FastQCFastQC Report
Thu 2 Feb 2017
SRR4063009_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063009_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646192
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36740.22318174307735672No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT28100.1706969782382614No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26920.1635289200773664No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24450.14852459494396764No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT24420.14834235617716524No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA23430.14232847687268557No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21880.13291280725456084No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21870.13285206099896002No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21710.13188012090934717No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21650.1315156433757423No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21110.12823534557329885No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC20900.12695967420568197No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC20180.12258594380242403No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG20070.12191773499081517No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG19420.11796922837676285No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG18890.11474967682992021No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18460.11213758783908559No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC17830.10831057373623489No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17610.1069741561130172No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT17460.10606296227900514No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA17290.10503027593379145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT1001.4551915E-1020.7950444
TATAACG400.00449047619.9952355
TCGACGC751.510185E-619.19892314
TCGTTTA4150.018.89050730
TCTAGCG4550.018.63575428
CTAGCGG4650.018.57960329
ATCGTTT4300.018.23153729
TAGCGGC4800.017.99899130
TAGTACG1101.05574145E-817.4657732
ACCGTCG5300.017.2065838
TACCGTC5350.017.0457747
CGTCGTA5400.016.88794310
CGTTTAT4750.016.84116231
CATCGTT4600.016.6942128
ATACCGT5400.016.5896476
CAAGACG6000.016.5293944
AGCGGCG5450.016.43944531
CCGTCGT5550.016.4315139
TTAGGAC2250.016.353153
GTATCAA29600.016.348741
Produced by FastQC (version 0.11.5)