FastQCFastQC Report
Thu 2 Feb 2017
SRR4063009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646192
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38720.23520950168631605No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC28260.17166891832787426No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT27730.16844936678103162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27420.16656623285740665No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24840.15089369891239904No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT24430.14840310243276603No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA23920.145305043397125No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC23500.1427537006618912No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC22010.1337025085773713No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC21930.13321653853256485No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21610.1312726583533391No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21270.12920728566291176No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC20200.12270743631362561No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19930.12106728741240391No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG18640.11323102043990009No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18520.11250206537269042No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18520.11250206537269042No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG18370.11159087153867836No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA17590.10685266360181558No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC17020.10339012703256972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4900.019.59077628
CTAGCGG5000.019.19896129
ACCGAAC450.00884377117.7805968
TAGCGGC5350.017.64390630
GAGCGTT1700.016.9443787
GTATCAA28600.016.1119961
GCGTTCC1601.8189894E-1116.001089
TTTCGGA6200.015.48303330
ATAGACC1255.1348252E-815.3615023
GTTTTCG6150.015.34876328
TTTTCGG6300.015.23727129
AGCGGCG6100.015.21229231
TAACCCG2550.015.05800828
ATCGTTT4150.015.03533229
GTATTAT1308.315692E-814.7693281
ACCGTCG5650.014.7279998
CATCGTT4250.014.68155928
TACCGTC5600.014.57418257
TCTAGAT4300.014.5126072
CGGTCCA7200.014.44498110
Produced by FastQC (version 0.11.5)