##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063006_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	835759
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.198043933717734	32.0	32.0	32.0	32.0	32.0
2	30.78648270613897	32.0	32.0	32.0	32.0	32.0
3	30.844173978383722	32.0	32.0	32.0	32.0	32.0
4	30.91628687217248	32.0	32.0	32.0	32.0	32.0
5	30.81892387638063	32.0	32.0	32.0	32.0	32.0
6	34.476612276984156	36.0	36.0	36.0	32.0	36.0
7	34.38509666064021	36.0	36.0	36.0	32.0	36.0
8	34.36967475073556	36.0	36.0	36.0	32.0	36.0
9	34.525664695205194	36.0	36.0	36.0	32.0	36.0
10	34.21446613198302	36.0	36.0	36.0	32.0	36.0
11	34.51121914331763	36.0	36.0	36.0	32.0	36.0
12	34.30169702031327	36.0	36.0	36.0	32.0	36.0
13	34.419703526973684	36.0	36.0	36.0	32.0	36.0
14	34.300069756951466	36.0	36.0	36.0	32.0	36.0
15	34.246072133234584	36.0	36.0	36.0	32.0	36.0
16	34.25386265657923	36.0	36.0	36.0	32.0	36.0
17	34.16221183379419	36.0	36.0	36.0	32.0	36.0
18	34.20151861960206	36.0	36.0	36.0	32.0	36.0
19	34.17774741283073	36.0	36.0	36.0	32.0	36.0
20	34.17178516773376	36.0	36.0	36.0	32.0	36.0
21	34.14535410327618	36.0	36.0	36.0	32.0	36.0
22	34.12103130208589	36.0	36.0	36.0	32.0	36.0
23	34.063043293581046	36.0	36.0	36.0	32.0	36.0
24	34.04228372054624	36.0	36.0	36.0	32.0	36.0
25	34.02614390033491	36.0	36.0	36.0	32.0	36.0
26	33.964662061670886	36.0	36.0	36.0	32.0	36.0
27	33.986364490241805	36.0	36.0	36.0	32.0	36.0
28	33.96315325350968	36.0	36.0	36.0	32.0	36.0
29	33.921037045368344	36.0	36.0	36.0	32.0	36.0
30	33.89793229866505	36.0	36.0	36.0	32.0	36.0
31	33.90897615221613	36.0	36.0	36.0	32.0	36.0
32	33.85799734133883	36.0	36.0	36.0	32.0	36.0
33	33.81452906878658	36.0	36.0	36.0	32.0	36.0
34	33.828953083364944	36.0	36.0	36.0	32.0	36.0
35	33.783844385761924	36.0	36.0	36.0	32.0	36.0
36	33.73228646057057	36.0	36.0	36.0	27.0	36.0
37	33.738014188300696	36.0	36.0	36.0	27.0	36.0
38	33.12069029469021	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	10.0
11	3.0
12	6.0
13	4.0
14	125.0
15	264.0
16	350.0
17	418.0
18	535.0
19	617.0
20	829.0
21	1136.0
22	1732.0
23	2692.0
24	3989.0
25	6009.0
26	8814.0
27	12331.0
28	17381.0
29	22978.0
30	30829.0
31	41574.0
32	56235.0
33	84194.0
34	184583.0
35	358115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.610949713977654	17.42549941597532	11.254005810296805	25.709545059750216
2	16.93108707676173	19.859185955469144	37.295848368103854	25.913878599665264
3	18.67054222749813	24.220260774665416	28.641894349882254	28.467302647954202
4	12.286953233811797	15.266855882029168	36.25504348283899	36.19114740132004
5	14.65788418854808	36.71721975937924	33.31813749244695	15.306758559625727
6	34.03884143357459	35.93819734683646	16.855856427167225	13.167104792421721
7	30.144814474028998	30.50951290982209	21.095196103182857	18.250476512966056
8	28.37053309690126	32.575099490522376	19.57193350228176	19.48243391029461
9	27.7409250046066	13.808781967985947	18.811067318530007	39.639225708877454
10	16.305735532542432	26.038133184964497	31.05790572596036	26.598225556532718
11	38.063091090094574	20.91956388559176	22.04718191216449	18.970163112149173
12	25.024050542034605	23.91389666642736	28.367913465910448	22.69413932562759
13	29.683010567909978	18.731443930003604	25.33809294160305	26.247452560483364
14	23.721504052344834	20.246646778885445	24.608436870654035	31.42341229811569
15	25.231103600476807	27.501567818314992	22.197794043726574	25.06953453748163
16	26.06638984288056	25.371976976557733	23.82148455730133	24.740148623260378
17	24.058693576066844	25.911746613786118	25.090893424318512	24.938666385828526
18	25.418774148541424	23.966840290865406	26.607938371187238	24.006447189405932
19	25.76784314664018	24.697942406528774	25.194421342970134	24.339793103860906
20	26.07038112639956	23.796139077451787	24.68676222647411	25.446717569674536
21	27.906325572601748	23.821142200039965	23.871638608453	24.400893618905283
22	26.255606132402846	24.055273952604548	24.77443842946923	24.914681485523374
23	24.12232941396088	23.780092332214082	25.774760134884488	26.32281811894055
24	25.116228975569477	24.852836465681786	25.14327427644845	24.887660282300285
25	25.01573685583604	24.179230222422206	25.267765893027676	25.537267028714076
26	24.48826945078722	25.64696336507116	25.740788610764053	24.123978573377567
27	25.673911456644344	24.512463487861996	24.648400526038046	25.165224529455614
28	24.264456006164835	24.58705672316168	25.869681754871323	25.278805515802173
29	24.35156897870095	24.707929316680847	25.82152545678862	25.11897624782959
30	24.06154575128988	25.11642432538505	26.210310432959687	24.611719490365378
31	25.39741609240515	24.78787523438126	24.359736798965194	25.454971874248393
32	24.632613817621372	24.92554155134432	24.29732643612967	26.144518194904638
33	24.043801507516644	24.38184996870808	25.356985158176766	26.217363365598512
34	25.440616144596767	24.506012100788848	25.491235544152563	24.562136210461823
35	26.20668031722591	24.2475338641175	25.532569269008963	24.013216549647627
36	24.346123652516898	25.394791008482205	24.96110728167423	25.29797805732667
37	25.62335836299997	25.273102581745295	24.667843360157956	24.43569569509678
38	24.41788530135706	24.876062428877244	25.292119329474705	25.41393294029099
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	543.0
1	377.0
2	211.0
3	211.0
4	444.0
5	677.0
6	677.0
7	775.5
8	874.0
9	840.5
10	807.0
11	807.0
12	1081.5
13	1356.0
14	1693.5
15	2031.0
16	2031.0
17	3075.5
18	4120.0
19	4120.0
20	4705.0
21	5290.0
22	5637.0
23	5984.0
24	5984.0
25	7041.5
26	8099.0
27	8099.0
28	10411.0
29	12723.0
30	15969.5
31	19216.0
32	19216.0
33	25056.0
34	30896.0
35	30896.0
36	33745.5
37	36595.0
38	41613.5
39	46632.0
40	46632.0
41	48495.0
42	50358.0
43	57650.5
44	64943.0
45	64943.0
46	64647.0
47	64351.0
48	64351.0
49	69778.5
50	75206.0
51	75855.0
52	76504.0
53	76504.0
54	72626.0
55	68748.0
56	68748.0
57	67453.0
58	66158.0
59	58622.5
60	51087.0
61	51087.0
62	50311.0
63	49535.0
64	41241.5
65	32948.0
66	32948.0
67	27960.5
68	22973.0
69	22973.0
70	18483.5
71	13994.0
72	10897.0
73	7800.0
74	7800.0
75	5809.5
76	3819.0
77	3819.0
78	3922.5
79	4026.0
80	3150.0
81	2274.0
82	2274.0
83	2405.5
84	2537.0
85	2537.0
86	1664.5
87	792.0
88	704.5
89	617.0
90	617.0
91	439.0
92	261.0
93	250.5
94	240.0
95	240.0
96	196.5
97	153.0
98	153.0
99	267.0
100	381.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12371987618440244
2	0.056595262509886224
3	0.011366913189089198
4	0.0032305963800569304
5	4.786068711195452E-4
6	2.393034355597726E-4
7	0.0
8	1.196517177798863E-4
9	1.196517177798863E-4
10	4.786068711195452E-4
11	0.0017947757666982945
12	0.00227338263781784
13	0.01387959926246681
14	0.008375620244592041
15	0.02428929870931692
16	0.01064900288240988
17	0.01974253343368124
18	0.00550397901787477
19	0.008854227115711587
20	0.006221889324554088
21	0.006700496195673634
22	0.008016665091252382
23	0.009691789140170791
24	0.014836813004705901
25	0.01687089220696397
26	0.019503229998121468
27	0.009332833986831133
28	0.006102237606774202
29	0.010529351164629995
30	0.002751989508937385
31	0.005743282453434542
32	0.006939799631233405
33	0.009572137422390905
34	0.01328134067356738
35	0.016751240489184083
36	0.015195768158045561
37	0.010050744293510449
38	0.006700496195673634
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	835759.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.81292684088213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.34343735050574	42.43172078937256
2	12.753600009431814	13.47109888511992
3	3.461790563064281	5.4848187523671
4	1.3061850510919701	2.759338221758964
5	0.5923069401171732	1.564073154787751
6	0.32679659649119325	1.0355450845403198
7	0.19985537398420403	0.7388463071489433
8	0.12083094865948539	0.5105148841334229
9	0.08828221526571686	0.419619795856138
>10	0.5727039034762236	6.093721344380654
>50	0.09755039537167824	3.655457562971686
>100	0.11733260684171337	13.31793480157215
>500	0.014780270240282934	5.310529354034104
>1k	0.004547775458548595	3.206781061956371
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2381	0.2848907400339093	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1829	0.21884299181941205	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1523	0.18222956617876684	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1469	0.17576837341865298	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1435	0.17170021501413685	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1432	0.17134125986079718	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1354	0.16200842587396605	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1347	0.16117086384950685	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1300	0.1555472331138522	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1262	0.15100046783821652	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1255	0.1501629058137573	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1241	0.1484877817648389	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1187	0.14202658900472506	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1146	0.1371208685757497	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1115	0.13341166532457321	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1111	0.13293305845345368	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1110	0.1328134067356738	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1097	0.1312579344045353	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1058	0.1265915174111197	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1051	0.1257539553866605	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	991	0.11857485231986734	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	977	0.11689972827094892	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	966	0.11558355937537018	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	949	0.1135494801731121	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	919	0.1099599286397155	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	913	0.10924201833303619	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	899	0.10756689428411778	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	890	0.1064900288240988	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	867	0.10373803931516141	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	865	0.10349873587960165	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	865	0.10349873587960165	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	863	0.1032594324440419	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	847	0.1013450049595637	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	843	0.10086639808844415	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	836	0.10002883606398495	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.196517177798863E-4
2	0.0	0.0	0.0	0.0	1.196517177798863E-4
3	0.0	0.0	0.0	0.0	1.196517177798863E-4
4	0.0	0.0	0.0	0.0	1.196517177798863E-4
5	1.196517177798863E-4	0.0	0.0	0.0	1.196517177798863E-4
6	1.196517177798863E-4	0.0	0.0	0.0	1.196517177798863E-4
7	1.196517177798863E-4	0.0	0.0	0.0	1.196517177798863E-4
8	1.196517177798863E-4	0.0	0.0	0.0	1.196517177798863E-4
9	1.196517177798863E-4	0.0	0.0	1.196517177798863E-4	1.196517177798863E-4
10	1.196517177798863E-4	0.0	0.0	1.196517177798863E-4	1.196517177798863E-4
11	1.196517177798863E-4	0.0	0.0	1.196517177798863E-4	1.196517177798863E-4
12	1.196517177798863E-4	0.0	0.0	1.196517177798863E-4	1.196517177798863E-4
13	1.196517177798863E-4	0.0	0.0	1.196517177798863E-4	1.196517177798863E-4
14	1.196517177798863E-4	0.0	0.0	1.196517177798863E-4	1.196517177798863E-4
15	1.196517177798863E-4	0.0	0.0	1.196517177798863E-4	2.393034355597726E-4
16	1.196517177798863E-4	0.0	0.0	1.196517177798863E-4	2.393034355597726E-4
17	1.196517177798863E-4	0.0	0.0	1.196517177798863E-4	2.393034355597726E-4
18	1.196517177798863E-4	0.0	0.0	1.196517177798863E-4	2.393034355597726E-4
19	1.196517177798863E-4	0.0	0.0	1.196517177798863E-4	2.393034355597726E-4
20	1.196517177798863E-4	0.0	0.0	3.5895515333965894E-4	3.5895515333965894E-4
21	1.196517177798863E-4	0.0	0.0	4.786068711195452E-4	3.5895515333965894E-4
22	1.196517177798863E-4	0.0	0.0	8.375620244592041E-4	3.5895515333965894E-4
23	1.196517177798863E-4	0.0	0.0	0.0017947757666982947	3.5895515333965894E-4
24	1.196517177798863E-4	0.0	0.0	0.0033502480978368165	3.5895515333965894E-4
25	1.196517177798863E-4	0.0	0.0	0.0037092032511764753	3.5895515333965894E-4
26	1.196517177798863E-4	0.0	0.0	0.004307461840075907	3.5895515333965894E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	265	0.0	22.338705	28
CTAGCGG	285	0.0	20.771074	29
AGCGTGC	55	5.8326972E-5	20.362799	8
TAGCGGC	285	0.0	20.209694	30
AAGACGG	390	0.0	18.46078	5
AGCGGCG	305	0.0	18.3599	31
GACGGAC	410	0.0	17.95048	7
GCGCAAG	405	0.0	17.790884	1
CGTTATT	270	0.0	17.782366	2
GTATACC	45	0.008847354	17.77811	3
TTAAGGT	45	0.008850404	17.777046	4
TATTCCC	315	0.0	17.777046	5
GTTATTC	290	0.0	17.655504	3
CGCAAGA	390	0.0	17.645578	2
CAAGACG	420	0.0	17.142153	4
GCAAGAC	430	0.0	17.1166	3
TAAGACC	75	3.2331263E-5	17.065964	4
CGGACCA	420	0.0	16.762218	9
AGACGGA	430	0.0	16.742496	6
CGGTCCA	345	0.0	16.695965	10
>>END_MODULE