FastQCFastQC Report
Thu 2 Feb 2017
SRR4063008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062397
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36190.3406447872123133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25360.2387054933325301No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15650.1473083978964549No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15160.14269618607733267No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15150.14260205930551384No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12730.11982338052535917No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12700.1195410002099027No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12560.11822322540443922No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12210.11492878839078047No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11820.11125784428984646No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11370.10702213955799951No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11090.1043865899470725No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11010.10363357577252195No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT10990.1034453222288843No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10780.10146866002068906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGC2900.019.86139728
AAACGCT2900.019.30969229
ACGAACG1208.185452E-1118.66603715
TCGCGTA1550.018.582649
CGCTTCG3050.018.36003332
CGAACGA1251.4733814E-1017.91939416
ACGCTTC3150.017.77717631
TCCAATA3000.017.5994052
AACGCTT3200.017.49940730
CGCGTAA1650.017.4564210
GTAAACG3300.017.45395527
AATAGCG2300.017.3931715
TAGGACG652.100002E-417.2309974
CAAGACG3350.016.716644
AACCGCG1252.8485374E-916.6417887
CCGATAA1354.4201443E-1016.5943749
GCGTAAC1750.016.45813611
CGCGGTC1304.791218E-916.00171910
TAGCGTA2300.016.0017197
GTATTAC703.6943713E-416.0009651
Produced by FastQC (version 0.11.5)