FastQCFastQC Report
Thu 2 Feb 2017
SRR4063006_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063006_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835759
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT23810.2848907400339093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18290.21884299181941205No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15230.18222956617876684No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14690.17576837341865298No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14350.17170021501413685No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14320.17134125986079718No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13540.16200842587396605No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13470.16117086384950685No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13000.1555472331138522No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG12620.15100046783821652No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12550.1501629058137573No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12410.1484877817648389No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11870.14202658900472506No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC11460.1371208685757497No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT11150.13341166532457321No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC11110.13293305845345368No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT11100.1328134067356738No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA10970.1312579344045353No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG10580.1265915174111197No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT10510.1257539553866605No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG9910.11857485231986734No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA9770.11689972827094892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT9660.11558355937537018No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT9490.1135494801731121No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG9190.1099599286397155No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT9130.10924201833303619No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG8990.10756689428411778No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC8900.1064900288240988No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT8670.10373803931516141No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT8650.10349873587960165No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT8650.10349873587960165No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC8630.1032594324440419No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC8470.1013450049595637No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC8430.10086639808844415No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT8360.10002883606398495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG2650.022.33870528
CTAGCGG2850.020.77107429
AGCGTGC555.8326972E-520.3627998
TAGCGGC2850.020.20969430
AAGACGG3900.018.460785
AGCGGCG3050.018.359931
GACGGAC4100.017.950487
GCGCAAG4050.017.7908841
CGTTATT2700.017.7823662
GTATACC450.00884735417.778113
TTAAGGT450.00885040417.7770464
TATTCCC3150.017.7770465
GTTATTC2900.017.6555043
CGCAAGA3900.017.6455782
CAAGACG4200.017.1421534
GCAAGAC4300.017.11663
TAAGACC753.2331263E-517.0659644
CGGACCA4200.016.7622189
AGACGGA4300.016.7424966
CGGTCCA3450.016.69596510
Produced by FastQC (version 0.11.5)