##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063012_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	677775
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.185501088119214	32.0	32.0	32.0	32.0	32.0
2	30.81572203164767	32.0	32.0	32.0	32.0	32.0
3	30.884653461694516	32.0	32.0	32.0	32.0	32.0
4	30.933888089705285	32.0	32.0	32.0	32.0	32.0
5	30.86436501788942	32.0	32.0	32.0	32.0	32.0
6	34.50487256095312	36.0	36.0	36.0	32.0	36.0
7	34.42329386595847	36.0	36.0	36.0	32.0	36.0
8	34.38496993840139	36.0	36.0	36.0	32.0	36.0
9	34.52703920917709	36.0	36.0	36.0	32.0	36.0
10	34.26611633654236	36.0	36.0	36.0	32.0	36.0
11	34.54532403821327	36.0	36.0	36.0	32.0	36.0
12	34.35345948139132	36.0	36.0	36.0	32.0	36.0
13	34.4480011803327	36.0	36.0	36.0	32.0	36.0
14	34.35219800081148	36.0	36.0	36.0	32.0	36.0
15	34.30624322230829	36.0	36.0	36.0	32.0	36.0
16	34.309418317288184	36.0	36.0	36.0	32.0	36.0
17	34.23844491165947	36.0	36.0	36.0	32.0	36.0
18	34.25440005901663	36.0	36.0	36.0	32.0	36.0
19	34.24771642506732	36.0	36.0	36.0	32.0	36.0
20	34.19975213013168	36.0	36.0	36.0	32.0	36.0
21	34.19055733834975	36.0	36.0	36.0	32.0	36.0
22	34.162815093504484	36.0	36.0	36.0	32.0	36.0
23	34.112894397108185	36.0	36.0	36.0	32.0	36.0
24	34.089739220242706	36.0	36.0	36.0	32.0	36.0
25	34.07816458264173	36.0	36.0	36.0	32.0	36.0
26	34.02062631404227	36.0	36.0	36.0	32.0	36.0
27	34.036212607428716	36.0	36.0	36.0	32.0	36.0
28	33.99920032459149	36.0	36.0	36.0	32.0	36.0
29	33.97382317140644	36.0	36.0	36.0	32.0	36.0
30	33.926952159639995	36.0	36.0	36.0	32.0	36.0
31	33.933747925196414	36.0	36.0	36.0	32.0	36.0
32	33.902324517723436	36.0	36.0	36.0	32.0	36.0
33	33.84852937921877	36.0	36.0	36.0	32.0	36.0
34	33.838201468038804	36.0	36.0	36.0	32.0	36.0
35	33.78777175316292	36.0	36.0	36.0	32.0	36.0
36	33.73064365017889	36.0	36.0	36.0	27.0	36.0
37	33.72166721994762	36.0	36.0	36.0	27.0	36.0
38	33.10707830769798	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	2.0
9	0.0
10	9.0
11	9.0
12	4.0
13	6.0
14	78.0
15	189.0
16	268.0
17	321.0
18	389.0
19	541.0
20	702.0
21	1013.0
22	1496.0
23	2171.0
24	3108.0
25	4746.0
26	6766.0
27	9772.0
28	13778.0
29	18433.0
30	24695.0
31	33236.0
32	44741.0
33	66325.0
34	145476.0
35	299498.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.11272370063445	17.092907314262924	11.382272347905726	25.412096637196903
2	16.152032677548355	19.824123459013563	38.25041666236103	25.773427201077048
3	19.171710792313846	24.506334792219405	28.411240604055564	27.910713811411185
4	12.107856879380302	15.370416820361491	36.33198081888602	36.189745481372185
5	13.916016129294025	37.32547622876686	33.97051815283288	14.787989489106234
6	33.81781430278708	35.788394293049265	17.15404930876256	13.239742095401095
7	29.85607318062779	30.723175094979897	20.968315443915753	18.452436280476558
8	28.421187749808563	33.23698782328493	19.518301958336835	18.823522468569674
9	27.58165344444231	14.006606931819354	18.6556560972479	39.756083526490436
10	15.570473759534945	27.160246101184764	32.145418062174485	25.1238620771058
11	37.416747570666814	21.441281401326137	21.974657621156815	19.167313406850234
12	24.419882818321184	24.109444323947884	29.434261255239758	22.036411602491178
13	29.986202825838344	19.68539491644225	25.330873943999705	24.9975283137197
14	23.2906749061913	20.028065143003857	25.771238097767622	30.910021853037218
15	25.001033015164666	27.39718547639708	23.352340812363124	24.249440696075133
16	25.130256558570814	26.01147113550076	24.835583834168755	24.022688471759672
17	23.41393517616712	26.281926416508767	25.968491201973876	24.335647205350238
18	24.41842964939047	25.03386284294428	27.285631235853597	23.262076271811654
19	24.843409937659082	25.56907300158619	26.227599690139808	23.359917370614923
20	25.1894937046923	24.516213755686287	26.40384697673252	23.890445562888893
21	26.31255108996851	24.612601552839937	25.026928286652716	24.047919070538832
22	24.950901769602034	25.0881259747714	25.768639990084445	24.192332265542117
23	23.759607737244156	24.9719274791983	26.07269491773018	25.195769865827366
24	24.038077024668024	25.795988956423322	26.106165308353958	24.059768710554703
25	24.513219619665705	24.94956962637992	26.005840626738504	24.531370127215872
26	23.68382218391958	25.986801329311554	26.591248502163378	23.738127984605487
27	24.6754166574445	25.538463808533084	25.867510679267834	23.91860885475458
28	23.745591902380003	25.37869062901155	26.41199297655408	24.463724492054357
29	23.544592937551737	25.75738124855208	26.703969473083106	23.994056340813078
30	23.941305358209945	25.79436218636803	26.57961327637976	23.684719179042265
31	24.04623066344809	25.75575596253386	25.70027680558097	24.49773656843708
32	23.65369434505146	25.977055590394333	25.626913571138736	24.742336493415472
33	23.457170850326687	25.486339410608572	26.223078693730116	24.833411045334625
34	24.073655987758432	25.766462886477758	26.512820739835707	23.6470603859281
35	24.708559353547756	25.746973444223425	26.272156867374584	23.272310334854236
36	23.72151293538505	26.49525732060749	25.740471847903716	24.04275789610374
37	24.473441128991798	26.246639378369846	25.65478632201174	23.625133170626615
38	23.670635798916972	26.254703200389535	25.886857598158556	24.187803402534932
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	117.0
1	139.5
2	162.0
3	162.0
4	431.0
5	700.0
6	700.0
7	842.5
8	985.0
9	979.0
10	973.0
11	973.0
12	1291.0
13	1609.0
14	1962.0
15	2315.0
16	2315.0
17	3413.5
18	4512.0
19	4512.0
20	5275.0
21	6038.0
22	6309.0
23	6580.0
24	6580.0
25	7651.5
26	8723.0
27	8723.0
28	10945.5
29	13168.0
30	16332.5
31	19497.0
32	19497.0
33	22872.5
34	26248.0
35	26248.0
36	28921.0
37	31594.0
38	35679.0
39	39764.0
40	39764.0
41	42056.0
42	44348.0
43	48727.5
44	53107.0
45	53107.0
46	56507.5
47	59908.0
48	59908.0
49	60644.0
50	61380.0
51	60309.0
52	59238.0
53	59238.0
54	55867.0
55	52496.0
56	52496.0
57	50667.0
58	48838.0
59	44212.5
60	39587.0
61	39587.0
62	36811.0
63	34035.0
64	28392.5
65	22750.0
66	22750.0
67	18912.5
68	15075.0
69	15075.0
70	12228.5
71	9382.0
72	7346.0
73	5310.0
74	5310.0
75	4071.0
76	2832.0
77	2832.0
78	2628.5
79	2425.0
80	1904.0
81	1383.0
82	1383.0
83	1315.0
84	1247.0
85	1247.0
86	832.0
87	417.0
88	357.5
89	298.0
90	298.0
91	219.0
92	140.0
93	136.0
94	132.0
95	132.0
96	118.0
97	104.0
98	104.0
99	231.0
100	358.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11950868651101028
2	0.054295304488952825
3	0.013131201357382612
4	0.0036885397071299477
5	2.950831765703958E-4
6	7.377079414259895E-4
7	0.0
8	8.852495297111873E-4
9	2.950831765703958E-4
10	7.377079414259895E-4
11	0.002508207000848364
12	0.003540998118844749
13	0.01475415882851979
14	0.009737744826823061
15	0.021688613477924088
16	0.010918077533104644
17	0.01814761535907934
18	0.006049205119693114
19	0.007377079414259895
20	0.007081996237689498
21	0.007229537825974697
22	0.00767216259083029
23	0.009737744826823061
24	0.014163992475378997
25	0.015934491534801372
26	0.019327948065360923
27	0.008852495297111873
28	0.0066393714728339055
29	0.011508243886245436
30	0.004426247648555937
31	0.0063442882962635085
32	0.007377079414259895
33	0.008704953708826676
34	0.012393493415956624
35	0.015786949946516173
36	0.014606617240234591
37	0.010770535944819446
38	0.0066393714728339055
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	677775.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.58394450612019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.65795336651425	48.2599302622801
2	13.063943742922707	15.829304855046109
3	3.7799502488529257	6.8701288833720255
4	1.3775715435562916	3.338348717920988
5	0.6603542922495659	2.0003433898012983
6	0.3449900718973454	1.2540515622594712
7	0.21418085068531179	0.9083144540534781
8	0.12945724800034888	0.6274424583014541
9	0.09691588043919028	0.5284391690060715
>10	0.5209916232510357	6.1694259547044235
>50	0.07978242863837186	3.47080181935149
>100	0.06876935609584343	7.976054189276913
>500	0.003915692873718542	1.4930544787670315
>1k	0.0012236540230370443	1.274359805859249
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2758	0.4069197004905758	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1952	0.2880011803327063	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1553	0.22913208660691234	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1183	0.1745416989413891	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1149	0.16952528493969238	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	909	0.13411530375124486	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	881	0.12998413927925936	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	735	0.10844306738962044	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	712	0.1050496108590609	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.950831765703958E-4
2	0.0	0.0	0.0	0.0	2.950831765703958E-4
3	0.0	0.0	0.0	0.0	2.950831765703958E-4
4	0.0	0.0	0.0	0.0	2.950831765703958E-4
5	0.0	0.0	0.0	0.0	2.950831765703958E-4
6	0.0	0.0	0.0	0.0	2.950831765703958E-4
7	0.0	0.0	0.0	0.0	2.950831765703958E-4
8	0.0	0.0	0.0	0.0	2.950831765703958E-4
9	0.0	0.0	0.0	0.0	2.950831765703958E-4
10	0.0	0.0	0.0	0.0	2.950831765703958E-4
11	0.0	0.0	0.0	0.0	2.950831765703958E-4
12	0.0	0.0	0.0	0.0	2.950831765703958E-4
13	0.0	0.0	0.0	0.0	2.950831765703958E-4
14	0.0	0.0	0.0	0.0	2.950831765703958E-4
15	0.0	0.0	0.0	0.0	4.4262476485559365E-4
16	0.0	0.0	0.0	0.0	4.4262476485559365E-4
17	0.0	0.0	0.0	0.0	4.4262476485559365E-4
18	0.0	0.0	0.0	1.475415882851979E-4	4.4262476485559365E-4
19	0.0	0.0	0.0	1.475415882851979E-4	4.4262476485559365E-4
20	0.0	0.0	0.0	1.475415882851979E-4	5.901663531407916E-4
21	0.0	0.0	0.0	5.901663531407916E-4	5.901663531407916E-4
22	0.0	0.0	0.0	0.0011803327062815832	5.901663531407916E-4
23	0.0	0.0	0.0	0.002508207000848364	7.377079414259895E-4
24	0.0	0.0	0.0	0.0036885397071299473	7.377079414259895E-4
25	0.0	0.0	0.0	0.004721330825126333	7.377079414259895E-4
26	0.0	0.0	0.0	0.005459038766552322	7.377079414259895E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGG	75	1.4910001E-6	19.221008	1
AACGTAT	75	1.5068999E-6	19.199736	21
TATAAGG	60	1.1329292E-4	18.676054	2
TATACAA	70	1.7868455E-5	18.284113	5
GACGGAC	140	1.8189894E-12	18.282764	7
CGAACGA	80	2.7931164E-6	17.999752	16
TCTAGCG	100	6.808659E-8	17.598457	28
CTAGCGG	100	6.808659E-8	17.598457	29
CGCTTCG	110	1.061926E-8	17.453016	32
CGCAATC	65	2.0990234E-4	17.23053	12
ATAATAC	65	2.1002073E-4	17.22926	3
CATATTA	85	4.9785012E-6	16.940943	9
CCGATAA	95	7.682047E-7	16.841871	9
ATTTAGA	70	3.6629688E-4	16.017508	1
GTCCTAG	80	5.5631106E-5	16.017508	1
TTCTATA	130	4.7566573E-9	16.008047	2
CAACGTA	80	5.6160636E-5	15.999779	20
TCTTTCG	120	3.1037416E-8	15.999779	10
CGATAAC	100	1.3126119E-6	15.999779	10
ACGTTTT	100	1.3136232E-6	15.998598	29
>>END_MODULE