FastQCFastQC Report
Thu 2 Feb 2017
SRR4063012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677775
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32240.47567408063147804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24330.3589686842978865No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15710.23178783519604587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15580.2298697945483383No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14660.2162959684261001No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10610.15654162517059494No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC8150.12024639445243628No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT7420.10947585850761685No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7290.10755781785990925No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG6820.10062336321050495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG1300.023.3896311
CTAGCGG1350.021.33318529
TCTAGCG1300.020.92293428
CTAAGAC951.720764E-920.2103863
GTACCGT400.004481794319.9998636
TACACGG400.004481794319.9998635
TAGCGGC1450.019.8619330
TATTAGC953.7829523E-818.5261882
TTAGAGT1055.9644663E-918.2855874
GTATAAT550.001361904117.4582881
TCCCCGC652.0953648E-417.2344651
GTAAACG1404.0017767E-1117.14273827
AGCGGCG1900.016.8419931
TAATAGT1051.1870361E-716.761794
CGCTTCG1456.91216E-1116.55160932
AACGTAT600.002440630715.99988931
CCTAATA600.002440630715.9998892
ATCACGA1501.1459633E-1015.99988925
CCGTCGT1304.789399E-915.9998899
AAACGCT1501.1459633E-1015.99988929
Produced by FastQC (version 0.11.5)