
24	1237.0
25	2985.0
26	6520.0
27	12506.0
28	22125.0
29	35403.0
30	53530.0
31	77550.0
32	112293.0
33	180983.0
34	446298.0
35	1026442.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98719430147932	18.320782394110612	12.0978821616933	26.594141142716765
2	15.962970001576949	20.281232688940733	36.764848592674056	26.990948716808255
3	17.98822848564952	23.959757493663144	28.75903775830741	29.292976262379927
4	12.000600454586579	15.870348309149199	35.960305301842155	36.16874593442207
5	14.140027010306778	36.93244997230231	33.243400063886654	15.684122953504264
6	34.56984122330935	35.88517979764488	16.252599188679113	13.29237979036665
7	30.573176938679268	30.54032375047953	20.429679266959447	18.45682004388175
8	28.2707257159609	32.95315548456881	19.0715000555988	19.704618743871496
9	27.45737679314167	13.847216065578907	18.403935219700767	40.29147192157866
10	15.819089676282283	26.345791194566747	31.190361504243402	26.644757624907566
11	37.916015470993905	21.107383093106996	21.93031582905886	19.046285606840232
12	24.601351429898553	23.895967494517304	28.283843746035505	23.21883732954864
13	29.26846280398584	19.127725226875846	25.399379834774237	26.20443213436408
14	23.441689558530303	19.777154973073102	24.930894791774598	31.850260676621993
15	25.25341103509973	27.236827806854162	22.084250482055896	25.425510675990203
16	25.92434785289686	25.77529576654673	23.530821029839228	24.769535350717184
17	24.20271871943715	25.979615922123017	25.006002006565563	24.81166335187427
18	25.236327429043648	24.603880202476113	26.13296403092234	24.026828337557905
19	25.496726063366026	24.9144682474899	25.51234391623979	24.07646177290429
20	25.79290929262246	23.977245445424703	25.11264818638315	25.117197075569685
21	27.162772643141125	23.967931293473548	24.42469100359767	24.44460505978766
22	25.797231543242543	24.161550424740323	25.194503116750393	24.846714915266745
23	24.037844812815358	23.976990661111618	25.863772623819752	26.121391902253272
24	24.540701185996497	24.955660966234603	25.43526551613466	25.068372331634237
25	24.762977242378327	24.447435484017706	25.52082332997389	25.26876394363008
26	24.482146350043443	25.384394160635594	25.794906553085845	24.33855293623512
27	25.4747903088443	24.582854225791696	24.89576725861193	25.04658820675207
28	24.293292157462325	24.58962701635831	26.129678722065396	24.987402104113965
29	24.312144775979842	24.82869641471717	25.892580509020704	24.966578300282283
30	24.346514160944754	25.001200401313113	26.08787948476248	24.564405952979648
31	25.10193302518821	24.746715322933223	24.895817801825114	25.255533850053453
32	24.714317123282477	24.821721451297822	24.894301505429606	25.569659919990094
33	24.300671466587147	24.40261712757865	25.548179054387028	25.748532351447178
34	25.04633549068615	24.6559902552685	25.665439308973188	24.632234945072163
35	25.799682083189072	24.385533521522564	25.58249789613875	24.23228649914961
36	24.450279377610872	25.141255645045124	25.26432836914741	25.144136608196593
37	25.72730420191003	24.91411444499761	24.853513132390365	24.505068220701993
38	24.619396199445944	24.62480433419038	25.540345948776373	25.215453517587306
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	61.5
2	90.0
3	90.0
4	440.0
5	790.0
6	790.0
7	1112.0
8	1434.0
9	1536.0
10	1638.0
11	1638.0
12	2106.0
13	2574.0
14	3604.0
15	4634.0
16	4634.0
17	6978.0
18	9322.0
19	9322.0
20	11185.5
21	13049.0
22	14473.0
23	15897.0
24	15897.0
25	18429.5
26	20962.0
27	20962.0
28	26415.0
29	31868.0
30	38856.0
31	45844.0
32	45844.0
33	58379.5
34	70915.0
35	70915.0
36	78132.5
37	85350.0
38	97461.5
39	109573.0
40	109573.0
41	115994.5
42	122416.0
43	138741.0
44	155066.0
45	155066.0
46	157690.5
47	160315.0
48	160315.0
49	169284.5
50	178254.0
51	181380.0
52	184506.0
53	184506.0
54	174927.0
55	165348.0
56	165348.0
57	160282.0
58	155216.0
59	138354.5
60	121493.0
61	121493.0
62	116962.5
63	112432.0
64	94436.0
65	76440.0
66	76440.0
67	64608.5
68	52777.0
69	52777.0
70	42746.0
71	32715.0
72	25748.0
73	18781.0
74	18781.0
75	14261.0
76	9741.0
77	9741.0
78	9077.0
79	8413.0
80	6496.0
81	4579.0
82	4579.0
83	4273.5
84	3968.0
85	3968.0
86	2563.5
87	1159.0
88	911.5
89	664.0
90	664.0
91	399.0
92	134.0
93	99.5
94	65.0
95	65.0
96	43.0
97	21.0
98	21.0
99	25.0
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008440716601676519
2	5.054321318369173E-5
3	0.0
4	2.0217285273476691E-4
5	5.054321318369173E-5
6	1.5162963955107518E-4
7	3.0325927910215036E-4
8	0.0022744445932661277
9	0.0032347656437562706
10	0.001111950690041218
11	0.004700518826083331
12	4.0434570546953383E-4
13	0.0
14	0.0
15	0.0
16	1.0108642636738346E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.5162963955107518E-4
22	1.0108642636738346E-4
23	2.0217285273476691E-4
24	0.0
25	1.5162963955107518E-4
26	1.0108642636738346E-4
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.0217285273476691E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1978505.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.281555261962446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.49519484807071	34.94035032373608
2	14.310915275647018	13.2466283235824
3	4.505266704039343	6.255322497986287
4	1.862996602755794	3.448895208931623
5	0.9477094174715919	2.193073288849686
6	0.5707330076065236	1.584864673882041
7	0.3852220081176494	1.2480071559774797
8	0.2715988519308384	1.005601381977812
9	0.20319132257822387	0.8463609382189775
>10	1.213251819474506	10.398013922871115
>50	0.10918022125811745	3.548769329966215
>100	0.10113252106507406	10.037172905224429
>500	0.015372260455177375	4.90591974159673
>1k	0.008125337669165185	6.04297268619142
>5k	1.0980186039412411E-4	0.2980476210077256
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5865	0.29643594532235196	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3982	0.20126307489746045	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3135	0.15845297333087358	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3097	0.15653233122989327	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2637	0.13328245316539508	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2599	0.1313618110644148	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2580	0.13040149001392465	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2572	0.12999714430845513	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2469	0.12479119335053487	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2340	0.11827111884983862	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2313	0.11690645209387895	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2255	0.11397494572922484	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2206	0.11149832828322395	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2190	0.11068963687228489	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2126	0.10745487122852862	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2065	0.10437173522432341	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5162963955107518E-4
2	0.0	0.0	0.0	5.054321318369173E-5	1.5162963955107518E-4
3	0.0	0.0	0.0	5.054321318369173E-5	1.5162963955107518E-4
4	0.0	0.0	0.0	5.054321318369173E-5	1.5162963955107518E-4
5	0.0	5.054321318369173E-5	0.0	5.054321318369173E-5	1.5162963955107518E-4
6	0.0	5.054321318369173E-5	0.0	5.054321318369173E-5	1.5162963955107518E-4
7	0.0	5.054321318369173E-5	0.0	5.054321318369173E-5	1.5162963955107518E-4
8	0.0	5.054321318369173E-5	0.0	5.054321318369173E-5	1.5162963955107518E-4
9	0.0	5.054321318369173E-5	0.0	5.054321318369173E-5	1.5162963955107518E-4
10	0.0	5.054321318369173E-5	0.0	5.054321318369173E-5	2.0217285273476691E-4
11	0.0	5.054321318369173E-5	0.0	1.0108642636738346E-4	2.0217285273476691E-4
12	0.0	5.054321318369173E-5	0.0	1.0108642636738346E-4	2.0217285273476691E-4
13	0.0	5.054321318369173E-5	0.0	1.0108642636738346E-4	2.527160659184586E-4
14	0.0	5.054321318369173E-5	0.0	1.5162963955107518E-4	2.527160659184586E-4
15	0.0	5.054321318369173E-5	0.0	2.527160659184586E-4	3.538024922858421E-4
16	0.0	5.054321318369173E-5	0.0	4.0434570546953383E-4	3.538024922858421E-4
17	0.0	5.054321318369173E-5	0.0	4.5488891865322556E-4	3.538024922858421E-4
18	0.0	5.054321318369173E-5	0.0	5.55975345020609E-4	3.538024922858421E-4
19	0.0	5.054321318369173E-5	0.0	5.55975345020609E-4	3.538024922858421E-4
20	0.0	5.054321318369173E-5	0.0	6.065185582043007E-4	4.0434570546953383E-4
21	0.0	5.054321318369173E-5	0.0	6.065185582043007E-4	4.0434570546953383E-4
22	0.0	1.0108642636738346E-4	0.0	7.581481977553759E-4	4.0434570546953383E-4
23	0.0	1.0108642636738346E-4	0.0	9.603210504901428E-4	4.0434570546953383E-4
24	0.0	1.0108642636738346E-4	0.0	0.0012635803295922932	4.0434570546953383E-4
25	0.0	1.0108642636738346E-4	0.0	0.0015668396086944436	4.0434570546953383E-4
26	0.0	1.0108642636738346E-4	0.0	0.0017184692482455188	4.5488891865322556E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGAG	30	8.4131566E-4	26.669847	5
TATACCG	50	2.7963484E-5	22.40267	5
TAGACCG	40	0.0044815103	20.002384	5
TACACCG	65	9.4297375E-6	19.694656	5
TCTAGCG	610	0.0	19.146921	28
CTAGCGG	625	0.0	18.175411	29
ACGCTTA	215	0.0	17.859886	17
TAGCGGC	645	0.0	17.611832	30
CGACGCT	240	0.0	17.332773	15
AGGGGCG	430	0.0	17.115725	32
CTCGACG	245	0.0	16.979042	13
GACGCTT	240	0.0	16.666128	16
TCGACGC	240	0.0	16.666128	14
AGCGCGT	235	0.0	16.339897	32
CGCTTAC	235	0.0	16.339897	18
TACCGTC	510	0.0	16.001505	7
AGCGGCG	715	0.0	15.887598	31
ATACCGT	550	0.0	15.710568	6
GCGGCGC	785	0.0	15.69376	32
ACCGTCG	505	0.0	15.526212	8
>>END_MODULE