FastQCFastQC Report
Thu 2 Feb 2017
SRR4063006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835759
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29500.3529725674506646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21770.26048178960681245No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16610.19874150323239115No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15660.18737459004330195No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15030.1798365318231691No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14970.1791186215164898No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14070.16834996691630003No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13950.16691414630294138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13540.16200842587396605No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13050.15614549170275163No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12980.15530792967829243No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG12970.15518827796051254No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12790.1530345470404746No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC12640.15123977127377627No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA12270.1468126577159205No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT11410.13652260998685029No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC11110.13293305845345368No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11070.13245445158233415No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG10970.1312579344045353No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT10910.13054002409785595No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA10390.12431813477330186No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT10340.12371987618440244No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG10180.12180544869992427No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT9690.11594251452870982No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT9690.11594251452870982No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT9600.11486564906869084No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG9270.1109171423819546No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG9170.10972062520415574No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC9140.10936167005081608No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC9100.10888306317969655No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8970.10732759084855802No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT8920.1067293322596586No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG8890.10637037710631893No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT8810.10541316336407983No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC8620.103139780726262No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC8510.10182361183068325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG2550.018.19505728
TAGACTG802.789444E-618.0032985
CTAGCGG2500.017.91899529
GGACCGT450.00883897217.7810386
TAGCGGC2550.017.56764230
ATAGAGC652.0985198E-417.2318633
TCGCGTA2150.017.1183919
TCTAGAT2900.017.1035122
CGCGTAA2150.016.37313510
GCGTAAC2150.016.37313511
AGCGGCG2750.016.28999531
TATACTG1001.3108111E-616.0029345
GATATAC1102.0144034E-716.0019741
CTATACT805.61486E-516.0010194
TTAAGAC805.61486E-516.0010193
ACTATAC600.002440122216.0010173
TAACGAA1601.8189894E-1115.99910413
CGAACGA1703.6379788E-1215.99910316
ACGAACG1703.6379788E-1215.99910315
GTCGCGT2250.015.6463768
Produced by FastQC (version 0.11.5)