##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063009_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1646192
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29996865493211	32.0	32.0	32.0	32.0	32.0
2	30.971528837462458	32.0	32.0	32.0	32.0	32.0
3	31.021876549029518	32.0	32.0	32.0	32.0	32.0
4	31.07394641694286	32.0	32.0	32.0	32.0	32.0
5	31.0203427060756	32.0	32.0	32.0	32.0	32.0
6	34.65962779554268	36.0	36.0	36.0	32.0	36.0
7	34.59615707037818	36.0	36.0	36.0	32.0	36.0
8	34.59095901328642	36.0	36.0	36.0	32.0	36.0
9	34.7082569955388	36.0	36.0	36.0	32.0	36.0
10	34.47254087008077	36.0	36.0	36.0	32.0	36.0
11	34.67608395618494	36.0	36.0	36.0	32.0	36.0
12	34.5271019419363	36.0	36.0	36.0	32.0	36.0
13	34.596228143497235	36.0	36.0	36.0	32.0	36.0
14	34.510339620165816	36.0	36.0	36.0	32.0	36.0
15	34.46197405891901	36.0	36.0	36.0	32.0	36.0
16	34.482845864880886	36.0	36.0	36.0	32.0	36.0
17	34.408136474967684	36.0	36.0	36.0	32.0	36.0
18	34.43132818043096	36.0	36.0	36.0	32.0	36.0
19	34.42172601980814	36.0	36.0	36.0	32.0	36.0
20	34.401287942111246	36.0	36.0	36.0	32.0	36.0
21	34.379562651258176	36.0	36.0	36.0	32.0	36.0
22	34.35796674992953	36.0	36.0	36.0	32.0	36.0
23	34.30906115447044	36.0	36.0	36.0	32.0	36.0
24	34.29787776881433	36.0	36.0	36.0	32.0	36.0
25	34.27597752874513	36.0	36.0	36.0	32.0	36.0
26	34.2332461827053	36.0	36.0	36.0	32.0	36.0
27	34.2299403714755	36.0	36.0	36.0	32.0	36.0
28	34.21449077628855	36.0	36.0	36.0	32.0	36.0
29	34.19350658975381	36.0	36.0	36.0	32.0	36.0
30	34.16276594710702	36.0	36.0	36.0	32.0	36.0
31	34.17881875261209	36.0	36.0	36.0	32.0	36.0
32	34.1376333987773	36.0	36.0	36.0	32.0	36.0
33	34.10315868379873	36.0	36.0	36.0	32.0	36.0
34	34.1042721626639	36.0	36.0	36.0	32.0	36.0
35	34.08195520328127	36.0	36.0	36.0	32.0	36.0
36	34.035152643191076	36.0	36.0	36.0	32.0	36.0
37	34.03277078250897	36.0	36.0	36.0	32.0	36.0
38	33.44112351414659	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	5.0
9	2.0
10	13.0
11	14.0
12	7.0
13	5.0
14	182.0
15	394.0
16	547.0
17	632.0
18	742.0
19	953.0
20	1290.0
21	1806.0
22	2662.0
23	3947.0
24	6130.0
25	9338.0
26	13609.0
27	19904.0
28	27917.0
29	38768.0
30	52885.0
31	72572.0
32	100280.0
33	153864.0
34	355526.0
35	782197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.46581886629507	17.2218421108648	11.465253260595224	25.84708576224491
2	16.786916172996406	19.292797975359335	37.838207434500944	26.082078417143318
3	19.022623675668385	23.286304714587132	28.554409153235778	29.136662456508706
4	12.35449417247718	15.106363074171675	36.27759006751495	36.26155268583619
5	14.617215884688816	36.5287946888237	33.63732839910848	15.216661027378994
6	34.53014090153901	35.77568742273803	16.506407238554598	13.187764437168362
7	30.44828941477629	30.323212798514877	20.579507481209653	18.64899030549918
8	28.281234148975788	33.02480216403118	19.248785223063965	19.445178463929068
9	27.366204772107245	13.995095344343397	18.612178052243024	40.02652183130633
10	15.889951615401671	26.33519318910086	31.18039588503115	26.594459310466323
11	38.18588161825721	20.983303020473027	22.004524452576472	18.82629090869329
12	24.582937968287368	23.721113040182047	28.683334163549308	23.012614827981277
13	29.431045554874423	19.053020476333106	25.385172930631505	26.130761038160966
14	23.503367161894115	19.87375800783089	24.856367839275354	31.766506990999638
15	25.079535788066593	27.609247782233563	22.182768258597644	25.128448171102203
16	25.91159502702282	25.766760468914036	23.83385094677087	24.487793557292274
17	24.076499916760742	25.981281495730496	25.09785169802012	24.844366889488644
18	25.174380389207553	24.627212939079556	26.330861653567126	23.867545018145766
19	25.577522869456075	24.762192891970145	25.385551874086467	24.27473236448731
20	25.734534305402246	24.191564238377605	24.949714812670592	25.124186643549557
21	27.367254016323482	24.024555083394336	24.317551538641453	24.290639361640732
22	25.986404062986	24.153443332037323	25.230851477449455	24.629301127527217
23	24.300470099645572	23.91663841694643	25.804144993359795	25.9787464900482
24	24.697912774572973	25.04476097608394	25.289905921450334	24.967420327892757
25	24.762928939438126	24.55678283270147	25.35378390201225	25.32650432584816
26	24.360240137408855	25.68383933079408	25.8552358570525	24.10068467474456
27	25.438459533658513	24.51041798927977	25.037498929252934	25.013623547808784
28	24.332732322630967	24.837435514872585	25.931890583652674	24.897941578843774
29	24.168188017727662	24.852082149919656	25.809303074394823	25.170426757957852
30	24.122224584636882	25.41712480636637	26.05090666099687	24.40974394799988
31	25.174685408316133	25.013334532124983	24.522720949248587	25.289259110310297
32	24.799389587880697	24.84798927890816	24.627225182415902	25.725395950795242
33	24.30489236219956	24.451361905525626	25.4380721181768	25.805673614098012
34	25.28525299287057	24.815992758179704	25.44795093545242	24.450803313497307
35	25.853821837352054	24.436380073672947	25.647615350171304	24.0621827388037
36	24.111927861852678	25.421989570783616	25.164570312761057	25.301512254602653
37	25.653766405291734	25.14733855030166	24.85876701584835	24.340128028558254
38	24.338345983420226	24.801136260085947	25.56293595415108	25.297581802342744
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44.0
1	76.5
2	109.0
3	109.0
4	366.5
5	624.0
6	624.0
7	801.0
8	978.0
9	976.0
10	974.0
11	974.0
12	1357.0
13	1740.0
14	2479.0
15	3218.0
16	3218.0
17	4877.5
18	6537.0
19	6537.0
20	8234.5
21	9932.0
22	11159.5
23	12387.0
24	12387.0
25	15295.5
26	18204.0
27	18204.0
28	22971.0
29	27738.0
30	33669.0
31	39600.0
32	39600.0
33	49794.5
34	59989.0
35	59989.0
36	66569.5
37	73150.0
38	83763.0
39	94376.0
40	94376.0
41	99297.5
42	104219.0
43	117145.5
44	130072.0
45	130072.0
46	130711.0
47	131350.0
48	131350.0
49	138922.0
50	146494.0
51	149772.0
52	153050.0
53	153050.0
54	144777.0
55	136504.0
56	136504.0
57	132099.5
58	127695.0
59	113723.0
60	99751.0
61	99751.0
62	96071.0
63	92391.0
64	77776.5
65	63162.0
66	63162.0
67	53224.0
68	43286.0
69	43286.0
70	35010.5
71	26735.0
72	20838.5
73	14942.0
74	14942.0
75	11398.0
76	7854.0
77	7854.0
78	7505.5
79	7157.0
80	5529.5
81	3902.0
82	3902.0
83	3980.0
84	4058.0
85	4058.0
86	2700.5
87	1343.0
88	1156.5
89	970.0
90	970.0
91	696.0
92	422.0
93	347.0
94	272.0
95	272.0
96	260.5
97	249.0
98	249.0
99	481.5
100	714.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11766549709875884
2	0.05242401858349451
3	0.010509102218939225
4	0.002308357712830581
5	1.822387668024143E-4
6	4.252237892056334E-4
7	6.074625560080477E-5
8	3.0373127800402384E-4
9	6.074625560080477E-5
10	4.252237892056334E-4
11	0.0015794026456209238
12	0.002065372690427362
13	0.014761340110995558
14	0.0089296995733183
15	0.02381253219551547
16	0.01069134098574164
17	0.020289249370668793
18	0.004738207936862772
19	0.006682088116088523
20	0.004920446703665186
21	0.0058923867932780625
22	0.006803580627290135
23	0.009597908384927153
24	0.013789400021382682
25	0.016522981523418898
26	0.018284622935842234
27	0.008808207062116692
28	0.004616715425661162
29	0.010752087241342443
30	0.0025513427352338
31	0.005467163004072429
32	0.0055279092596732336
33	0.007046565649693352
34	0.01196701235335854
35	0.01634074275661648
36	0.01451835508859234
37	0.009294177106923129
38	0.005467163004072429
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1646192.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.51475377892646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.11507423546314	41.547086436768346
2	12.91660013687389	13.566241516975602
3	3.7500902241662755	5.908051943125533
4	1.4857476542383028	3.1209468895976813
5	0.7321738475780948	1.9224964664466437
6	0.4293147086522277	1.352721373112597
7	0.2831234636677261	1.0407711288483228
8	0.1928739548310097	0.8102982598654607
9	0.14054967806666288	0.6642838563640363
>10	0.7792142915885623	7.503702662341413
>50	0.07390057843184746	2.750215298629032
>100	0.08109327729243876	9.296133071084041
>500	0.013495966099910727	4.818069732851775
>1k	0.0067479830499553636	5.698981363989477
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3674	0.22318174307735672	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2810	0.1706969782382614	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2692	0.1635289200773664	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2445	0.14852459494396764	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2442	0.14834235617716524	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2343	0.14232847687268557	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2188	0.13291280725456084	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2187	0.13285206099896002	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2171	0.13188012090934717	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2165	0.1315156433757423	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2111	0.12823534557329885	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2090	0.12695967420568197	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2018	0.12258594380242403	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2007	0.12191773499081517	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1942	0.11796922837676285	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1889	0.11474967682992021	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1846	0.11213758783908559	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1783	0.10831057373623489	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1761	0.1069741561130172	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1746	0.10606296227900514	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1729	0.10503027593379145	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2149251120160953E-4
2	0.0	0.0	0.0	0.0	1.2149251120160953E-4
3	0.0	0.0	0.0	0.0	1.2149251120160953E-4
4	0.0	0.0	0.0	0.0	1.2149251120160953E-4
5	0.0	0.0	0.0	0.0	1.2149251120160953E-4
6	0.0	0.0	0.0	0.0	1.2149251120160953E-4
7	0.0	0.0	0.0	0.0	1.2149251120160953E-4
8	0.0	0.0	0.0	0.0	1.2149251120160953E-4
9	0.0	0.0	0.0	0.0	2.4298502240321907E-4
10	0.0	0.0	0.0	0.0	2.4298502240321907E-4
11	0.0	0.0	0.0	0.0	2.4298502240321907E-4
12	0.0	0.0	0.0	0.0	3.644775336048286E-4
13	0.0	0.0	0.0	0.0	4.2522378920563337E-4
14	0.0	0.0	0.0	0.0	4.2522378920563337E-4
15	0.0	0.0	0.0	6.074625560080477E-5	4.8597004480643814E-4
16	0.0	0.0	0.0	6.074625560080477E-5	4.8597004480643814E-4
17	0.0	0.0	0.0	6.074625560080477E-5	4.8597004480643814E-4
18	0.0	0.0	0.0	6.074625560080477E-5	5.467163004072429E-4
19	0.0	0.0	0.0	6.074625560080477E-5	5.467163004072429E-4
20	0.0	0.0	0.0	6.074625560080477E-5	5.467163004072429E-4
21	0.0	0.0	0.0	6.074625560080477E-5	5.467163004072429E-4
22	0.0	0.0	6.074625560080477E-5	1.822387668024143E-4	5.467163004072429E-4
23	0.0	0.0	6.074625560080477E-5	3.644775336048286E-4	6.074625560080477E-4
24	0.0	0.0	6.074625560080477E-5	7.897013228104619E-4	6.074625560080477E-4
25	0.0	6.074625560080477E-5	6.074625560080477E-5	0.0015794026456209238	6.074625560080477E-4
26	0.0	6.074625560080477E-5	6.074625560080477E-5	0.002126118946028167	6.074625560080477E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACT	100	1.4551915E-10	20.795044	4
TATAACG	40	0.004490476	19.995235	5
TCGACGC	75	1.510185E-6	19.198923	14
TCGTTTA	415	0.0	18.890507	30
TCTAGCG	455	0.0	18.635754	28
CTAGCGG	465	0.0	18.579603	29
ATCGTTT	430	0.0	18.231537	29
TAGCGGC	480	0.0	17.998991	30
TAGTACG	110	1.05574145E-8	17.465773	2
ACCGTCG	530	0.0	17.206583	8
TACCGTC	535	0.0	17.045774	7
CGTCGTA	540	0.0	16.887943	10
CGTTTAT	475	0.0	16.841162	31
CATCGTT	460	0.0	16.69421	28
ATACCGT	540	0.0	16.589647	6
CAAGACG	600	0.0	16.529394	4
AGCGGCG	545	0.0	16.439445	31
CCGTCGT	555	0.0	16.431513	9
TTAGGAC	225	0.0	16.35315	3
GTATCAA	2960	0.0	16.34874	1
>>END_MODULE