##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063011_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1844472
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23917522196054	32.0	32.0	32.0	32.0	32.0
2	30.802417168707358	32.0	32.0	32.0	32.0	32.0
3	30.845970554174855	32.0	32.0	32.0	32.0	32.0
4	30.929226358545968	32.0	32.0	32.0	32.0	32.0
5	30.823667694603117	32.0	32.0	32.0	32.0	32.0
6	34.48783229021639	36.0	36.0	36.0	32.0	36.0
7	34.419107473575096	36.0	36.0	36.0	32.0	36.0
8	34.404593292823094	36.0	36.0	36.0	32.0	36.0
9	34.56619780620145	36.0	36.0	36.0	32.0	36.0
10	34.25168883019097	36.0	36.0	36.0	32.0	36.0
11	34.54806904089626	36.0	36.0	36.0	32.0	36.0
12	34.34314264461591	36.0	36.0	36.0	32.0	36.0
13	34.44920443357232	36.0	36.0	36.0	32.0	36.0
14	34.342242115900916	36.0	36.0	36.0	32.0	36.0
15	34.272843935825534	36.0	36.0	36.0	32.0	36.0
16	34.291545764858455	36.0	36.0	36.0	32.0	36.0
17	34.22433574486357	36.0	36.0	36.0	32.0	36.0
18	34.24309666939916	36.0	36.0	36.0	32.0	36.0
19	34.23238628724101	36.0	36.0	36.0	32.0	36.0
20	34.214814320846294	36.0	36.0	36.0	32.0	36.0
21	34.21585038970502	36.0	36.0	36.0	32.0	36.0
22	34.18769219592382	36.0	36.0	36.0	32.0	36.0
23	34.12425073408542	36.0	36.0	36.0	32.0	36.0
24	34.110073235050464	36.0	36.0	36.0	32.0	36.0
25	34.091166469320214	36.0	36.0	36.0	32.0	36.0
26	34.04311423540178	36.0	36.0	36.0	32.0	36.0
27	34.051658686063	36.0	36.0	36.0	32.0	36.0
28	34.03313956514385	36.0	36.0	36.0	32.0	36.0
29	33.99594789186282	36.0	36.0	36.0	32.0	36.0
30	33.96992526858635	36.0	36.0	36.0	32.0	36.0
31	33.99180849587307	36.0	36.0	36.0	32.0	36.0
32	33.94724506525445	36.0	36.0	36.0	32.0	36.0
33	33.912055048816136	36.0	36.0	36.0	32.0	36.0
34	33.909761709584096	36.0	36.0	36.0	32.0	36.0
35	33.88251814069284	36.0	36.0	36.0	32.0	36.0
36	33.830777046222444	36.0	36.0	36.0	32.0	36.0
37	33.83156155257439	36.0	36.0	36.0	32.0	36.0
38	33.23910094596177	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	1.0
7	2.0
8	4.0
9	10.0
10	18.0
11	18.0
12	7.0
13	13.0
14	221.0
15	440.0
16	646.0
17	762.0
18	874.0
19	1113.0
20	1477.0
21	2309.0
22	3336.0
23	5221.0
24	7991.0
25	12345.0
26	18087.0
27	26283.0
28	36763.0
29	49593.0
30	66977.0
31	90424.0
32	123939.0
33	186409.0
34	411769.0
35	797419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.15553654517149	17.2820675384906	11.44361189577195	26.118784020565954
2	16.794832292530085	19.975936397446524	37.35999062626122	25.86924068376217
3	18.511142222892175	24.10491875812663	28.89115660275256	28.49278241622864
4	12.650623639473762	15.43118007606748	36.220590268459105	35.69760601599965
5	14.705386434129753	36.48121652488226	33.6413717350324	15.172025305955597
6	33.84804558079569	35.72214321690727	17.012558140291393	13.41725306200565
7	29.812179210190887	30.553313117961732	21.46192593974099	18.172581732106387
8	27.95482700945803	32.755514471676065	19.86245334012844	19.427205178737466
9	27.84022276342013	13.958821730710428	18.597774534445705	39.60318097142374
10	15.86651507783024	26.48581185960358	31.81744496907772	25.830228093488454
11	37.397475211880945	21.072720490644276	22.45574789420266	19.07405640327212
12	24.769019654406453	23.750813941068767	28.835987011642132	22.644179392882645
13	29.57849199756207	19.38679787527085	25.122709832186306	25.912000294980768
14	23.798545235687545	19.615298069441543	25.229668950297814	31.3564877445731
15	25.124876289638152	26.875611773159257	22.391399248925584	25.608112688277
16	25.765406821642205	25.548516251610415	23.662761190468963	25.023315736278416
17	24.175536948254386	25.633175151389935	25.195666780002373	24.995621120353302
18	25.07415815381846	24.58889734104184	26.03779395554794	24.299150549591758
19	25.75797172957117	24.811774465524067	25.35397678289677	24.076277022007993
20	25.935679410959388	24.047485435287676	24.89650853550428	25.120326618248654
21	27.080721767984556	24.082337504608645	24.375121993537054	24.461818733869745
22	25.98817674267797	24.110097374061382	24.817454133775335	25.084271749485314
23	24.51226356013306	23.87407586137717	25.488522652410012	26.125137926079756
24	24.921361267502913	24.798272198191835	25.192699462583363	25.087667071721885
25	25.128582482849794	24.20869919805742	25.15564106332087	25.50707725577191
26	24.87754248799827	25.08864714612856	25.527232934712647	24.506577431160522
27	25.480069575929505	24.574530611890804	24.685574489404203	25.25982532277549
28	24.553069899585765	24.557244789520485	25.599123815307205	25.290561495586545
29	24.73918020084792	24.80430042927298	25.350366890945576	25.106152478933524
30	24.61921406802938	24.971196735648114	25.62685391363846	24.782735282684047
31	25.21374634764707	24.699800852220203	24.595699644158213	25.490753155974517
32	24.901238062086367	24.794044896423316	24.480869517126507	25.823847524363806
33	24.585090307143513	24.24875983900815	25.259106758762183	25.907043095086145
34	25.31061474445383	24.53902570481378	25.247987524440884	24.902372026291502
35	25.96814713634937	24.35549491992862	25.252973293633385	24.423384650088632
36	24.797742963298752	25.046958336134928	24.828000182194007	25.327298518372316
37	25.665687972911055	24.839681287429613	24.816908357936228	24.677722381723097
38	24.792352431831034	24.622590819209574	25.332705108734725	25.252351640224663
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	436.0
1	358.0
2	280.0
3	280.0
4	698.0
5	1116.0
6	1116.0
7	1241.0
8	1366.0
9	1338.5
10	1311.0
11	1311.0
12	1770.5
13	2230.0
14	2986.0
15	3742.0
16	3742.0
17	5719.0
18	7696.0
19	7696.0
20	9468.0
21	11240.0
22	12351.5
23	13463.0
24	13463.0
25	15859.5
26	18256.0
27	18256.0
28	22928.5
29	27601.0
30	33936.5
31	40272.0
32	40272.0
33	50651.5
34	61031.0
35	61031.0
36	68857.5
37	76684.0
38	87709.5
39	98735.0
40	98735.0
41	106891.5
42	115048.0
43	130183.5
44	145319.0
45	145319.0
46	149314.5
47	153310.0
48	153310.0
49	163163.0
50	173016.0
51	175434.0
52	177852.0
53	177852.0
54	169448.5
55	161045.0
56	161045.0
57	155633.5
58	150222.0
59	134457.5
60	118693.0
61	118693.0
62	111027.0
63	103361.0
64	85449.5
65	67538.0
66	67538.0
67	56205.5
68	44873.0
69	44873.0
70	36054.0
71	27235.0
72	21052.0
73	14869.0
74	14869.0
75	11217.5
76	7566.0
77	7566.0
78	7194.5
79	6823.0
80	5370.0
81	3917.0
82	3917.0
83	3951.0
84	3985.0
85	3985.0
86	2637.5
87	1290.0
88	1164.0
89	1038.0
90	1038.0
91	763.5
92	489.0
93	446.5
94	404.0
95	404.0
96	353.0
97	302.0
98	302.0
99	560.0
100	818.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1194921907190784
2	0.05551724287492573
3	0.012252828993880091
4	0.003090315277217545
5	2.7108028747522325E-4
6	5.963766324454912E-4
7	5.421605749504466E-5
8	3.795124024653126E-4
9	2.1686422998017863E-4
10	4.879445174554019E-4
11	0.002168642299801786
12	0.0027650189322472774
13	0.015397360328592681
14	0.01013840275157335
15	0.024234577700284962
16	0.012144396878890002
17	0.021306910595552547
18	0.005584253921989599
19	0.007698680164296341
20	0.0063432787269202245
21	0.006072198439445001
22	0.007644464106801295
23	0.01013840275157335
24	0.015126280041117459
25	0.017620218685889512
26	0.01968042887070121
27	0.008457704969226966
28	0.006072198439445001
29	0.010355266981553528
30	0.0034156116221878132
31	0.006126414496940046
32	0.007481815934316162
33	0.008403488911731921
34	0.012252828993880091
35	0.01653589753598862
36	0.015397360328592681
37	0.009596242176622903
38	0.006560142956900402
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1844472.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.082326290972674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.51156207001297	46.52869582567353
2	11.332550269280825	12.937766643598767
3	3.1312404779538507	5.362154719741888
4	1.2859104581482408	2.936110414119682
5	0.6685513291509707	1.908123255642959
6	0.4461663599530211	1.528092824333635
7	0.29893204124445255	1.194461542199964
8	0.21266312433460463	0.9711444682660475
9	0.16097217213774617	0.8269799448360103
>10	0.8132596169717158	8.240908540369855
>50	0.060349275350451786	2.427068389497724
>100	0.06467905761430119	8.047455022440591
>500	0.009348168760822865	3.6931073732376625
>1k	0.003815579086050149	3.3979310360416237
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4212	0.22835803416912806	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2821	0.15294349819352096	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2229	0.12084759215645452	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2069	0.11217302295724739	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2010	0.10897427556503976	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1929	0.10458277490794114	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1859	0.10078765088328802	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	5.421605749504465E-5
6	0.0	0.0	0.0	0.0	5.421605749504465E-5
7	0.0	0.0	0.0	0.0	5.421605749504465E-5
8	0.0	5.421605749504465E-5	0.0	0.0	1.084321149900893E-4
9	0.0	5.421605749504465E-5	0.0	0.0	1.084321149900893E-4
10	0.0	5.421605749504465E-5	0.0	0.0	1.6264817248513397E-4
11	0.0	5.421605749504465E-5	0.0	5.421605749504465E-5	2.168642299801786E-4
12	0.0	5.421605749504465E-5	0.0	5.421605749504465E-5	5.963766324454912E-4
13	0.0	5.421605749504465E-5	0.0	5.421605749504465E-5	5.963766324454912E-4
14	0.0	5.421605749504465E-5	0.0	5.421605749504465E-5	5.963766324454912E-4
15	0.0	5.421605749504465E-5	0.0	5.421605749504465E-5	8.132408624256697E-4
16	0.0	5.421605749504465E-5	0.0	5.421605749504465E-5	8.674569199207144E-4
17	0.0	5.421605749504465E-5	0.0	5.421605749504465E-5	8.674569199207144E-4
18	0.0	5.421605749504465E-5	0.0	1.084321149900893E-4	8.674569199207144E-4
19	0.0	5.421605749504465E-5	0.0	1.084321149900893E-4	8.674569199207144E-4
20	0.0	5.421605749504465E-5	0.0	1.084321149900893E-4	9.216729774157591E-4
21	0.0	5.421605749504465E-5	0.0	1.084321149900893E-4	9.216729774157591E-4
22	0.0	5.421605749504465E-5	0.0	1.6264817248513397E-4	0.001084321149900893
23	0.0	5.421605749504465E-5	0.0	2.7108028747522325E-4	0.001084321149900893
24	0.0	5.421605749504465E-5	0.0	8.132408624256697E-4	0.0011385372073959377
25	0.0	5.421605749504465E-5	0.0	9.216729774157591E-4	0.0011385372073959377
26	0.0	5.421605749504465E-5	0.0	0.0011385372073959377	0.0011927532648909824
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTA	40	0.004483742	20.000576	9
CTAGCGG	445	0.0	17.976585	29
TCTAGCG	455	0.0	17.581495	28
GTATCAA	3130	0.0	16.831612	1
TAGCGGC	500	0.0	15.999159	30
CATCGTT	500	0.0	15.999159	28
TACGCTG	70	3.7055044E-4	15.99569	5
CGGCATC	515	0.0	15.534007	25
TAGACCC	135	7.941708E-9	15.404092	4
CGCGGTC	260	0.0	15.385059	10
GTATTAG	360	0.0	15.12344	1
ATCGTTT	530	0.0	15.093547	29
AGCGGCG	520	0.0	15.076131	31
CGTTATT	320	0.0	15.004094	2
TCGTTTA	535	0.0	14.952485	30
CTACGCT	65	0.004168806	14.766052	4
GCATCGT	565	0.0	14.724891	27
CGGTCCA	560	0.0	14.571849	10
AATAGCG	395	0.0	14.173396	5
GTTATTC	350	0.0	14.168763	3
>>END_MODULE