##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063010_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	146578
Sequences flagged as poor quality	0
Sequence length	38
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.747151687156325	32.0	32.0	32.0	32.0	32.0
2	31.207875670291585	32.0	32.0	32.0	32.0	32.0
3	31.31874496854917	32.0	32.0	32.0	32.0	32.0
4	31.43588396621594	32.0	32.0	32.0	32.0	32.0
5	31.285977431811048	32.0	32.0	32.0	32.0	32.0
6	34.73725934314836	36.0	36.0	36.0	32.0	36.0
7	34.80864113304862	36.0	36.0	36.0	32.0	36.0
8	34.77877307645076	36.0	36.0	36.0	32.0	36.0
9	34.8783855694579	36.0	36.0	36.0	32.0	36.0
10	34.72474723355483	36.0	36.0	36.0	32.0	36.0
11	34.91368418180082	36.0	36.0	36.0	36.0	36.0
12	34.824605329585616	36.0	36.0	36.0	32.0	36.0
13	34.880657397426624	36.0	36.0	36.0	32.0	36.0
14	34.82844628798319	36.0	36.0	36.0	32.0	36.0
15	34.790193617050306	36.0	36.0	36.0	32.0	36.0
16	34.79528305748475	36.0	36.0	36.0	32.0	36.0
17	34.76279523530134	36.0	36.0	36.0	32.0	36.0
18	34.75919987992741	36.0	36.0	36.0	32.0	36.0
19	34.70115569867238	36.0	36.0	36.0	32.0	36.0
20	34.64051904105664	36.0	36.0	36.0	32.0	36.0
21	34.629726152628635	36.0	36.0	36.0	32.0	36.0
22	34.62128695984391	36.0	36.0	36.0	32.0	36.0
23	34.61889915266957	36.0	36.0	36.0	32.0	36.0
24	34.59322681439234	36.0	36.0	36.0	32.0	36.0
25	34.6059231262536	36.0	36.0	36.0	32.0	36.0
26	34.53463684864032	36.0	36.0	36.0	32.0	36.0
27	34.505853538730236	36.0	36.0	36.0	32.0	36.0
28	34.48413813805619	36.0	36.0	36.0	32.0	36.0
29	34.39531171117084	36.0	36.0	36.0	32.0	36.0
30	34.3667126035285	36.0	36.0	36.0	32.0	36.0
31	34.32484411030305	36.0	36.0	36.0	32.0	36.0
32	34.24318792724693	36.0	36.0	36.0	32.0	36.0
33	34.17337526777552	36.0	36.0	36.0	32.0	36.0
34	34.05425780130715	36.0	36.0	36.0	32.0	36.0
35	33.9418057280083	36.0	36.0	36.0	32.0	36.0
36	33.85903750903955	36.0	36.0	36.0	32.0	36.0
37	33.86008132189005	36.0	36.0	36.0	32.0	36.0
38	33.438858491724545	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	5.0
21	3.0
22	8.0
23	33.0
24	110.0
25	248.0
26	613.0
27	1175.0
28	2024.0
29	3273.0
30	4872.0
31	7175.0
32	10141.0
33	15809.0
34	34900.0
35	66187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.4894923580786	22.783842794759828	14.531932314410481	21.194732532751093
2	11.785533981907244	24.807269849499924	42.77313102921311	20.634065139379715
3	16.625960239599394	32.02049420786203	32.210154320566524	19.143391231972057
4	8.85950728968392	20.062492751250197	41.44920417255095	29.62879578651494
5	8.714131725088349	43.435576962436386	35.55581328712358	12.29447802535169
6	24.427267393469688	42.906848230975996	20.243146993409653	12.42273738214466
7	22.634674162209365	36.808890950769566	23.90978059163847	16.6466542953826
8	24.979362382911372	36.49208265962599	21.724417882751045	16.804137074711583
9	26.834277137204065	15.063792044756772	23.135020809169678	34.96691000886948
10	14.624977827504809	30.015418412040006	35.41459154852706	19.94501221192812
11	29.930682531452117	25.481674535381927	27.136151515978497	17.451491417187455
12	22.123374585544898	29.97789572787185	32.733425207056996	15.16530447952626
13	27.31025072488487	24.356131673204846	29.785434078117007	18.54818352379328
14	20.678410129760262	25.141562853907136	31.54634392610078	22.63368309023182
15	21.581683472280968	31.901786079766403	29.563099510158413	16.953430937794213
16	18.772257774017927	29.964251115447066	31.747601959366346	19.51588915116866
17	16.666894076873746	29.6429204928434	32.66451991431183	21.025665515971017
18	17.055765530980093	27.475473809166452	37.793529724788165	17.67523093506529
19	20.068495954372416	28.20614280451364	34.3694142367886	17.35594700432534
20	19.78264132407319	28.339177775655283	35.60356943061032	16.274611469661203
21	21.7680809403931	25.991799531986604	32.6203974702716	19.6197220573487
22	19.388312024996928	29.41437323472827	33.160501575952736	18.03681316432207
23	18.929853047524183	29.02823070310688	34.03102784865396	18.01088840071498
24	18.935310892494098	30.24601236201886	33.79838720681139	17.020289538675655
25	19.66679629136904	28.639554636812054	34.35054612933816	17.343102942480744
26	16.351703529861233	29.14830329244498	36.71424088198775	17.78575229570604
27	18.029308627488437	30.761096481054455	34.526327279673616	16.683267611783485
28	17.113072903164188	29.15307890679365	35.480767918787265	18.253080271254895
29	17.731856076628144	28.928625032405954	35.07074731542251	18.268771575543397
30	18.520514674780664	28.83584166791742	34.902918582597664	17.74072507470425
31	17.984281406486648	28.38011161292964	34.797172836305585	18.838434144278132
32	15.851628484492897	30.94598097941028	35.019580018829565	18.182810517267257
33	17.22018311069874	30.70651803135532	34.80399514251798	17.269303715427963
34	16.748079520801213	30.14026661572678	36.48569362387261	16.625960239599394
35	18.362237170653167	31.055820109429792	33.582120099878566	16.999822620038476
36	17.22973433939609	30.6191925118367	33.93824448416542	18.212828664601783
37	18.597606734980694	30.67240650029336	32.32408683431347	18.405899930412474
38	17.903218103795275	31.81877102137443	32.854404169822004	17.42360670500829
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	403.0
1	276.0
2	149.0
3	149.0
4	529.0
5	909.0
6	909.0
7	1047.5
8	1186.0
9	1122.0
10	1058.0
11	1058.0
12	1305.5
13	1553.0
14	1865.0
15	2177.0
16	2177.0
17	3158.5
18	4140.0
19	4140.0
20	4318.0
21	4496.0
22	4627.5
23	4759.0
24	4759.0
25	4946.0
26	5133.0
27	5133.0
28	6631.5
29	8130.0
30	9482.0
31	10834.0
32	10834.0
33	11759.5
34	12685.0
35	12685.0
36	12805.0
37	12925.0
38	13351.5
39	13778.0
40	13778.0
41	13065.0
42	12352.0
43	11893.5
44	11435.0
45	11435.0
46	12051.5
47	12668.0
48	12668.0
49	11126.5
50	9585.0
51	7287.5
52	4990.0
53	4990.0
54	4257.5
55	3525.0
56	3525.0
57	2946.5
58	2368.0
59	1964.0
60	1560.0
61	1560.0
62	1380.0
63	1200.0
64	1051.0
65	902.0
66	902.0
67	779.5
68	657.0
69	657.0
70	560.5
71	464.0
72	352.0
73	240.0
74	240.0
75	194.0
76	148.0
77	148.0
78	116.5
79	85.0
80	62.0
81	39.0
82	39.0
83	34.5
84	30.0
85	30.0
86	18.0
87	6.0
88	4.0
89	2.0
90	2.0
91	1.5
92	1.0
93	2.0
94	3.0
95	3.0
96	2.5
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012280151182305667
2	0.0
3	0.0
4	6.822306212392038E-4
5	0.0
6	0.0
7	0.0013644612424784076
8	6.822306212392038E-4
9	0.00545784496991363
10	0.0
11	0.004093383727435222
12	0.0
13	0.002046691863717611
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	6.822306212392038E-4
22	0.0
23	0.0
24	0.0
25	6.822306212392038E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.822306212392038E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	146578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.06141440052396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.90054330669413	31.987064907421303
2	11.711831458620631	9.618087298230293
3	3.6353365344675757	4.478161797814133
4	1.6830877099705919	2.764398477261253
5	0.9021881801717979	1.8522561366644381
6	0.6513034376194194	1.6046064211546072
7	0.43032548556997363	1.2368841163066764
8	0.3638659511190124	1.195268048411085
9	0.2674996261651187	0.988552170175606
>10	1.993786033528835	16.771957592544585
>50	0.27580706797148885	7.9111462838898055
>100	0.169471812849951	12.205788044590593
>500	0.008307441806370146	2.267734584999113
>1k	0.006645953445096117	5.118094120536506
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2820	1.9238903518945545	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2350	1.6032419599121286	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1327	0.9053200343844233	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1005	0.6856417743453997	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	892	0.6085497141453697	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	757	0.5164485802780773	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	634	0.43253421386565516	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	523	0.3568066149081035	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	518	0.3533954618019075	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	433	0.2954058589965752	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	399	0.27221001787444227	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	376	0.2565187135859406	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	366	0.24969640737354856	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	350	0.2387807174337213	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	347	0.2367340255700037	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	307	0.20944480072043553	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	304	0.20739810885671792	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	303	0.2067158782354787	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	299	0.20398695575052192	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	266	0.1814733452496282	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC	256	0.17465103903723617	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	253	0.17260434717351855	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG	245	0.1671465022036049	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	238	0.16237088785493048	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT	237	0.16168865723369127	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT	226	0.15418412040006005	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT	225	0.15350188977882084	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	224	0.1528196591575816	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	215	0.1466795835664288	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	214	0.1459973529451896	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA	210	0.14326843046023277	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	206	0.14053950797527595	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT	202	0.13781058549031916	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	200	0.13644612424784072	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	194	0.13235274052040552	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	191	0.1303060486566879	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA	191	0.1303060486566879	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT	190	0.1296238180354487	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG	190	0.1296238180354487	No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT	190	0.1296238180354487	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	190	0.1296238180354487	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA	189	0.1289415874142095	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC	184	0.1255304343080135	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	184	0.1255304343080135	No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT	181	0.12348374244429587	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC	181	0.12348374244429587	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC	180	0.12280151182305667	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	179	0.12211928120181746	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG	179	0.12211928120181746	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	175	0.11939035871686066	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG	173	0.11802589747438223	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	171	0.11666143623190384	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC	171	0.11666143623190384	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT	170	0.11597920561066462	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	166	0.11325028312570783	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG	166	0.11325028312570783	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC	165	0.11256805250446861	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	164	0.1118858218832294	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT	161	0.10983913001951179	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA	160	0.1091568993982726	No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC	156	0.10642797691331578	No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT	155	0.10574574629207657	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	151	0.10301682380711977	No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA	149	0.10165236256464134	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	148	0.10097013194340215	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	147	0.10028790132216295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.822306212392037E-4	0.0
2	0.0	0.0	0.0	6.822306212392037E-4	0.0
3	0.0	0.0	0.0	6.822306212392037E-4	0.0
4	0.0	0.0	0.0	6.822306212392037E-4	0.0
5	0.0	0.0	0.0	6.822306212392037E-4	0.0
6	0.0	0.0	0.0	6.822306212392037E-4	0.0
7	0.0	0.0	0.0	6.822306212392037E-4	0.0
8	0.0	0.0	0.0	6.822306212392037E-4	0.0
9	0.0	0.0	0.0	6.822306212392037E-4	0.0
10	0.0	0.0	0.0	6.822306212392037E-4	0.0
11	0.0	0.0	0.0	6.822306212392037E-4	0.0
12	0.0	0.0	0.0	6.822306212392037E-4	0.0
13	0.0	0.0	0.0	6.822306212392037E-4	0.0
14	0.0	0.0	0.0	6.822306212392037E-4	0.0
15	0.0	0.0	0.0	6.822306212392037E-4	0.0
16	0.0	0.0	0.0	6.822306212392037E-4	0.0
17	0.0	0.0	0.0	0.0013644612424784073	0.0
18	0.0	0.0	0.0	0.0013644612424784073	0.0
19	0.0	0.0	0.0	0.002046691863717611	0.0
20	0.0	0.0	0.0	0.002046691863717611	0.0
21	0.0	0.0	0.0	0.002046691863717611	0.0
22	0.0	0.0	0.0	0.0034111531061960187	0.0
23	0.0	0.0	0.0	0.005457844969913629	0.0
24	0.0	0.0	0.0	0.008186767454870444	0.0
25	0.0	0.0	0.0	0.015009073667262481	0.0
26	0.0	0.0	0.0	0.019784688015936906	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	70	1.0968506E-9	25.14956	4
GATATAC	85	1.6370905E-11	24.468763	1
CTAAATA	40	1.5814549E-4	24.0064	4
TTTAGAA	50	2.7777629E-5	22.398327	2
ATATACA	110	1.8189894E-12	21.816553	2
TTAGAAA	45	3.5101396E-4	21.33902	3
ATTTAGA	60	4.677344E-6	21.331741	1
CCAATAA	45	3.5182486E-4	21.33174	1
CGTGCAA	55	5.7770554E-5	20.369066	9
GACCGTG	55	5.7770554E-5	20.369066	6
CCGTGCA	55	5.7770554E-5	20.369066	8
CTGACCG	55	5.7770554E-5	20.369066	4
TGACCGT	55	5.7770554E-5	20.369066	5
ACTGTTC	110	2.3646862E-11	20.369066	8
ACCGTGC	55	5.7770554E-5	20.369066	7
TAGAAAT	40	0.0044582696	20.005333	4
TGGATTG	40	0.0044582696	20.005333	5
TAAATAT	40	0.0044582696	20.005333	5
CTGTTCT	115	4.3655746E-11	19.483456	9
ACGTTTT	75	1.4913767E-6	19.198566	29
>>END_MODULE