FastQCFastQC Report
Thu 2 Feb 2017
SRR4063011_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063011_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844472
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42120.22835803416912806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28210.15294349819352096No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22290.12084759215645452No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20690.11217302295724739No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20100.10897427556503976No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19290.10458277490794114No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18590.10078765088328802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA400.00448374220.0005769
CTAGCGG4450.017.97658529
TCTAGCG4550.017.58149528
GTATCAA31300.016.8316121
TAGCGGC5000.015.99915930
CATCGTT5000.015.99915928
TACGCTG703.7055044E-415.995695
CGGCATC5150.015.53400725
TAGACCC1357.941708E-915.4040924
CGCGGTC2600.015.38505910
GTATTAG3600.015.123441
ATCGTTT5300.015.09354729
AGCGGCG5200.015.07613131
CGTTATT3200.015.0040942
TCGTTTA5350.014.95248530
CTACGCT650.00416880614.7660524
GCATCGT5650.014.72489127
CGGTCCA5600.014.57184910
AATAGCG3950.014.1733965
GTTATTC3500.014.1687633
Produced by FastQC (version 0.11.5)