##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063012_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	677775
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13056987938475	32.0	32.0	32.0	32.0	32.0
2	31.27993803253292	32.0	32.0	32.0	32.0	32.0
3	31.37652318247206	32.0	32.0	32.0	32.0	32.0
4	31.479712293902843	32.0	32.0	32.0	32.0	32.0
5	31.399222455829737	32.0	32.0	32.0	32.0	32.0
6	34.920789347497326	36.0	36.0	36.0	36.0	36.0
7	34.95744015344325	36.0	36.0	36.0	36.0	36.0
8	34.89964073623253	36.0	36.0	36.0	36.0	36.0
9	35.016939249751026	36.0	36.0	36.0	36.0	36.0
10	34.86110729962008	36.0	36.0	36.0	32.0	36.0
11	35.033912434067354	36.0	36.0	36.0	36.0	36.0
12	34.93926007893475	36.0	36.0	36.0	36.0	36.0
13	34.97973073660138	36.0	36.0	36.0	36.0	36.0
14	34.91941573531039	36.0	36.0	36.0	32.0	36.0
15	34.90800781970418	36.0	36.0	36.0	32.0	36.0
16	34.907219947622735	36.0	36.0	36.0	32.0	36.0
17	34.87574785142562	36.0	36.0	36.0	32.0	36.0
18	34.87560916233263	36.0	36.0	36.0	32.0	36.0
19	34.86804470510125	36.0	36.0	36.0	32.0	36.0
20	34.84737560399838	36.0	36.0	36.0	32.0	36.0
21	34.839154586699124	36.0	36.0	36.0	32.0	36.0
22	34.81213677105234	36.0	36.0	36.0	32.0	36.0
23	34.756459001881154	36.0	36.0	36.0	32.0	36.0
24	34.72691379882704	36.0	36.0	36.0	32.0	36.0
25	34.70732322673454	36.0	36.0	36.0	32.0	36.0
26	34.64819593522924	36.0	36.0	36.0	32.0	36.0
27	34.62490797093431	36.0	36.0	36.0	32.0	36.0
28	34.59183209767253	36.0	36.0	36.0	32.0	36.0
29	34.5504835675556	36.0	36.0	36.0	32.0	36.0
30	34.520581313857846	36.0	36.0	36.0	32.0	36.0
31	34.50823945999779	36.0	36.0	36.0	32.0	36.0
32	34.478201468038804	36.0	36.0	36.0	32.0	36.0
33	34.430657666629784	36.0	36.0	36.0	32.0	36.0
34	34.41162480174099	36.0	36.0	36.0	32.0	36.0
35	34.359045405923794	36.0	36.0	36.0	32.0	36.0
36	34.32470215041865	36.0	36.0	36.0	32.0	36.0
37	34.305906827487	36.0	36.0	36.0	32.0	36.0
38	33.86979159750655	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	5.0
21	10.0
22	50.0
23	157.0
24	433.0
25	1081.0
26	2316.0
27	4586.0
28	7915.0
29	12555.0
30	19381.0
31	27719.0
32	40168.0
33	62959.0
34	153245.0
35	345194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.681156127021694	18.26043844647645	12.113570533752588	25.94483489274927
2	15.313193906532405	20.79333112020951	37.45830105861089	26.43517391464719
3	18.233484563461325	25.079856884659364	28.77488842167386	27.911770130205454
4	11.698737337224504	16.212483807286795	36.34471077379776	35.74406808169095
5	13.48074726973888	37.68055428505667	33.78943423619082	15.049264209013625
6	33.82818782044188	36.22544354689978	16.721183283538046	13.225185349120283
7	29.531672799487733	30.784007648567226	21.085494574887793	18.598824977057248
8	28.14324961564225	33.4842730548252	19.36528592970311	19.007191399829438
9	27.387035138066118	13.903844309521876	18.39986425773325	40.30925629467876
10	15.325280442156547	27.420551839933665	32.30968073193079	24.944486985978997
11	37.586188071113554	21.31016196643811	21.92471076367832	19.178939198770017
12	24.154547318940118	24.29426961534319	29.30376394456551	22.247419121151182
13	29.985850719931307	19.688597804869772	25.26096495434312	25.0645865208558
14	23.072553576039244	19.929770203976247	25.808859872376527	31.188816347607982
15	24.94234812437756	27.523735753015384	23.223045996090146	24.31087012651691
16	25.03574194976209	26.153664564199037	24.655674818339417	24.15491866769946
17	23.291652834642765	26.1968942495666	26.092582346648964	24.418870569141678
18	24.378886798716387	24.947954704732396	27.459850245287893	23.213308251263324
19	24.853675629818156	25.650547748146508	26.229943565342477	23.265833056692856
20	25.104496329902993	24.45767400686068	26.605879532293166	23.83195013094316
21	26.210064121668875	24.652170192424023	25.14023848657517	23.99752719933193
22	24.92080705248792	24.993545055512524	25.9376636789495	24.14798421305005
23	23.69975286783962	24.713216037770646	26.380140902216816	25.20689019217292
24	24.031278816716462	25.684187230275533	26.18568108885692	24.098852864151084
25	24.550550110435204	24.92442159838176	26.11582343941113	24.409204851771907
26	23.757736712035705	25.54815388587658	26.834126369370363	23.859983032717345
27	24.60672731618504	25.172550142082763	26.10014547622069	24.12057706551151
28	23.68293312677511	25.11954557190808	26.792667183062225	24.404854118254583
29	23.525063627309947	25.4004647560031	26.921175906458632	24.15329571022832
30	23.989377005643465	25.324185754859652	26.85301169267087	23.83342554682601
31	24.092361034266535	25.352661281398692	25.91140127623474	24.64357640810003
32	23.73767105602892	25.605400022131235	25.95772933495629	24.69919958688355
33	23.544391575375307	25.165726089041346	26.394747519457045	24.895134816126298
34	24.082623289439713	25.613662351075213	26.63066651912508	23.673047840360002
35	24.79879015897606	25.447530522666078	26.409649219873856	23.344030098484012
36	23.80613035299325	26.113680793773746	26.057762531813655	24.02242632141935
37	24.64018295156947	25.79189258972373	25.884106082401974	23.68381837630482
38	23.743642783055684	25.786214558561642	26.164364765194247	24.305777893188427
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	37.0
1	62.5
2	88.0
3	88.0
4	345.5
5	603.0
6	603.0
7	789.5
8	976.0
9	1045.0
10	1114.0
11	1114.0
12	1311.0
13	1508.0
14	1954.0
15	2400.0
16	2400.0
17	3629.5
18	4859.0
19	4859.0
20	5522.5
21	6186.0
22	6527.5
23	6869.0
24	6869.0
25	7758.5
26	8648.0
27	8648.0
28	11047.5
29	13447.0
30	16179.5
31	18912.0
32	18912.0
33	22688.5
34	26465.0
35	26465.0
36	29005.0
37	31545.0
38	35625.5
39	39706.0
40	39706.0
41	41616.5
42	43527.0
43	47975.0
44	52423.0
45	52423.0
46	56388.5
47	60354.0
48	60354.0
49	61245.0
50	62136.0
51	61004.0
52	59872.0
53	59872.0
54	56474.5
55	53077.0
56	53077.0
57	50832.0
58	48587.0
59	44109.0
60	39631.0
61	39631.0
62	37047.0
63	34463.0
64	28573.5
65	22684.0
66	22684.0
67	18987.5
68	15291.0
69	15291.0
70	12240.0
71	9189.0
72	7239.0
73	5289.0
74	5289.0
75	3981.5
76	2674.0
77	2674.0
78	2524.0
79	2374.0
80	1817.0
81	1260.0
82	1260.0
83	1153.5
84	1047.0
85	1047.0
86	667.5
87	288.0
88	230.0
89	172.0
90	172.0
91	105.5
92	39.0
93	27.5
94	16.0
95	16.0
96	12.5
97	9.0
98	9.0
99	9.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009442661650252666
2	0.0
3	0.0
4	1.475415882851979E-4
5	1.475415882851979E-4
6	0.0
7	1.475415882851979E-4
8	0.003098373353989156
9	0.002950831765703958
10	8.852495297111873E-4
11	0.006196746707978312
12	2.950831765703958E-4
13	2.950831765703958E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.475415882851979E-4
22	0.0
23	0.0
24	0.0
25	2.950831765703958E-4
26	0.0
27	1.475415882851979E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.950831765703958E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	677775.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.46965033484999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.09768811612457	45.66344516109973
2	14.088152139102023	16.474586588749286
3	4.006511746557492	7.02778022651057
4	1.5272268700270042	3.5718568428986535
5	0.7062658633552418	2.0647559036910965
6	0.3740792995441352	1.3123371505110717
7	0.21174362604204588	0.8666403042718162
8	0.15439007380738812	0.7221706904551732
9	0.11057222132454757	0.5818607205834545
>10	0.5578019043668893	6.416527311075279
>50	0.08354595835052939	3.4523926830625697
>100	0.07618159262991195	8.619388899686403
>500	0.004316956915656708	1.546536808011842
>1k	0.0015236318525847204	1.6797207093930462
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3224	0.47567408063147804	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2433	0.3589686842978865	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1571	0.23178783519604587	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1558	0.2298697945483383	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1466	0.2162959684261001	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1061	0.15654162517059494	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	815	0.12024639445243628	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	742	0.10947585850761685	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	729	0.10755781785990925	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	682	0.10062336321050495	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.475415882851979E-4
2	0.0	0.0	0.0	0.0	1.475415882851979E-4
3	0.0	0.0	0.0	0.0	1.475415882851979E-4
4	0.0	0.0	0.0	0.0	1.475415882851979E-4
5	0.0	0.0	0.0	0.0	1.475415882851979E-4
6	0.0	0.0	0.0	0.0	1.475415882851979E-4
7	0.0	0.0	0.0	0.0	1.475415882851979E-4
8	0.0	0.0	0.0	0.0	1.475415882851979E-4
9	0.0	0.0	0.0	1.475415882851979E-4	2.950831765703958E-4
10	0.0	0.0	0.0	1.475415882851979E-4	2.950831765703958E-4
11	0.0	0.0	0.0	1.475415882851979E-4	2.950831765703958E-4
12	0.0	0.0	0.0	1.475415882851979E-4	2.950831765703958E-4
13	0.0	0.0	0.0	1.475415882851979E-4	2.950831765703958E-4
14	0.0	0.0	0.0	1.475415882851979E-4	2.950831765703958E-4
15	0.0	0.0	0.0	1.475415882851979E-4	2.950831765703958E-4
16	0.0	0.0	0.0	1.475415882851979E-4	4.4262476485559365E-4
17	0.0	0.0	0.0	1.475415882851979E-4	4.4262476485559365E-4
18	0.0	0.0	0.0	2.950831765703958E-4	5.901663531407916E-4
19	0.0	0.0	0.0	2.950831765703958E-4	5.901663531407916E-4
20	0.0	0.0	0.0	2.950831765703958E-4	5.901663531407916E-4
21	0.0	0.0	0.0	7.377079414259895E-4	5.901663531407916E-4
22	0.0	0.0	0.0	0.001475415882851979	5.901663531407916E-4
23	0.0	0.0	0.0	0.003098373353989156	7.377079414259895E-4
24	0.0	0.0	0.0	0.004426247648555937	7.377079414259895E-4
25	0.0	0.0	0.0	0.005459038766552322	7.377079414259895E-4
26	0.0	0.0	0.0	0.006196746707978312	7.377079414259895E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	130	0.0	23.389631	1
CTAGCGG	135	0.0	21.333185	29
TCTAGCG	130	0.0	20.922934	28
CTAAGAC	95	1.720764E-9	20.210386	3
GTACCGT	40	0.0044817943	19.999863	6
TACACGG	40	0.0044817943	19.999863	5
TAGCGGC	145	0.0	19.86193	30
TATTAGC	95	3.7829523E-8	18.526188	2
TTAGAGT	105	5.9644663E-9	18.285587	4
GTATAAT	55	0.0013619041	17.458288	1
TCCCCGC	65	2.0953648E-4	17.234465	1
GTAAACG	140	4.0017767E-11	17.142738	27
AGCGGCG	190	0.0	16.84199	31
TAATAGT	105	1.1870361E-7	16.76179	4
CGCTTCG	145	6.91216E-11	16.551609	32
AACGTAT	60	0.0024406307	15.999889	31
CCTAATA	60	0.0024406307	15.999889	2
ATCACGA	150	1.1459633E-10	15.999889	25
CCGTCGT	130	4.789399E-9	15.999889	9
AAACGCT	150	1.1459633E-10	15.999889	29
>>END_MODULE