FastQCFastQC Report
Thu 2 Feb 2017
SRR4063011_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063011_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844472
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT47110.25541184685915536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33040.17912985396362754No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22490.12193191330635543No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20750.11249831930221765No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19850.10761887412766363No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19670.10664298509275283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT19640.10648033692026769No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19100.10355266981553529No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19010.10306472529807989No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18740.10160089174571368No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18530.10046235453831776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG550.001363752417.4570625
ACGATTA703.6992002E-415.99926823
GTATCAA33700.015.4316531
CTAGCGG4800.015.33263129
TAGCGGC4850.015.17456330
TCTAGCG4900.015.01972128
GTATTAT2350.014.9800691
CGATTAA756.2490563E-414.93265124
CTAGGAC2800.014.8568663
ATCGTTT3850.014.54478829
TCTAGAT4450.014.3817552
ACGAACG2700.014.22157115
TAACGAA2600.014.15319913
CATCGTT3900.013.94808128
CTAGATA4500.013.8664083
TAGGACT2550.013.8040384
CGAACGA2700.013.62900516
ACCGTCG4050.013.4340338
CGTTATT2750.013.381572
CGTGCAA1208.709931E-613.3348929
Produced by FastQC (version 0.11.5)