##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063007_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2066223
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238876926643446	32.0	32.0	32.0	32.0	32.0
2	30.797655916132964	32.0	32.0	32.0	32.0	32.0
3	30.84816643702059	32.0	32.0	32.0	32.0	32.0
4	30.926428076737118	32.0	32.0	32.0	32.0	32.0
5	30.805829767648508	32.0	32.0	32.0	32.0	32.0
6	34.48795265564269	36.0	36.0	36.0	32.0	36.0
7	34.41636744920563	36.0	36.0	36.0	32.0	36.0
8	34.39703362124998	36.0	36.0	36.0	32.0	36.0
9	34.56858093245501	36.0	36.0	36.0	32.0	36.0
10	34.2313878995636	36.0	36.0	36.0	32.0	36.0
11	34.556206179100705	36.0	36.0	36.0	32.0	36.0
12	34.35115667573152	36.0	36.0	36.0	32.0	36.0
13	34.44401693331262	36.0	36.0	36.0	32.0	36.0
14	34.346413722042584	36.0	36.0	36.0	32.0	36.0
15	34.27232539759745	36.0	36.0	36.0	32.0	36.0
16	34.292729293982305	36.0	36.0	36.0	32.0	36.0
17	34.22159805596976	36.0	36.0	36.0	32.0	36.0
18	34.245361705875894	36.0	36.0	36.0	32.0	36.0
19	34.23152002470208	36.0	36.0	36.0	32.0	36.0
20	34.22596641311223	36.0	36.0	36.0	32.0	36.0
21	34.216811060568006	36.0	36.0	36.0	32.0	36.0
22	34.18521330950241	36.0	36.0	36.0	32.0	36.0
23	34.13043558221934	36.0	36.0	36.0	32.0	36.0
24	34.11279808616979	36.0	36.0	36.0	32.0	36.0
25	34.093527658921616	36.0	36.0	36.0	32.0	36.0
26	34.04690297223485	36.0	36.0	36.0	32.0	36.0
27	34.05159220471362	36.0	36.0	36.0	32.0	36.0
28	34.031366895054404	36.0	36.0	36.0	32.0	36.0
29	34.00104683763563	36.0	36.0	36.0	32.0	36.0
30	33.97346704590937	36.0	36.0	36.0	32.0	36.0
31	33.993585397123155	36.0	36.0	36.0	32.0	36.0
32	33.95318559516567	36.0	36.0	36.0	32.0	36.0
33	33.919930714158156	36.0	36.0	36.0	32.0	36.0
34	33.915469917816225	36.0	36.0	36.0	32.0	36.0
35	33.89517394782654	36.0	36.0	36.0	32.0	36.0
36	33.840597070112956	36.0	36.0	36.0	32.0	36.0
37	33.844937840688054	36.0	36.0	36.0	32.0	36.0
38	33.26450533170912	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	6.0
9	8.0
10	13.0
11	23.0
12	10.0
13	9.0
14	198.0
15	453.0
16	655.0
17	744.0
18	932.0
19	1110.0
20	1566.0
21	2312.0
22	3531.0
23	5751.0
24	9083.0
25	13810.0
26	20550.0
27	29758.0
28	41626.0
29	56137.0
30	75649.0
31	101658.0
32	138502.0
33	208661.0
34	458267.0
35	895197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.21315747520605	17.048285392950266	11.40088064741686	26.337676484426826
2	16.70094256708497	19.52147711617569	37.66220119558085	26.11537912115849
3	18.755170052057494	23.767887956592133	28.5624048577313	28.914537133619074
4	12.799062224883082	15.289325596624087	35.979376304045125	35.93223587444771
5	14.667135800772233	36.349262275326225	33.88916367972789	15.094438244173656
6	34.28183003043726	35.39584661243229	16.823015201210715	13.499308155919731
7	30.080054282620992	30.21919705665845	21.26643639142532	18.43431226929523
8	27.947513715894175	33.08753779856511	19.547569082806444	19.417379402734277
9	27.853304927166445	13.874576641961303	18.359243797121117	39.91287463375113
10	15.717361045236403	26.73805979250919	32.074637065315656	25.469942096938748
11	37.461329678286155	20.950747852079	22.477426986173548	19.1104954834613
12	24.773494171830805	23.45317814780183	29.053921938735012	22.71940574163235
13	29.848140369355846	19.452728347308536	24.929946498438227	25.76918478489739
14	23.628849851961814	19.31437289359595	25.193403164827462	31.863374089614773
15	25.186628126786587	27.122884352766736	22.221974799333314	25.468512721113363
16	25.675910495253852	25.547207127233374	23.81002627794603	24.966856099566744
17	24.215021096066007	25.6425635157141	25.232163222944465	24.91025216527543
18	24.988529157236698	24.76888913841871	25.99772131950508	24.244860384839516
19	25.764409838771275	24.615223774981594	25.270622355185278	24.349744031061856
20	26.00039591366759	24.241048503296295	24.88486801506408	24.873687567972034
21	26.824568714543553	23.97275821829726	24.483142320449584	24.71953074670961
22	26.063947185007358	24.20312863006273	24.760609463331527	24.97231472159839
23	24.727363723003187	24.0217595960564	25.412106594721955	25.838770086218453
24	25.055507930845373	24.65370166623022	25.18697471974969	25.10381568317472
25	25.10193086562939	24.3461200923005	25.289503988893735	25.262445053176375
26	24.869555620205603	24.977018844305636	25.460119284178752	24.69330625131001
27	25.390104494133485	24.640170683270338	24.79795977733345	25.171765045262724
28	24.765851007139542	24.66028975378237	25.285719609757322	25.288139629320767
29	24.866044072105318	24.818076475160915	25.228826250811963	25.0870532019218
30	24.746134974254137	24.95120167538091	25.425899387123053	24.876763963241903
31	25.340617918414328	24.612143276843597	24.633294258065202	25.413944546676877
32	25.086383350894764	24.64753317996729	24.586257750785183	25.679825718352767
33	24.696933175737616	24.48657967925206	25.141598976983087	25.674888168027238
34	25.411245709661134	24.53906840292181	25.189655848389553	24.860030039027503
35	25.693972158340905	24.481617428168008	25.223571165455006	24.60083924803608
36	24.805896111339152	24.97879862762258	24.950288106320524	25.26501715471774
37	25.473045608794077	24.770392642496926	24.77319996302081	24.98336178568818
38	24.92369659850907	24.795338450235082	25.0914527606198	25.189512190636048
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	75.0
1	122.5
2	170.0
3	170.0
4	454.0
5	738.0
6	738.0
7	932.5
8	1127.0
9	1064.5
10	1002.0
11	1002.0
12	1458.0
13	1914.0
14	2649.5
15	3385.0
16	3385.0
17	5232.0
18	7079.0
19	7079.0
20	9209.5
21	11340.0
22	12483.0
23	13626.0
24	13626.0
25	16546.0
26	19466.0
27	19466.0
28	24979.5
29	30493.0
30	37986.5
31	45480.0
32	45480.0
33	56700.5
34	67921.0
35	67921.0
36	77514.0
37	87107.0
38	99345.5
39	111584.0
40	111584.0
41	120636.5
42	129689.0
43	145317.0
44	160945.0
45	160945.0
46	166591.0
47	172237.0
48	172237.0
49	183631.5
50	195026.0
51	197639.0
52	200252.0
53	200252.0
54	193196.5
55	186141.0
56	186141.0
57	178885.5
58	171630.0
59	154264.5
60	136899.0
61	136899.0
62	125832.5
63	114766.0
64	95168.0
65	75570.0
66	75570.0
67	62772.5
68	49975.0
69	49975.0
70	39559.5
71	29144.0
72	22535.0
73	15926.0
74	15926.0
75	12001.0
76	8076.0
77	8076.0
78	7265.0
79	6454.0
80	5042.0
81	3630.0
82	3630.0
83	3552.0
84	3474.0
85	3474.0
86	2319.0
87	1164.0
88	995.0
89	826.0
90	826.0
91	630.0
92	434.0
93	380.0
94	326.0
95	326.0
96	289.0
97	252.0
98	252.0
99	566.0
100	880.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12167128136701605
2	0.054108390043088275
3	0.011518601815970492
4	0.0027102592508165867
5	2.4198743310862382E-4
6	2.9038491973034855E-4
7	0.0
8	3.3878240635207334E-4
9	2.4198743310862382E-4
10	5.323723528389724E-4
11	0.0018875019782472655
12	0.0024682718177079627
13	0.014616040959760879
14	0.0097762922975884
15	0.023424383524914785
16	0.011228216896240146
17	0.02105290668045027
18	0.005565710961498348
19	0.0073564179665021635
20	0.005614108448120073
21	0.006388468234067668
22	0.006920840586906641
23	0.009292317431371154
24	0.014616040959760879
25	0.017519890157064365
26	0.019358994648689905
27	0.008421162672180108
28	0.00600128834109387
29	0.011857384222322567
30	0.003194234117033834
31	0.006049685827715595
32	0.0068724431002849164
33	0.008566355132045282
34	0.011808986735700841
35	0.01577758063868227
36	0.015438798232330199
37	0.009485907377858052
38	0.006243275774202494
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2066223.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.51168467451144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.61142698129748	43.638527463991664
2	12.466369588950247	13.840583553154499
3	3.817315948371726	6.357169176869845
4	1.602331158983028	3.557924081664412
5	0.8653613202363084	2.401883236923843
6	0.5325740496577877	1.773844962625842
7	0.37615363584573575	1.461664541556764
8	0.2792916393278605	1.2403159531676455
9	0.2049422597823397	1.0239021091367146
>10	1.1117228010185327	10.910892416673526
>50	0.06893637166938413	2.6429067888641757
>100	0.05375527522784841	6.307217235152327
>500	0.007188888480461315	2.701053024313666
>1k	0.002630081151388279	2.1421154559051203
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4423	0.21406208332788862	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2674	0.129414879226492	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.839748662172476E-5
2	0.0	0.0	0.0	0.0	4.839748662172476E-5
3	0.0	0.0	0.0	0.0	4.839748662172476E-5
4	0.0	0.0	0.0	0.0	4.839748662172476E-5
5	0.0	0.0	0.0	0.0	4.839748662172476E-5
6	0.0	0.0	0.0	0.0	4.839748662172476E-5
7	0.0	0.0	0.0	0.0	4.839748662172476E-5
8	0.0	0.0	0.0	0.0	4.839748662172476E-5
9	0.0	0.0	0.0	9.679497324344952E-5	4.839748662172476E-5
10	0.0	0.0	0.0	9.679497324344952E-5	4.839748662172476E-5
11	0.0	0.0	0.0	9.679497324344952E-5	4.839748662172476E-5
12	0.0	0.0	0.0	9.679497324344952E-5	9.679497324344952E-5
13	0.0	0.0	0.0	9.679497324344952E-5	1.4519245986517428E-4
14	0.0	0.0	0.0	9.679497324344952E-5	1.4519245986517428E-4
15	0.0	0.0	0.0	1.9358994648689903E-4	3.8717989297379807E-4
16	0.0	0.0	0.0	1.9358994648689903E-4	3.8717989297379807E-4
17	0.0	0.0	0.0	1.9358994648689903E-4	3.8717989297379807E-4
18	0.0	0.0	0.0	1.9358994648689903E-4	4.3557737959552286E-4
19	0.0	0.0	0.0	2.419874331086238E-4	4.3557737959552286E-4
20	0.0	0.0	0.0	2.419874331086238E-4	6.291673260824218E-4
21	0.0	0.0	0.0	2.419874331086238E-4	6.291673260824218E-4
22	0.0	0.0	0.0	3.3878240635207334E-4	6.291673260824218E-4
23	0.0	0.0	0.0	6.291673260824218E-4	6.775648127041467E-4
24	0.0	0.0	0.0	0.001209937165543119	6.775648127041467E-4
25	0.0	0.0	0.0	0.0018391044916255409	7.259622993258714E-4
26	0.0	0.0	0.0	0.002177886897977614	7.259622993258714E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2775	0.0	18.98699	1
TAATACC	180	0.0	16.884535	4
CGTCGTA	365	0.0	16.219706	10
CGAGCCG	385	0.0	15.792339	15
ACCGTCG	395	0.0	15.390671	8
GGTATCA	1125	0.0	14.8048525	1
ATACCGT	445	0.0	14.37901	6
TACCGTC	415	0.0	14.263453	7
CGACGGT	465	0.0	14.105937	7
GTTCGCG	80	0.0010183641	14.000117	16
TACGACG	470	0.0	13.953848	5
GTCGTAG	460	0.0	13.913497	11
GTTATTC	315	0.0	13.711744	3
AACGCAG	3720	0.0	13.674554	6
GACGGTA	495	0.0	13.574227	8
CGTTATT	295	0.0	13.563703	2
GTGCTAG	285	0.0	13.486201	1
CCGTCGT	475	0.0	13.474122	9
CGTTTAT	345	0.0	13.449387	31
TGTAGGA	655	0.0	13.439455	2
>>END_MODULE