FastQCFastQC Report
Thu 2 Feb 2017
SRR4063010_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063010_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146578
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT19201.309882792779271No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15251.0404016973897856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8560.5839894117807584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT8460.5771671055683664No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA7750.5287287314603829No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7480.5103085046869243No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC7360.502121737232054No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.4830192798373562No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4400.30018147334524964No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA3960.2701633260107247No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA3910.26675217290452863No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA3700.25242532985850535No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT3600.24560302364611333No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA3190.21763156817530596No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.21080926196291394No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.20876257009919633No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG2960.2019402638868043No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC2730.18624895959830262No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA2700.184202267734585No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.17669773090095375No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2490.16987542468856173No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG2420.1650998103398873No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG2390.16305311847616968No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT2350.16032419599121286No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT2350.16032419599121286No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT2280.15554858164253843No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC2140.1459973529451896No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT2140.1459973529451896No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA2100.14326843046023277No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC2080.1419039692177544No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA2040.13917504673279757No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA2040.13917504673279757No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT2030.13849281611155834No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA2010.13712835486907995No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC1960.13371720176288393No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA1950.13303497114164473No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC1880.1282593567929703No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG1810.12348374244429587No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG1810.12348374244429587No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT1800.12280151182305667No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT1770.12075481995933905No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA1750.11939035871686066No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG1740.11870812809562145No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT1740.11870812809562145No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC1690.11529697498942543No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT1650.11256805250446861No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1640.1118858218832294No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG1630.11120359126199021No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA1610.10983913001951179No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC1600.1091568993982726No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA1600.1091568993982726No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG1580.10779243815579417No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG1580.10779243815579417No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA1570.10711020753455498No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTA1550.10574574629207657No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA1510.10301682380711977No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT1500.10233459318588056No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1500.10233459318588056No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT1470.10028790132216295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTGC200.003743511431.9989788
TAAGCAA200.003743511431.99897818
CATTCCG356.394441E-527.4276949
AACAGAG356.394441E-527.42769426
TAGAAAT451.2288079E-524.8880924
CCTTTAG401.5773377E-424.015621
AGGCATT401.5846646E-423.9992336
CGGTGTT401.5846646E-423.99923314
GTGACTA1150.023.65141724
GTTAACA350.00205838522.85641323
TTCCTCG350.00205838522.85641328
TGCCCAG1200.022.66594318
TGACTAA1200.022.66594325
AGAACAT854.2200554E-1022.5875135
AGTGACT1250.021.75930423
CAGTGAC1250.021.75930422
ACTAAAG1250.021.75930427
CCAGTGA1250.021.75930421
CTGTAGA908.5310603E-1021.3472181
ATGTCCA604.675616E-621.3326519
Produced by FastQC (version 0.11.5)