FastQCFastQC Report
Thu 2 Feb 2017
SRR4063007_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063007_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066223
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT44230.21406208332788862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26740.129414879226492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27750.018.986991
TAATACC1800.016.8845354
CGTCGTA3650.016.21970610
CGAGCCG3850.015.79233915
ACCGTCG3950.015.3906718
GGTATCA11250.014.80485251
ATACCGT4450.014.379016
TACCGTC4150.014.2634537
CGACGGT4650.014.1059377
GTTCGCG800.001018364114.00011716
TACGACG4700.013.9538485
GTCGTAG4600.013.91349711
GTTATTC3150.013.7117443
AACGCAG37200.013.6745546
GACGGTA4950.013.5742278
CGTTATT2950.013.5637032
GTGCTAG2850.013.4862011
CCGTCGT4750.013.4741229
CGTTTAT3450.013.44938731
TGTAGGA6550.013.4394552
Produced by FastQC (version 0.11.5)