##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063009_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1646192
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.214519327028682	32.0	32.0	32.0	32.0	32.0
2	31.27305077415028	32.0	32.0	32.0	32.0	32.0
3	31.36159026407612	32.0	32.0	32.0	32.0	32.0
4	31.462346433465843	32.0	32.0	32.0	32.0	32.0
5	31.37573199237999	32.0	32.0	32.0	32.0	32.0
6	34.91341046487894	36.0	36.0	36.0	36.0	36.0
7	34.93904781459271	36.0	36.0	36.0	36.0	36.0
8	34.868128383566436	36.0	36.0	36.0	32.0	36.0
9	35.00213583834692	36.0	36.0	36.0	36.0	36.0
10	34.83021300067064	36.0	36.0	36.0	32.0	36.0
11	35.00504436906509	36.0	36.0	36.0	36.0	36.0
12	34.90216511804213	36.0	36.0	36.0	32.0	36.0
13	34.96133926054798	36.0	36.0	36.0	36.0	36.0
14	34.9022082478836	36.0	36.0	36.0	32.0	36.0
15	34.87669482053126	36.0	36.0	36.0	32.0	36.0
16	34.886742251207636	36.0	36.0	36.0	32.0	36.0
17	34.85413426866368	36.0	36.0	36.0	32.0	36.0
18	34.85472350734301	36.0	36.0	36.0	32.0	36.0
19	34.84907228318446	36.0	36.0	36.0	32.0	36.0
20	34.84282331587081	36.0	36.0	36.0	32.0	36.0
21	34.833155549291945	36.0	36.0	36.0	32.0	36.0
22	34.81371128033668	36.0	36.0	36.0	32.0	36.0
23	34.75573687637894	36.0	36.0	36.0	32.0	36.0
24	34.72299585953522	36.0	36.0	36.0	32.0	36.0
25	34.707888265767295	36.0	36.0	36.0	32.0	36.0
26	34.65061851837453	36.0	36.0	36.0	32.0	36.0
27	34.63805194047839	36.0	36.0	36.0	32.0	36.0
28	34.59478359753905	36.0	36.0	36.0	32.0	36.0
29	34.561922910571795	36.0	36.0	36.0	32.0	36.0
30	34.54876952384655	36.0	36.0	36.0	32.0	36.0
31	34.539053767725754	36.0	36.0	36.0	32.0	36.0
32	34.51237461972844	36.0	36.0	36.0	32.0	36.0
33	34.48662306705415	36.0	36.0	36.0	32.0	36.0
34	34.48066628922993	36.0	36.0	36.0	32.0	36.0
35	34.45345682642122	36.0	36.0	36.0	32.0	36.0
36	34.42038534994703	36.0	36.0	36.0	32.0	36.0
37	34.42591994129482	36.0	36.0	36.0	32.0	36.0
38	33.9818307949498	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	6.0
21	31.0
22	117.0
23	335.0
24	986.0
25	2525.0
26	5545.0
27	10537.0
28	18681.0
29	29686.0
30	45662.0
31	66301.0
32	96407.0
33	155764.0
34	381219.0
35	832389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.85433958229296	18.505389317596112	12.19810769400875	26.442163406102182
2	15.836366596363	20.338332345194242	37.048108604585615	26.777192453857147
3	18.031797020031686	24.03759707251645	28.819603059667404	29.111002847784462
4	11.889332337093531	15.979382695800606	36.1427295755654	35.988555391540466
5	14.066342200666751	37.14250828578927	33.23889315462595	15.552256358918036
6	34.571060795570865	36.235450485940554	16.115464263216435	13.078024455272146
7	30.280163773098934	30.546598792355635	20.529103743211557	18.644133691333874
8	28.116081484478368	33.34135202417271	18.98206364653611	19.56050284481282
9	27.334469733326692	13.753072350124596	18.417334190290706	40.49512372625801
10	15.76082037350783	26.617247112552295	31.18042592017748	26.441506593762398
11	38.30235616804517	20.908022270754294	21.86889657707133	18.920724984129205
12	24.45516418577753	23.880987787513426	28.581616635807418	23.082231390901626
13	29.42121971636255	19.053375499487906	25.375747333533393	26.149657450616154
14	23.399350379147986	19.841561519896537	24.84177109460567	31.917317006349812
15	25.116146840708737	27.598360337068822	21.977266321303954	25.308226500918487
16	25.855064293836925	25.90894622255484	23.615957312391263	24.620032171216966
17	24.194990620778135	26.040036642141377	24.871764654426702	24.893208082653786
18	25.170150261937852	24.673245891123273	26.273909726204476	23.8826941207344
19	25.571622265203576	24.79716825254891	25.22798069726982	24.403228784977692
20	25.790187292855272	24.143720781051055	24.83689630371184	25.229195622381834
21	27.408058967641058	24.109717523665235	24.246639666964526	24.23558384172918
22	25.90278157442337	24.216038245888992	25.20717535642909	24.674004823258556
23	24.257664217170692	23.91657337037242	25.749351988137448	26.07641042431944
24	24.736179011925703	25.072227297909354	25.204957866397116	24.986635823767823
25	24.804640041987813	24.608247397630407	25.359131863111955	25.22798069726982
26	24.505403986898248	25.6945726865396	25.724338351784	24.075684974778156
27	25.521947331749473	24.453055568885993	24.99090324269784	25.034093856666694
28	24.33744059016202	24.805368997055023	25.9741269548145	24.88306345796845
29	24.306520746061214	24.74656662163344	25.745599541244278	25.201313091061067
30	24.16364555288812	25.294923070941906	26.035176941693315	24.50625443447666
31	25.165533546512194	24.84625122707436	24.612378143011266	25.375837083402182
32	24.860708835907356	24.860040627095746	24.70325454139007	25.57599599560683
33	24.322436265028625	24.361192376101936	25.51561421753963	25.80075714132981
34	25.28204486475454	24.70185737751125	25.58802375421579	24.428074003518425
35	25.971514865823668	24.354996258030656	25.63121434194796	24.042274534197713
36	24.26175075568342	25.405784987413377	25.11201609532788	25.22044816157532
37	25.68552149445508	24.983780749754587	24.784411538872746	24.54628621691759
38	24.46549369696852	24.607882920096806	25.54027719731356	25.386346185621118
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	43.0
2	65.0
3	65.0
4	258.0
5	451.0
6	451.0
7	635.0
8	819.0
9	881.0
10	943.0
11	943.0
12	1323.0
13	1703.0
14	2419.0
15	3135.0
16	3135.0
17	4993.5
18	6852.0
19	6852.0
20	8383.5
21	9915.0
22	11240.5
23	12566.0
24	12566.0
25	15450.0
26	18334.0
27	18334.0
28	23069.5
29	27805.0
30	33665.0
31	39525.0
32	39525.0
33	49882.5
34	60240.0
35	60240.0
36	66420.0
37	72600.0
38	83304.5
39	94009.0
40	94009.0
41	99281.5
42	104554.0
43	117720.0
44	130886.0
45	130886.0
46	130946.5
47	131007.0
48	131007.0
49	139002.5
50	146998.0
51	149671.0
52	152344.0
53	152344.0
54	144549.5
55	136755.0
56	136755.0
57	132554.5
58	128354.0
59	114552.5
60	100751.0
61	100751.0
62	96973.5
63	93196.0
64	78198.0
65	63200.0
66	63200.0
67	53597.5
68	43995.0
69	43995.0
70	35360.0
71	26725.0
72	20856.0
73	14987.0
74	14987.0
75	11228.5
76	7470.0
77	7470.0
78	7197.5
79	6925.0
80	5409.0
81	3893.0
82	3893.0
83	3648.5
84	3404.0
85	3404.0
86	2177.5
87	951.0
88	779.5
89	608.0
90	608.0
91	357.5
92	107.0
93	81.0
94	55.0
95	55.0
96	37.5
97	20.0
98	20.0
99	22.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009597908384927153
2	0.0
3	0.0
4	1.2149251120160953E-4
5	0.0
6	1.822387668024143E-4
7	7.289550672096572E-4
8	0.002308357712830581
9	0.003523282824846676
10	9.111938340120716E-4
11	0.005284924237270014
12	4.8597004480643814E-4
13	3.644775336048286E-4
14	6.074625560080477E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	6.074625560080477E-5
22	1.2149251120160953E-4
23	1.822387668024143E-4
24	0.0
25	0.0
26	0.0
27	6.074625560080477E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1646192.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.83100359439382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.95610192667208	39.625868972396
2	13.487504977954835	13.711668280276538
3	3.9913507444851835	6.086530921182385
4	1.5979147457782445	3.2489444074475538
5	0.8062807248491762	2.0492029211449467
6	0.46899458603161465	1.4303679292994547
7	0.2956743117965298	1.052059540398953
8	0.22063451029876863	0.8972058868835224
9	0.15100537577189177	0.6908179318770439
>10	0.8384233529008727	7.751638959078227
>50	0.07970016346910301	2.8731062790206328
>100	0.08462625611227681	9.450213674873659
>500	0.01468605255959462	5.188161004506081
>1k	0.007102271319803956	5.944213291614989
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3872	0.23520950168631605	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2826	0.17166891832787426	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2773	0.16844936678103162	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2742	0.16656623285740665	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2484	0.15089369891239904	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2443	0.14840310243276603	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2392	0.145305043397125	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2350	0.1427537006618912	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2201	0.1337025085773713	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2193	0.13321653853256485	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2161	0.1312726583533391	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2127	0.12920728566291176	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2020	0.12270743631362561	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1993	0.12106728741240391	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1864	0.11323102043990009	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1852	0.11250206537269042	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1852	0.11250206537269042	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1837	0.11159087153867836	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1759	0.10685266360181558	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1702	0.10339012703256972	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	6.074625560080477E-5
6	0.0	0.0	0.0	0.0	1.2149251120160953E-4
7	0.0	0.0	0.0	0.0	1.2149251120160953E-4
8	0.0	0.0	0.0	0.0	1.2149251120160953E-4
9	0.0	0.0	0.0	0.0	1.2149251120160953E-4
10	0.0	0.0	0.0	0.0	1.2149251120160953E-4
11	0.0	0.0	0.0	0.0	1.2149251120160953E-4
12	0.0	0.0	0.0	0.0	1.822387668024143E-4
13	0.0	0.0	0.0	0.0	1.822387668024143E-4
14	0.0	0.0	0.0	0.0	1.822387668024143E-4
15	0.0	0.0	0.0	0.0	3.0373127800402384E-4
16	0.0	0.0	0.0	0.0	3.0373127800402384E-4
17	0.0	0.0	0.0	0.0	3.0373127800402384E-4
18	0.0	0.0	0.0	6.074625560080477E-5	3.0373127800402384E-4
19	0.0	0.0	0.0	6.074625560080477E-5	3.0373127800402384E-4
20	0.0	0.0	0.0	6.074625560080477E-5	4.2522378920563337E-4
21	0.0	0.0	0.0	6.074625560080477E-5	4.2522378920563337E-4
22	0.0	0.0	0.0	1.822387668024143E-4	4.2522378920563337E-4
23	0.0	0.0	0.0	3.644775336048286E-4	4.2522378920563337E-4
24	0.0	0.0	0.0	7.897013228104619E-4	4.8597004480643814E-4
25	0.0	0.0	0.0	0.0013971638788185096	4.8597004480643814E-4
26	0.0	0.0	0.0	0.0016401489012217287	5.467163004072429E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	490	0.0	19.590776	28
CTAGCGG	500	0.0	19.198961	29
ACCGAAC	45	0.008843771	17.780596	8
TAGCGGC	535	0.0	17.643906	30
GAGCGTT	170	0.0	16.944378	7
GTATCAA	2860	0.0	16.111996	1
GCGTTCC	160	1.8189894E-11	16.00108	9
TTTCGGA	620	0.0	15.483033	30
ATAGACC	125	5.1348252E-8	15.361502	3
GTTTTCG	615	0.0	15.348763	28
TTTTCGG	630	0.0	15.237271	29
AGCGGCG	610	0.0	15.212292	31
TAACCCG	255	0.0	15.058008	28
ATCGTTT	415	0.0	15.035332	29
GTATTAT	130	8.315692E-8	14.769328	1
ACCGTCG	565	0.0	14.727999	8
CATCGTT	425	0.0	14.681559	28
TACCGTC	560	0.0	14.5741825	7
TCTAGAT	430	0.0	14.512607	2
CGGTCCA	720	0.0	14.444981	10
>>END_MODULE