FastQCFastQC Report
Thu 2 Feb 2017
SRR4063010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146578
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28201.9238903518945545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23501.6032419599121286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13270.9053200343844233No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10050.6856417743453997No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA8920.6085497141453697No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC7570.5164485802780773No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA6340.43253421386565516No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5230.3568066149081035No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5180.3533954618019075No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4330.2954058589965752No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT3990.27221001787444227No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA3760.2565187135859406No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3660.24969640737354856No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT3500.2387807174337213No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG3470.2367340255700037No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA3070.20944480072043553No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA3040.20739810885671792No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA3030.2067158782354787No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA2990.20398695575052192No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2660.1814733452496282No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC2560.17465103903723617No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG2530.17260434717351855No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG2450.1671465022036049No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2380.16237088785493048No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT2370.16168865723369127No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT2260.15418412040006005No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT2250.15350188977882084No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG2240.1528196591575816No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA2150.1466795835664288No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC2140.1459973529451896No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA2100.14326843046023277No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC2060.14053950797527595No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT2020.13781058549031916No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC2000.13644612424784072No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA1940.13235274052040552No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA1910.1303060486566879No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA1910.1303060486566879No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT1900.1296238180354487No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG1900.1296238180354487No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT1900.1296238180354487No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1900.1296238180354487No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA1890.1289415874142095No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC1840.1255304343080135No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.1255304343080135No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT1810.12348374244429587No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC1810.12348374244429587No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC1800.12280151182305667No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA1790.12211928120181746No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG1790.12211928120181746No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA1750.11939035871686066No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG1730.11802589747438223No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA1710.11666143623190384No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC1710.11666143623190384No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT1700.11597920561066462No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1660.11325028312570783No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG1660.11325028312570783No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC1650.11256805250446861No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1640.1118858218832294No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT1610.10983913001951179No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA1600.1091568993982726No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC1560.10642797691331578No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT1550.10574574629207657No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG1510.10301682380711977No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA1490.10165236256464134No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG1480.10097013194340215No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA1470.10028790132216295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC701.0968506E-925.149564
GATATAC851.6370905E-1124.4687631
CTAAATA401.5814549E-424.00644
TTTAGAA502.7777629E-522.3983272
ATATACA1101.8189894E-1221.8165532
TTAGAAA453.5101396E-421.339023
ATTTAGA604.677344E-621.3317411
CCAATAA453.5182486E-421.331741
CGTGCAA555.7770554E-520.3690669
GACCGTG555.7770554E-520.3690666
CCGTGCA555.7770554E-520.3690668
CTGACCG555.7770554E-520.3690664
TGACCGT555.7770554E-520.3690665
ACTGTTC1102.3646862E-1120.3690668
ACCGTGC555.7770554E-520.3690667
TAGAAAT400.004458269620.0053334
TGGATTG400.004458269620.0053335
TAAATAT400.004458269620.0053335
CTGTTCT1154.3655746E-1119.4834569
ACGTTTT751.4913767E-619.19856629
Produced by FastQC (version 0.11.5)