##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063008_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1062397
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.183011623715053	32.0	32.0	32.0	32.0	32.0
2	31.2995584513134	32.0	32.0	32.0	32.0	32.0
3	31.391299109466612	32.0	32.0	32.0	32.0	32.0
4	31.485147266040848	32.0	32.0	32.0	32.0	32.0
5	31.403922450835235	32.0	32.0	32.0	32.0	32.0
6	34.93842226587613	36.0	36.0	36.0	36.0	36.0
7	34.96939656267855	36.0	36.0	36.0	36.0	36.0
8	34.90222863957636	36.0	36.0	36.0	36.0	36.0
9	35.017002118793634	36.0	36.0	36.0	36.0	36.0
10	34.87015776588225	36.0	36.0	36.0	32.0	36.0
11	35.03800368412185	36.0	36.0	36.0	36.0	36.0
12	34.93404066464796	36.0	36.0	36.0	32.0	36.0
13	34.988744320625905	36.0	36.0	36.0	36.0	36.0
14	34.92996309289277	36.0	36.0	36.0	32.0	36.0
15	34.91111232430062	36.0	36.0	36.0	32.0	36.0
16	34.92559466941266	36.0	36.0	36.0	32.0	36.0
17	34.886498173469995	36.0	36.0	36.0	32.0	36.0
18	34.879004741165495	36.0	36.0	36.0	32.0	36.0
19	34.875266025788854	36.0	36.0	36.0	32.0	36.0
20	34.86641528543473	36.0	36.0	36.0	32.0	36.0
21	34.85584390769176	36.0	36.0	36.0	32.0	36.0
22	34.83963716011999	36.0	36.0	36.0	32.0	36.0
23	34.778847267076245	36.0	36.0	36.0	32.0	36.0
24	34.756381089178525	36.0	36.0	36.0	32.0	36.0
25	34.73929802136113	36.0	36.0	36.0	32.0	36.0
26	34.67260732099206	36.0	36.0	36.0	32.0	36.0
27	34.66315040422742	36.0	36.0	36.0	32.0	36.0
28	34.635115686508904	36.0	36.0	36.0	32.0	36.0
29	34.59685409503227	36.0	36.0	36.0	32.0	36.0
30	34.57786590135326	36.0	36.0	36.0	32.0	36.0
31	34.56491499881871	36.0	36.0	36.0	32.0	36.0
32	34.54770768366251	36.0	36.0	36.0	32.0	36.0
33	34.50492988967401	36.0	36.0	36.0	32.0	36.0
34	34.485612252293635	36.0	36.0	36.0	32.0	36.0
35	34.45948171916901	36.0	36.0	36.0	32.0	36.0
36	34.424264187493	36.0	36.0	36.0	32.0	36.0
37	34.42507085392749	36.0	36.0	36.0	32.0	36.0
38	33.99511670307804	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	5.0
21	18.0
22	78.0
23	225.0
24	653.0
25	1627.0
26	3431.0
27	6874.0
28	12150.0
29	19180.0
30	28681.0
31	42225.0
32	60549.0
33	97088.0
34	237923.0
35	551687.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.341312957208444	18.316484497156612	11.898739420251928	26.443463125383015
2	15.727924683522263	20.456947826471648	36.8324647001074	26.982662789898693
3	18.03873693167432	24.33864176950801	28.730126308715104	28.89249499010257
4	11.806071946942472	15.831038202399487	36.04265460836563	36.32023524229241
5	13.902335944096228	37.21885509842366	33.439571083126175	15.439237874353937
6	34.61568509700235	35.97299314662975	16.229902757632033	13.181418998735877
7	30.170784418557368	30.70655652527504	20.637862483904247	18.484796572263345
8	28.342507737373584	33.2435032417901	19.054929704737305	19.35905931609901
9	27.37088035182445	13.833320154977466	18.39707892329067	40.39872056990742
10	15.533142566689884	27.021310642143114	31.676707016321842	25.76883977484516
11	38.01208839722838	21.13643359335656	21.639944236361018	19.211533773054036
12	24.29040046310459	24.14318591484335	28.72895674396058	22.837456878091484
13	29.661040082003243	19.191319252595783	25.194724759200188	25.95291590620079
14	23.419023208838126	19.62166685335143	25.172604967822764	31.786704969987678
15	25.086667225152183	27.253747892736897	22.498934014309153	25.16065086780177
16	25.71675183570737	25.952445272341695	23.766445123621395	24.564357768329543
17	23.82988656782728	26.065209144980646	25.239529102585944	24.86537518460613
18	24.881564989358967	24.83600763179866	26.33949455806069	23.942932820781685
19	25.29977023654999	25.283956938884426	25.60963556937755	23.806637255188033
20	25.50355469753774	24.218536008667193	25.468068904562042	24.809840389233027
21	26.831589321129485	24.406883679076653	24.58290074237785	24.178626257416013
22	25.50440183848411	24.45931229097974	25.39079082489879	24.645495045637364
23	24.026540009563288	24.142692555318355	26.03360705424343	25.797160380874928
24	24.524730397393817	25.194159998569273	25.57170248033456	24.709407123702345
25	24.814829108139424	24.48303223747808	25.700279650639075	25.00185900374342
26	24.465430531147963	25.4778580888312	25.87874401000756	24.17796737001328
27	25.194890031381956	24.82497077355482	25.170793509752503	24.809345685310724
28	24.20987634565986	24.77426046948551	26.122720602561945	24.893142582292683
29	24.200934302337075	25.1438021756462	26.040924437851388	24.614339084165334
30	24.271811761516645	25.159050712680852	26.32735220449606	24.24178532130644
31	24.731715168623406	25.097585930683163	25.12902427247065	25.041674628222783
32	24.381092943598297	25.02444942897994	25.203572675751158	25.39088495167061
33	24.037341972915964	24.60134958965434	25.79600657757882	25.565301859850887
34	24.850503154658757	24.96364353438498	25.860859923361986	24.32499338759428
35	25.553253633058077	24.592972306962462	25.789229450007863	24.064544609971602
36	24.243291349655543	25.400768262711587	25.506849134551395	24.849091253081475
37	25.37074182250138	25.273320613668904	25.146908359116228	24.20902920471349
38	24.389376099518355	24.940582475289368	25.781511054718713	24.88853037047356
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	52.0
2	71.0
3	71.0
4	301.0
5	531.0
6	531.0
7	750.0
8	969.0
9	1008.5
10	1048.0
11	1048.0
12	1415.5
13	1783.0
14	2288.5
15	2794.0
16	2794.0
17	4161.5
18	5529.0
19	5529.0
20	6547.0
21	7565.0
22	8054.0
23	8543.0
24	8543.0
25	9935.5
26	11328.0
27	11328.0
28	14389.5
29	17451.0
30	21343.5
31	25236.0
32	25236.0
33	31481.5
34	37727.0
35	37727.0
36	42121.0
37	46515.0
38	52995.5
39	59476.0
40	59476.0
41	63460.0
42	67444.0
43	75808.5
44	84173.0
45	84173.0
46	87148.0
47	90123.0
48	90123.0
49	94624.0
50	99125.0
51	98776.0
52	98427.0
53	98427.0
54	93284.5
55	88142.0
56	88142.0
57	85604.0
58	83066.0
59	74026.0
60	64986.0
61	64986.0
62	61302.5
63	57619.0
64	47914.0
65	38209.0
66	38209.0
67	32024.5
68	25840.0
69	25840.0
70	20679.0
71	15518.0
72	12233.5
73	8949.0
74	8949.0
75	6795.5
76	4642.0
77	4642.0
78	4447.0
79	4252.0
80	3296.0
81	2340.0
82	2340.0
83	2149.0
84	1958.0
85	1958.0
86	1264.0
87	570.0
88	438.5
89	307.0
90	307.0
91	186.0
92	65.0
93	41.5
94	18.0
95	18.0
96	17.5
97	17.0
98	17.0
99	12.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01016569135643267
2	0.0
3	0.0
4	2.823803154564631E-4
5	0.0
6	0.0
7	4.706338590941051E-4
8	0.0019766622081952416
9	0.00461221181912223
10	0.0016001551209199572
11	0.0052710992218539775
12	1.8825354363764205E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	9.412677181882103E-5
24	0.0
25	0.0
26	0.0
27	2.823803154564631E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1062397.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.94589408580508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.74434975827141	42.167869135019615
2	14.495478011257099	15.929339990592977
3	4.357311183272404	7.182490763249386
4	1.7832684011594317	3.919331067866764
5	0.8163749760601696	2.2428226484451868
6	0.44411175557387855	1.464127049041397
7	0.2662788286843641	1.0241649822728314
8	0.16378694064375637	0.7199535914599904
9	0.13100940890697907	0.647858619544212
>10	0.626478793272091	6.461802989343417
>50	0.0759111018495616	2.9393018366869272
>100	0.0834496394911799	9.567437676881093
>500	0.009443888530615098	3.5171238491598706
>1k	0.0027473130270880284	2.2163758004362695
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3619	0.3406447872123133	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2536	0.2387054933325301	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1565	0.1473083978964549	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1516	0.14269618607733267	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1515	0.14260205930551384	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1273	0.11982338052535917	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1270	0.1195410002099027	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1256	0.11822322540443922	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1221	0.11492878839078047	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1182	0.11125784428984646	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1137	0.10702213955799951	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1109	0.1043865899470725	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1101	0.10363357577252195	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1099	0.1034453222288843	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1078	0.10146866002068906	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	3.765070872752841E-4
13	0.0	0.0	0.0	0.0	5.647606309129262E-4
14	0.0	0.0	0.0	0.0	5.647606309129262E-4
15	0.0	0.0	0.0	9.412677181882103E-5	9.412677181882103E-4
16	0.0	0.0	0.0	1.8825354363764205E-4	9.412677181882103E-4
17	0.0	0.0	0.0	2.823803154564631E-4	9.412677181882103E-4
18	0.0	0.0	0.0	2.823803154564631E-4	9.412677181882103E-4
19	0.0	0.0	0.0	2.823803154564631E-4	9.412677181882103E-4
20	0.0	0.0	0.0	4.7063385909410513E-4	0.0012236480336446733
21	0.0	0.0	0.0	5.647606309129262E-4	0.0013177748054634944
22	0.0	0.0	0.0	9.412677181882103E-4	0.0013177748054634944
23	0.0	0.0	0.0	0.0014119015772823154	0.0013177748054634944
24	0.0	0.0	0.0	0.0023531692954705257	0.0013177748054634944
25	0.0	0.0	0.0	0.002917929926383452	0.0013177748054634944
26	0.0	0.0	0.0	0.003388563785477557	0.0014119015772823154
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACGC	290	0.0	19.861397	28
AAACGCT	290	0.0	19.309692	29
ACGAACG	120	8.185452E-11	18.666037	15
TCGCGTA	155	0.0	18.58264	9
CGCTTCG	305	0.0	18.360033	32
CGAACGA	125	1.4733814E-10	17.919394	16
ACGCTTC	315	0.0	17.777176	31
TCCAATA	300	0.0	17.599405	2
AACGCTT	320	0.0	17.499407	30
CGCGTAA	165	0.0	17.45642	10
GTAAACG	330	0.0	17.453955	27
AATAGCG	230	0.0	17.393171	5
TAGGACG	65	2.100002E-4	17.230997	4
CAAGACG	335	0.0	16.71664	4
AACCGCG	125	2.8485374E-9	16.641788	7
CCGATAA	135	4.4201443E-10	16.594374	9
GCGTAAC	175	0.0	16.458136	11
CGCGGTC	130	4.791218E-9	16.001719	10
TAGCGTA	230	0.0	16.001719	7
GTATTAC	70	3.6943713E-4	16.000965	1
>>END_MODULE