FastQCFastQC Report
Thu 2 Feb 2017
SRR4063007_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063007_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066223
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT44340.2145944556807276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28890.13982033885016285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30100.017.9684091
TCTAGCG4250.017.6935828
CTAGCGG4750.016.1679329
AACGAAT908.589901E-615.999516531
ATACCGT4450.015.8216616
ACCGTCG4100.015.6111748
CCGTCGT4350.015.0814649
GTATAGT1704.7293724E-1115.060191
TACCGTC4550.014.7705727
TTATCCG650.00416391114.76878427
CGTCGTA4200.014.47680510
GTCCTAA3650.014.4670681
TTTTCGG3700.014.26983729
CTAGGAC3600.014.222483
CGGTCCA5700.014.03568310
TAGGACG4250.013.934
GTATTAC1155.6137087E-613.9143071
GATATAC2201.8189894E-1213.8194361
GTATAGG1751.1714292E-913.7155291
TAGCGGC5500.013.67231430
Produced by FastQC (version 0.11.5)