##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063007_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2066223
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23855121155848	32.0	32.0	32.0	32.0	32.0
2	31.297020215146187	32.0	32.0	32.0	32.0	32.0
3	31.380989370460014	32.0	32.0	32.0	32.0	32.0
4	31.47841931872794	32.0	32.0	32.0	32.0	32.0
5	31.40287181006116	32.0	32.0	32.0	32.0	32.0
6	34.93798491256752	36.0	36.0	36.0	36.0	36.0
7	34.966031740039675	36.0	36.0	36.0	36.0	36.0
8	34.89957569923479	36.0	36.0	36.0	36.0	36.0
9	35.01602731166965	36.0	36.0	36.0	36.0	36.0
10	34.85960179515958	36.0	36.0	36.0	32.0	36.0
11	35.04153036724497	36.0	36.0	36.0	36.0	36.0
12	34.94284353624948	36.0	36.0	36.0	36.0	36.0
13	34.996266617882	36.0	36.0	36.0	36.0	36.0
14	34.93431299525753	36.0	36.0	36.0	32.0	36.0
15	34.90859215099241	36.0	36.0	36.0	32.0	36.0
16	34.927377635424634	36.0	36.0	36.0	32.0	36.0
17	34.898405448008276	36.0	36.0	36.0	32.0	36.0
18	34.89541206346072	36.0	36.0	36.0	32.0	36.0
19	34.89095707481719	36.0	36.0	36.0	32.0	36.0
20	34.874218804069066	36.0	36.0	36.0	32.0	36.0
21	34.87067175227456	36.0	36.0	36.0	32.0	36.0
22	34.85487432866636	36.0	36.0	36.0	32.0	36.0
23	34.79815924999383	36.0	36.0	36.0	32.0	36.0
24	34.786759705994946	36.0	36.0	36.0	32.0	36.0
25	34.76233978617022	36.0	36.0	36.0	32.0	36.0
26	34.699008287101634	36.0	36.0	36.0	32.0	36.0
27	34.691679455702506	36.0	36.0	36.0	32.0	36.0
28	34.662152633089455	36.0	36.0	36.0	32.0	36.0
29	34.63242302500746	36.0	36.0	36.0	32.0	36.0
30	34.60910511595312	36.0	36.0	36.0	32.0	36.0
31	34.603113991084214	36.0	36.0	36.0	32.0	36.0
32	34.586762416254196	36.0	36.0	36.0	32.0	36.0
33	34.554539369661455	36.0	36.0	36.0	32.0	36.0
34	34.54430572111529	36.0	36.0	36.0	32.0	36.0
35	34.521242382840576	36.0	36.0	36.0	32.0	36.0
36	34.50037629045848	36.0	36.0	36.0	32.0	36.0
37	34.499673558952736	36.0	36.0	36.0	32.0	36.0
38	34.074559231989966	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	9.0
21	32.0
22	117.0
23	388.0
24	1216.0
25	3004.0
26	6319.0
27	12627.0
28	22756.0
29	36029.0
30	55143.0
31	79923.0
32	115605.0
33	187458.0
34	465133.0
35	1080462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.68219666479995	18.173660215786562	12.022034604648642	27.122108514764843
2	15.767078384085359	20.443582323882755	36.93052492397965	26.858814368052236
3	17.75152052803594	24.52276448379483	28.90583446220471	28.819880525964525
4	12.289337738152048	16.14637730118061	35.92793997934394	35.636344981323404
5	14.17767024921342	36.90824940797717	33.570803897550164	15.343276445259244
6	34.2945406130322	35.84621393355309	16.512560853848647	13.346684599566066
7	29.899622981207134	30.439790727951177	21.192424777733144	18.46816151310854
8	27.876672416429997	33.34412623924823	19.326706585898947	19.452494758422823
9	27.623502649856015	13.782639208189144	18.14771434794182	40.44614379401302
10	15.42738802885681	27.09231720809485	32.12222231901818	25.358072444030157
11	37.57350069235486	20.939898951521606	22.35819793169572	19.128402424427808
12	24.58719263891806	23.566729164105627	28.98629283066937	22.85978536630694
13	29.84517115094881	19.47375324082611	24.932908789347877	25.748166818877205
14	23.466065117881815	19.35484183209742	25.138150692423178	32.04094235759759
15	25.084465713526566	27.219617630817194	22.094710977469518	25.601205678186723
16	25.711818822865514	25.570691615272516	23.688705128831813	25.028784433030154
17	24.194435934553045	25.743881468747563	25.076044550854387	24.985638045845004
18	25.010901533861546	24.78599841353039	25.88738001658098	24.315720036027088
19	25.749737564628795	24.62275369115531	25.229367788472008	24.398140955743884
20	26.064611612589733	24.259578951545887	24.798097785185817	24.877711650678556
21	26.727876640494898	24.116103746888644	24.415829671656613	24.74018994095985
22	25.94272345504184	24.298368857929525	24.766566596699967	24.992341090328672
23	24.60879063759395	24.04365263928689	25.398783576393814	25.94877314672535
24	25.034664699792806	24.697431013012633	25.072511534331	25.19539275286356
25	25.106414076712998	24.340523109580243	25.299375042650325	25.253687771056438
26	24.879962017615735	25.001923801955357	25.441131418178404	24.676982762250503
27	25.384530874994375	24.67361881775359	24.685427827350342	25.256422479901698
28	24.750281068403556	24.629045364416136	25.28216944637631	25.338504120804
29	24.882503001854108	24.73750413193542	25.20047448895884	25.179518377251632
30	24.820118641598704	24.792677266684187	25.38090031908463	25.00630377263248
31	25.254824866435037	24.55088342352205	24.697769795418985	25.496521914623933
32	25.077060898073437	24.70106082450926	24.559352983680853	25.662525293736444
33	24.69960889991061	24.462412817977537	25.15202860485049	25.68594967726136
34	25.311304733322586	24.513472166363456	25.20933122901062	24.965891871303338
35	25.739767682384716	24.45800864669496	25.230335738204445	24.571887932715878
36	24.86803215335421	24.95195339515628	24.872533119610033	25.307481331879472
37	25.527980280928052	24.793451626470134	24.656002764464436	25.022565328137382
38	24.96250404723982	24.688525875474234	25.080303529677096	25.26866654760885
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	37.0
1	76.5
2	116.0
3	116.0
4	376.0
5	636.0
6	636.0
7	794.5
8	953.0
9	968.0
10	983.0
11	983.0
12	1399.0
13	1815.0
14	2586.5
15	3358.0
16	3358.0
17	5314.0
18	7270.0
19	7270.0
20	9218.5
21	11167.0
22	12450.0
23	13733.0
24	13733.0
25	16545.5
26	19358.0
27	19358.0
28	24876.5
29	30395.0
30	37701.5
31	45008.0
32	45008.0
33	57028.0
34	69048.0
35	69048.0
36	78358.0
37	87668.0
38	99702.0
39	111736.0
40	111736.0
41	121080.0
42	130424.0
43	146083.0
44	161742.0
45	161742.0
46	167391.5
47	173041.0
48	173041.0
49	184486.5
50	195932.0
51	198116.5
52	200301.0
53	200301.0
54	192762.5
55	185224.0
56	185224.0
57	178805.0
58	172386.0
59	155000.5
60	137615.0
61	137615.0
62	126531.0
63	115447.0
64	95440.0
65	75433.0
66	75433.0
67	62299.5
68	49166.0
69	49166.0
70	39136.5
71	29107.0
72	22335.0
73	15563.0
74	15563.0
75	11611.5
76	7660.0
77	7660.0
78	7023.5
79	6387.0
80	4861.0
81	3335.0
82	3335.0
83	3062.0
84	2789.0
85	2789.0
86	1764.0
87	739.0
88	614.5
89	490.0
90	490.0
91	291.0
92	92.0
93	62.5
94	33.0
95	33.0
96	23.5
97	14.0
98	14.0
99	18.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009679497324344952
2	0.0
3	0.0
4	4.839748662172476E-5
5	9.679497324344952E-5
6	9.679497324344952E-5
7	6.291673260824219E-4
8	0.0024682718177079627
9	0.0035330165233859075
10	0.00101634721905622
11	0.00416218384946833
12	2.4198743310862382E-4
13	2.9038491973034855E-4
14	4.839748662172476E-5
15	0.0
16	9.679497324344952E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	9.679497324344952E-5
22	9.679497324344952E-5
23	9.679497324344952E-5
24	0.0
25	9.679497324344952E-5
26	9.679497324344952E-5
27	1.9358994648689903E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2066223.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.49940718884354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.0150666046324	41.202604079571344
2	13.416732576356491	14.355744784926355
3	3.9880951772310596	6.400821833836427
4	1.7054141182424216	3.6495457734981556
5	0.9923567069955341	2.6545247772066993
6	0.5937461499077322	1.905904022443314
7	0.4138574415260619	1.5498789447635057
8	0.30330702945245525	1.2981397017532372
9	0.21577166342018533	1.0389290473017534
>10	1.2124801383078485	11.413208552734496
>50	0.0750439846478033	2.790138706643211
>100	0.057382927604329066	6.53509816763265
>500	0.007922516150902958	2.919984903025252
>1k	0.0028229655250343842	2.2854767046636493
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4434	0.2145944556807276	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2889	0.13982033885016285	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.839748662172476E-5
2	0.0	0.0	0.0	0.0	4.839748662172476E-5
3	0.0	0.0	0.0	0.0	4.839748662172476E-5
4	0.0	0.0	0.0	0.0	4.839748662172476E-5
5	0.0	0.0	0.0	0.0	4.839748662172476E-5
6	0.0	0.0	0.0	0.0	4.839748662172476E-5
7	0.0	0.0	0.0	0.0	4.839748662172476E-5
8	4.839748662172476E-5	0.0	0.0	0.0	4.839748662172476E-5
9	4.839748662172476E-5	0.0	0.0	0.0	4.839748662172476E-5
10	4.839748662172476E-5	0.0	0.0	0.0	4.839748662172476E-5
11	4.839748662172476E-5	0.0	0.0	0.0	4.839748662172476E-5
12	4.839748662172476E-5	0.0	0.0	0.0	2.419874331086238E-4
13	4.839748662172476E-5	0.0	0.0	0.0	2.419874331086238E-4
14	4.839748662172476E-5	0.0	0.0	0.0	2.419874331086238E-4
15	4.839748662172476E-5	0.0	0.0	4.839748662172476E-5	2.9038491973034855E-4
16	4.839748662172476E-5	0.0	0.0	9.679497324344952E-5	2.9038491973034855E-4
17	4.839748662172476E-5	0.0	0.0	9.679497324344952E-5	2.9038491973034855E-4
18	4.839748662172476E-5	0.0	0.0	9.679497324344952E-5	3.3878240635207334E-4
19	4.839748662172476E-5	0.0	0.0	1.4519245986517428E-4	3.3878240635207334E-4
20	4.839748662172476E-5	0.0	0.0	1.9358994648689903E-4	4.839748662172476E-4
21	4.839748662172476E-5	0.0	0.0	1.9358994648689903E-4	4.839748662172476E-4
22	4.839748662172476E-5	0.0	0.0	2.419874331086238E-4	4.839748662172476E-4
23	4.839748662172476E-5	0.0	0.0	4.839748662172476E-4	4.839748662172476E-4
24	4.839748662172476E-5	0.0	0.0	9.679497324344952E-4	4.839748662172476E-4
25	4.839748662172476E-5	0.0	0.0	0.0015003220852734677	5.323723528389724E-4
26	4.839748662172476E-5	0.0	0.0	0.001790707005003816	5.807698394606971E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3010	0.0	17.968409	1
TCTAGCG	425	0.0	17.69358	28
CTAGCGG	475	0.0	16.16793	29
AACGAAT	90	8.589901E-6	15.9995165	31
ATACCGT	445	0.0	15.821661	6
ACCGTCG	410	0.0	15.611174	8
CCGTCGT	435	0.0	15.081464	9
GTATAGT	170	4.7293724E-11	15.06019	1
TACCGTC	455	0.0	14.770572	7
TTATCCG	65	0.004163911	14.768784	27
CGTCGTA	420	0.0	14.476805	10
GTCCTAA	365	0.0	14.467068	1
TTTTCGG	370	0.0	14.269837	29
CTAGGAC	360	0.0	14.22248	3
CGGTCCA	570	0.0	14.035683	10
TAGGACG	425	0.0	13.93	4
GTATTAC	115	5.6137087E-6	13.914307	1
GATATAC	220	1.8189894E-12	13.819436	1
GTATAGG	175	1.1714292E-9	13.715529	1
TAGCGGC	550	0.0	13.672314	30
>>END_MODULE