##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063008_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1062397
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238416524143048	32.0	32.0	32.0	32.0	32.0
2	30.861341852433696	32.0	32.0	32.0	32.0	32.0
3	30.92491789792328	32.0	32.0	32.0	32.0	32.0
4	30.98362853057755	32.0	32.0	32.0	32.0	32.0
5	30.912219255137202	32.0	32.0	32.0	32.0	32.0
6	34.56511078250409	36.0	36.0	36.0	32.0	36.0
7	34.49258233974682	36.0	36.0	36.0	32.0	36.0
8	34.45976221694903	36.0	36.0	36.0	32.0	36.0
9	34.60385147924928	36.0	36.0	36.0	32.0	36.0
10	34.33013270933559	36.0	36.0	36.0	32.0	36.0
11	34.598908882461075	36.0	36.0	36.0	32.0	36.0
12	34.420837031731075	36.0	36.0	36.0	32.0	36.0
13	34.50975200419429	36.0	36.0	36.0	32.0	36.0
14	34.4029322371957	36.0	36.0	36.0	32.0	36.0
15	34.35269772034371	36.0	36.0	36.0	32.0	36.0
16	34.35508006893845	36.0	36.0	36.0	32.0	36.0
17	34.29394002430353	36.0	36.0	36.0	32.0	36.0
18	34.32879516790804	36.0	36.0	36.0	32.0	36.0
19	34.31753478219536	36.0	36.0	36.0	32.0	36.0
20	34.295859269180916	36.0	36.0	36.0	32.0	36.0
21	34.26392299677051	36.0	36.0	36.0	32.0	36.0
22	34.253986974737316	36.0	36.0	36.0	32.0	36.0
23	34.19908565253855	36.0	36.0	36.0	32.0	36.0
24	34.19211085874678	36.0	36.0	36.0	32.0	36.0
25	34.165856078283355	36.0	36.0	36.0	32.0	36.0
26	34.11732054966269	36.0	36.0	36.0	32.0	36.0
27	34.12821289969757	36.0	36.0	36.0	32.0	36.0
28	34.1026283018495	36.0	36.0	36.0	32.0	36.0
29	34.07032116995812	36.0	36.0	36.0	32.0	36.0
30	34.0374473949004	36.0	36.0	36.0	32.0	36.0
31	34.06172551315563	36.0	36.0	36.0	32.0	36.0
32	34.01020710713603	36.0	36.0	36.0	32.0	36.0
33	33.976358178722265	36.0	36.0	36.0	32.0	36.0
34	33.974806028254974	36.0	36.0	36.0	32.0	36.0
35	33.93342789936342	36.0	36.0	36.0	32.0	36.0
36	33.89059927691814	36.0	36.0	36.0	32.0	36.0
37	33.88561902942121	36.0	36.0	36.0	32.0	36.0
38	33.298914624194154	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	5.0
10	6.0
11	13.0
12	3.0
13	2.0
14	124.0
15	246.0
16	346.0
17	432.0
18	519.0
19	725.0
20	974.0
21	1353.0
22	1990.0
23	3027.0
24	4506.0
25	6872.0
26	10157.0
27	14365.0
28	20224.0
29	27535.0
30	36973.0
31	49335.0
32	67739.0
33	101646.0
34	227187.0
35	486087.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.98912288538507	17.048571942849442	11.263402024451361	25.698903147314127
2	16.615811164101288	19.484909100498008	37.56347520135012	26.335804534050585
3	18.941387227625185	23.73495801942431	28.46178539119269	28.861869361757808
4	12.342722673255677	14.951782562654431	36.17997411460171	36.52552064948817
5	14.417424780801868	36.768810094174015	33.68436410186418	15.129401023159936
6	34.61609780937939	35.56988797930709	16.626333193083916	13.187681018229599
7	30.345906473756983	30.56748089461849	20.71692597023523	18.36968666138929
8	28.47771820570938	32.87957740645637	19.36140332382021	19.281301064014038
9	27.51001275420159	14.019267786463605	18.588284018655962	39.88243544067884
10	15.715103276380093	26.842446102756796	31.667218879660236	25.77523174120287
11	37.91704827947141	21.222880389918082	21.75611859066979	19.10395273994072
12	24.446119945141472	24.005783301282325	28.8085401588337	22.739556594742506
13	29.678106294965044	19.215054593518822	25.280216604187107	25.826622507329034
14	23.49314114222669	19.622207411256586	25.295591729456373	31.589059717060348
15	25.216243469386022	27.050229118747254	22.672069732693227	25.0614576791735
16	25.686979999209232	25.919503548102252	23.772190936665105	24.621325516023415
17	23.849366476047727	25.97589426768623	25.323069912649203	24.851669343616837
18	24.855671432592228	24.89219482026822	26.385982549727814	23.86615119741173
19	25.327468166281815	25.261103794818307	25.697884906294448	23.713543132605427
20	25.567034570399834	24.22130703880639	25.472807285905912	24.738851104887864
21	26.85234692110158	24.329962120926872	24.62111513581493	24.19657582215662
22	25.48813802478015	24.45745183573033	25.341757805219235	24.712652334270295
23	24.123771510336258	24.25048009940882	25.923014647738825	25.7027337425161
24	24.571957383552736	25.26418751688682	25.516960250193225	24.64689484936722
25	24.782195275798117	24.696523220892683	25.50362929419407	25.01765220911513
26	24.361512730203295	25.578562155006924	25.910245076302136	24.14968003848765
27	25.17749457302459	24.972841588800005	25.0617065460714	24.78795729210401
28	24.21585617370006	24.86066097669572	26.137656882891207	24.78582596671301
29	24.14684499036491	25.338169549348887	25.960802550780738	24.554182909505464
30	24.19530800677358	25.361293644451095	26.212791672902764	24.23060667587256
31	24.69515466770588	25.355394025652757	24.949215977528894	25.00023532911247
32	24.32976628423181	25.213966465722066	25.099028914183723	25.357238335862398
33	24.071143677733524	24.785724573355385	25.65327529579658	25.489856453114513
34	24.82995919020565	25.019369354527438	25.7781396947032	24.372531760563714
35	25.486795813598366	24.77120620296912	25.69626652582703	24.04573145760548
36	24.197046894946734	25.652322905957803	25.289398270012164	24.861231929083303
37	25.253156632812036	25.502147724673275	25.1049857524379	24.139709890076787
38	24.401576530146386	25.155200864512352	25.68206014498279	24.761162460358474
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	72.0
1	109.0
2	146.0
3	146.0
4	404.0
5	662.0
6	662.0
7	881.0
8	1100.0
9	1103.0
10	1106.0
11	1106.0
12	1415.5
13	1725.0
14	2237.5
15	2750.0
16	2750.0
17	4055.5
18	5361.0
19	5361.0
20	6355.0
21	7349.0
22	7928.0
23	8507.0
24	8507.0
25	9928.5
26	11350.0
27	11350.0
28	14520.5
29	17691.0
30	21529.5
31	25368.0
32	25368.0
33	31386.0
34	37404.0
35	37404.0
36	41868.5
37	46333.0
38	53076.0
39	59819.0
40	59819.0
41	63602.0
42	67385.0
43	75869.5
44	84354.0
45	84354.0
46	87339.0
47	90324.0
48	90324.0
49	94132.0
50	97940.0
51	98400.5
52	98861.0
53	98861.0
54	93286.5
55	87712.0
56	87712.0
57	85143.5
58	82575.0
59	73596.5
60	64618.0
61	64618.0
62	61049.0
63	57480.0
64	47764.5
65	38049.0
66	38049.0
67	31919.5
68	25790.0
69	25790.0
70	20578.5
71	15367.0
72	12131.5
73	8896.0
74	8896.0
75	6809.0
76	4722.0
77	4722.0
78	4505.0
79	4288.0
80	3406.0
81	2524.0
82	2524.0
83	2402.0
84	2280.0
85	2280.0
86	1551.5
87	823.0
88	696.0
89	569.0
90	569.0
91	414.5
92	260.0
93	214.0
94	168.0
95	168.0
96	157.0
97	146.0
98	146.0
99	334.5
100	523.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1195410002099027
2	0.05318162607763388
3	0.011295212618258523
4	0.0020707889800140626
5	1.8825354363764205E-4
6	3.765070872752841E-4
7	0.0
8	4.706338590941051E-4
9	1.8825354363764205E-4
10	4.706338590941051E-4
11	0.0018825354363764203
12	0.0026355496109269887
13	0.017225199242844248
14	0.011389339390077344
15	0.02673200319654517
16	0.013083621282816121
17	0.022872805551973508
18	0.006024113396404546
19	0.007530141745505681
20	0.006777127570955114
21	0.00649474725549865
22	0.008942043322787996
23	0.01101283230280206
24	0.016754565383750142
25	0.019484241766495953
26	0.0224021716928794
27	0.010448071671889134
28	0.005647606309129262
29	0.012612987423722018
30	0.0032003102418399145
31	0.005176972450035156
32	0.007624268517324503
33	0.009036170094606819
34	0.014307269316460796
35	0.01844884727648892
36	0.018166466961032458
37	0.010353944900070312
38	0.006024113396404546
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1062397.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.82899281780526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.37596918168532	44.540273897145205
2	13.484337882350076	15.326026813378634
3	4.036813522681252	6.88224140061816
4	1.5893195752154496	3.612777229004643
5	0.8012899560206774	2.276825057783928
6	0.434843547223158	1.482703249320828
7	0.24554534892817048	0.976786640947914
8	0.16552249041078596	0.7525181134991835
9	0.12001604872834072	0.6138352054083817
>10	0.5860868326107844	6.272283389479391
>50	0.07118052699848718	2.8291647099806796
>100	0.07862493819021553	9.322745174244142
>500	0.00812789363356498	3.213410082504055
>1k	0.0023222553238757084	1.8984090366848296
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3221	0.30318233202842254	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2276	0.21423253265963663	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1447	0.13620143882183403	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1394	0.1312127199154365	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1257	0.11831735217625802	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1246	0.11728195768625099	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1245	0.11718783091443218	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1204	0.11332863326986051	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1188	0.11182260492075936	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1185	0.11154022460530293	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1168	0.10994006948438295	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1142	0.10749277341709361	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1128	0.10617499861163011	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8825354363764205E-4
2	0.0	0.0	0.0	0.0	1.8825354363764205E-4
3	0.0	0.0	0.0	0.0	1.8825354363764205E-4
4	0.0	0.0	0.0	0.0	1.8825354363764205E-4
5	0.0	0.0	0.0	0.0	1.8825354363764205E-4
6	0.0	0.0	0.0	0.0	2.823803154564631E-4
7	0.0	0.0	0.0	0.0	2.823803154564631E-4
8	0.0	0.0	0.0	0.0	2.823803154564631E-4
9	0.0	0.0	0.0	0.0	2.823803154564631E-4
10	0.0	0.0	0.0	0.0	3.765070872752841E-4
11	0.0	0.0	0.0	0.0	3.765070872752841E-4
12	0.0	0.0	0.0	0.0	6.588874027317472E-4
13	0.0	0.0	0.0	0.0	7.530141745505682E-4
14	0.0	0.0	0.0	0.0	7.530141745505682E-4
15	0.0	0.0	0.0	9.412677181882103E-5	9.412677181882103E-4
16	0.0	0.0	0.0	1.8825354363764205E-4	0.0012236480336446733
17	0.0	0.0	0.0	2.823803154564631E-4	0.0012236480336446733
18	0.0	0.0	0.0	3.765070872752841E-4	0.0012236480336446733
19	0.0	0.0	0.0	3.765070872752841E-4	0.0012236480336446733
20	0.0	0.0	0.0	5.647606309129262E-4	0.0013177748054634944
21	0.0	0.0	0.0	6.588874027317472E-4	0.0015060283491011364
22	0.0	0.0	0.0	0.0010353944900070313	0.0016001551209199574
23	0.0	0.0	0.0	0.0015060283491011364	0.0016942818927387785
24	0.0	0.0	0.0	0.0023531692954705257	0.0016942818927387785
25	0.0	0.0	0.0	0.002917929926383452	0.0016942818927387785
26	0.0	0.0	0.0	0.003388563785477557	0.0016942818927387785
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	330	0.0	18.421614	5
CGGTCCA	325	0.0	18.215359	10
TAGCGGC	325	0.0	18.214502	30
TCTAGCG	330	0.0	17.93937	28
GAGCGAA	325	0.0	17.722218	16
CTAGCGG	335	0.0	17.671616	29
CCGGTCC	355	0.0	17.57744	9
TACACCG	65	2.1025051E-4	17.228312	5
AGACGGA	345	0.0	17.156973	6
CGCCGGT	355	0.0	17.125122	7
GCCTAGA	85	4.9387872E-6	16.957117	1
AGCGGCG	350	0.0	16.913465	31
TCTTGCG	370	0.0	16.8704	2
CAATACT	95	7.702529E-7	16.8397	4
ACGAACG	105	1.1888369E-7	16.76188	15
CGCAAGA	325	0.0	16.743954	2
AGCGAAA	335	0.0	16.715607	17
GACGGAC	335	0.0	16.71482	7
GTAGAAC	115	1.8435458E-8	16.693272	3
CTTGCGC	395	0.0	16.605225	3
>>END_MODULE