FastQCFastQC Report
Thu 2 Feb 2017
SRR4063012_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063012_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677775
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27580.4069197004905758No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19520.2880011803327063No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15530.22913208660691234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11830.1745416989413891No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11490.16952528493969238No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9090.13411530375124486No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.12998413927925936No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC7350.10844306738962044No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT7120.1050496108590609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG751.4910001E-619.2210081
AACGTAT751.5068999E-619.19973621
TATAAGG601.1329292E-418.6760542
TATACAA701.7868455E-518.2841135
GACGGAC1401.8189894E-1218.2827647
CGAACGA802.7931164E-617.99975216
TCTAGCG1006.808659E-817.59845728
CTAGCGG1006.808659E-817.59845729
CGCTTCG1101.061926E-817.45301632
CGCAATC652.0990234E-417.2305312
ATAATAC652.1002073E-417.229263
CATATTA854.9785012E-616.9409439
CCGATAA957.682047E-716.8418719
ATTTAGA703.6629688E-416.0175081
GTCCTAG805.5631106E-516.0175081
TTCTATA1304.7566573E-916.0080472
CAACGTA805.6160636E-515.99977920
TCTTTCG1203.1037416E-815.99977910
CGATAAC1001.3126119E-615.99977910
ACGTTTT1001.3136232E-615.99859829
Produced by FastQC (version 0.11.5)