FastQCFastQC Report
Thu 2 Feb 2017
SRR4063008_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063008_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062397
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32210.30318233202842254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22760.21423253265963663No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14470.13620143882183403No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13940.1312127199154365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12570.11831735217625802No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12460.11728195768625099No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12450.11718783091443218No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12040.11332863326986051No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11880.11182260492075936No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11850.11154022460530293No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11680.10994006948438295No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11420.10749277341709361No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT11280.10617499861163011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG3300.018.4216145
CGGTCCA3250.018.21535910
TAGCGGC3250.018.21450230
TCTAGCG3300.017.9393728
GAGCGAA3250.017.72221816
CTAGCGG3350.017.67161629
CCGGTCC3550.017.577449
TACACCG652.1025051E-417.2283125
AGACGGA3450.017.1569736
CGCCGGT3550.017.1251227
GCCTAGA854.9387872E-616.9571171
AGCGGCG3500.016.91346531
TCTTGCG3700.016.87042
CAATACT957.702529E-716.83974
ACGAACG1051.1888369E-716.7618815
CGCAAGA3250.016.7439542
AGCGAAA3350.016.71560717
GACGGAC3350.016.714827
GTAGAAC1151.8435458E-816.6932723
CTTGCGC3950.016.6052253
Produced by FastQC (version 0.11.5)