##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063006_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	835759
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.194047566343887	32.0	32.0	32.0	32.0	32.0
2	31.307318258014572	32.0	32.0	32.0	32.0	32.0
3	31.382232198516558	32.0	32.0	32.0	32.0	32.0
4	31.47810553042205	32.0	32.0	32.0	32.0	32.0
5	31.406875666310505	32.0	32.0	32.0	32.0	32.0
6	34.92598584041572	36.0	36.0	36.0	36.0	36.0
7	34.9609049977326	36.0	36.0	36.0	36.0	36.0
8	34.894019687493646	36.0	36.0	36.0	36.0	36.0
9	34.99269885218107	36.0	36.0	36.0	36.0	36.0
10	34.84692477137548	36.0	36.0	36.0	32.0	36.0
11	35.02302577656956	36.0	36.0	36.0	36.0	36.0
12	34.9149168600039	36.0	36.0	36.0	32.0	36.0
13	34.98150304094841	36.0	36.0	36.0	36.0	36.0
14	34.91758748634475	36.0	36.0	36.0	32.0	36.0
15	34.88922285012785	36.0	36.0	36.0	32.0	36.0
16	34.891447175561375	36.0	36.0	36.0	32.0	36.0
17	34.8562312819844	36.0	36.0	36.0	32.0	36.0
18	34.84955112658075	36.0	36.0	36.0	32.0	36.0
19	34.847807801052696	36.0	36.0	36.0	32.0	36.0
20	34.82845892177051	36.0	36.0	36.0	32.0	36.0
21	34.82139109480125	36.0	36.0	36.0	32.0	36.0
22	34.80747201047192	36.0	36.0	36.0	32.0	36.0
23	34.74627494289622	36.0	36.0	36.0	32.0	36.0
24	34.72148669652376	36.0	36.0	36.0	32.0	36.0
25	34.68655318100074	36.0	36.0	36.0	32.0	36.0
26	34.636431076422745	36.0	36.0	36.0	32.0	36.0
27	34.62169477086098	36.0	36.0	36.0	32.0	36.0
28	34.57578440674884	36.0	36.0	36.0	32.0	36.0
29	34.533097459913684	36.0	36.0	36.0	32.0	36.0
30	34.51104564832685	36.0	36.0	36.0	32.0	36.0
31	34.51579701803989	36.0	36.0	36.0	32.0	36.0
32	34.47643878199337	36.0	36.0	36.0	32.0	36.0
33	34.45351710241828	36.0	36.0	36.0	32.0	36.0
34	34.425085461239426	36.0	36.0	36.0	32.0	36.0
35	34.380978248514225	36.0	36.0	36.0	32.0	36.0
36	34.36883838522828	36.0	36.0	36.0	32.0	36.0
37	34.349970505851566	36.0	36.0	36.0	32.0	36.0
38	33.9086650577499	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	5.0
21	14.0
22	61.0
23	196.0
24	556.0
25	1417.0
26	3031.0
27	5809.0
28	9845.0
29	15782.0
30	23639.0
31	33654.0
32	48649.0
33	76986.0
34	189325.0
35	426788.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.031216083456684	18.58162885564162	11.930725591157497	26.456429469744197
2	15.867870842995222	20.893966913867413	36.67114164626602	26.567020596871345
3	17.7011554766386	25.013789860474134	28.932024662612072	28.353030000275197
4	11.796716274328215	16.099875801368338	36.360046807808	35.74336111649544
5	14.0188738619626	37.14922603286354	33.16709721343115	15.664802891742715
6	34.01168042864134	36.327664620218556	16.48457625841004	13.176078692730064
7	29.691871052965347	30.811937483996488	21.206479418584895	18.28971204445327
8	28.205388990129716	32.665622483520615	19.45243801608393	19.676550510265738
9	27.691678113827052	13.619089555882086	18.591305263460185	40.09792706683067
10	16.17649170332011	26.205734688114713	31.02616074548074	26.59161286308444
11	38.14268551845612	20.846894623985296	22.060231275846224	18.95018858171236
12	24.81388153029944	24.064621577059388	28.312860899925578	22.808635992715594
13	29.579974107337293	18.81872503763051	25.409747797807498	26.1915530572247
14	23.728371456364812	20.126256492601335	24.657108089772294	31.48826396126156
15	25.202121664259675	27.629496062860227	22.073588199468986	25.094794073411116
16	26.029305133782742	25.415850042715714	23.68592343716722	24.86892138633432
17	23.941949772601912	25.92780933259468	25.01534533280527	25.114895561998136
18	25.497900710611553	23.85699705297819	26.46731892806419	24.177783308346065
19	25.730264346540093	24.53302925843455	25.37226640694267	24.36443998808269
20	25.95461131737738	23.570191885459803	25.01139682611853	25.46379997104428
21	27.805895965099946	23.74634762694464	24.01029963219018	24.437456775765234
22	26.097236164971005	24.02821866112121	24.937571716248343	24.936973457659445
23	23.974643377628453	23.60144922333977	25.94112171226599	26.482785686765787
24	24.91065007974787	24.772930952583224	25.32955074369525	24.98686822397366
25	25.009273019223265	24.109969632357693	25.327188013755176	25.553569334663862
26	24.587590441742176	25.53200145017882	25.820481741746125	24.059926366332878
27	25.71973499537546	24.21774698208455	24.787169506999028	25.275348515540962
28	24.367431281027187	24.409668337403488	26.050571995036847	25.172328386532485
29	24.424744453843754	24.326989000417583	26.04458940914785	25.203677136590812
30	24.093069892157907	24.822586415461874	26.3322321386907	24.75211155368952
31	25.30155224173476	24.58615462112882	24.5410459235258	25.571247213610622
32	24.758812049885197	24.529798662054493	24.580650642110943	26.13073864594937
33	24.055858207928363	24.13099948669413	25.660866350227757	26.15227595514975
34	25.354318649275687	24.213439520244474	25.82263547266616	24.609606357813675
35	26.220956041155404	23.958102754502196	25.707530520161914	24.113410684180486
36	24.381430532007435	25.143492322547527	25.158209483834455	25.31686766161058
37	25.80385015297472	24.904787145576655	24.78346030374785	24.50790239770077
38	24.587619861251703	24.462583666563766	25.558235757240734	25.3915607149438
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	306.0
1	227.0
2	148.0
3	148.0
4	454.0
5	760.0
6	760.0
7	861.0
8	962.0
9	899.5
10	837.0
11	837.0
12	1090.5
13	1344.0
14	1714.0
15	2084.0
16	2084.0
17	3264.0
18	4444.0
19	4444.0
20	5039.0
21	5634.0
22	5923.0
23	6212.0
24	6212.0
25	7075.5
26	7939.0
27	7939.0
28	10398.0
29	12857.0
30	16066.0
31	19275.0
32	19275.0
33	25381.0
34	31487.0
35	31487.0
36	34008.0
37	36529.0
38	41776.0
39	47023.0
40	47023.0
41	48543.0
42	50063.0
43	57269.0
44	64475.0
45	64475.0
46	63858.0
47	63241.0
48	63241.0
49	69331.5
50	75422.0
51	75890.5
52	76359.0
53	76359.0
54	72386.0
55	68413.0
56	68413.0
57	67352.0
58	66291.0
59	59021.5
60	51752.0
61	51752.0
62	51111.0
63	50470.0
64	41758.0
65	33046.0
66	33046.0
67	28034.5
68	23023.0
69	23023.0
70	18686.0
71	14349.0
72	11058.0
73	7767.0
74	7767.0
75	5734.0
76	3701.0
77	3701.0
78	3882.0
79	4063.0
80	3150.5
81	2238.0
82	2238.0
83	2230.5
84	2223.0
85	2223.0
86	1392.0
87	561.0
88	459.5
89	358.0
90	358.0
91	212.5
92	67.0
93	44.5
94	22.0
95	22.0
96	15.0
97	8.0
98	8.0
99	7.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00813631680903227
2	1.196517177798863E-4
3	0.0
4	1.196517177798863E-4
5	0.0
6	2.393034355597726E-4
7	5.982585888994316E-4
8	0.0021537309200379533
9	0.004187810122296021
10	3.589551533396589E-4
11	0.005623630735654657
12	1.196517177798863E-4
13	1.196517177798863E-4
14	0.0
15	0.0
16	1.196517177798863E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.196517177798863E-4
22	0.0
23	1.196517177798863E-4
24	0.0
25	1.196517177798863E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.196517177798863E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	835759.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.631038141568766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.8477038116753	39.92141099064049
2	13.620559579020766	13.792461431098205
3	3.7970738311194068	5.767493699092779
4	1.4497792421925733	2.9361531243322734
5	0.6463255214697188	1.6362066064701322
6	0.33861341328400973	1.028660918593769
7	0.20567154546464583	0.7289354704139114
8	0.14338760294759922	0.580789055509441
9	0.09765102128930483	0.4449755325115743
>10	0.5978843328884358	6.08454881844258
>50	0.10794155003932976	3.8571705375817826
>100	0.12529726631855267	13.64270115136559
>500	0.016642900648778852	5.74829938027931
>1k	0.005468381641741623	3.8301932836682666
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2950	0.3529725674506646	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2177	0.26048178960681245	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1661	0.19874150323239115	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1566	0.18737459004330195	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1503	0.1798365318231691	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1497	0.1791186215164898	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1407	0.16834996691630003	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1395	0.16691414630294138	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1354	0.16200842587396605	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1305	0.15614549170275163	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1298	0.15530792967829243	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1297	0.15518827796051254	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1279	0.1530345470404746	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1264	0.15123977127377627	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1227	0.1468126577159205	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1141	0.13652260998685029	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1111	0.13293305845345368	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1107	0.13245445158233415	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1097	0.1312579344045353	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1091	0.13054002409785595	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1039	0.12431813477330186	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1034	0.12371987618440244	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1018	0.12180544869992427	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	969	0.11594251452870982	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	969	0.11594251452870982	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	960	0.11486564906869084	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	927	0.1109171423819546	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	917	0.10972062520415574	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	914	0.10936167005081608	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	910	0.10888306317969655	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	897	0.10732759084855802	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	892	0.1067293322596586	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	889	0.10637037710631893	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	881	0.10541316336407983	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	862	0.103139780726262	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	851	0.10182361183068325	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.196517177798863E-4	0.0
10	0.0	0.0	0.0	1.196517177798863E-4	0.0
11	0.0	0.0	0.0	1.196517177798863E-4	0.0
12	0.0	0.0	0.0	1.196517177798863E-4	1.196517177798863E-4
13	0.0	0.0	0.0	1.196517177798863E-4	1.196517177798863E-4
14	0.0	0.0	0.0	1.196517177798863E-4	1.196517177798863E-4
15	0.0	0.0	0.0	1.196517177798863E-4	1.196517177798863E-4
16	0.0	0.0	0.0	1.196517177798863E-4	3.5895515333965894E-4
17	0.0	0.0	0.0	1.196517177798863E-4	3.5895515333965894E-4
18	0.0	0.0	0.0	1.196517177798863E-4	4.786068711195452E-4
19	0.0	0.0	0.0	1.196517177798863E-4	4.786068711195452E-4
20	0.0	0.0	0.0	4.786068711195452E-4	5.982585888994316E-4
21	0.0	0.0	0.0	4.786068711195452E-4	7.179103066793179E-4
22	0.0	0.0	0.0	8.375620244592041E-4	7.179103066793179E-4
23	0.0	0.0	0.0	0.0016751240489184082	7.179103066793179E-4
24	0.0	0.0	0.0	0.0035895515333965893	7.179103066793179E-4
25	0.0	0.0	0.0	0.003948506686736248	7.179103066793179E-4
26	0.0	0.0	0.0	0.0044271135578557936	7.179103066793179E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	255	0.0	18.195057	28
TAGACTG	80	2.789444E-6	18.003298	5
CTAGCGG	250	0.0	17.918995	29
GGACCGT	45	0.008838972	17.781038	6
TAGCGGC	255	0.0	17.567642	30
ATAGAGC	65	2.0985198E-4	17.231863	3
TCGCGTA	215	0.0	17.118391	9
TCTAGAT	290	0.0	17.103512	2
CGCGTAA	215	0.0	16.373135	10
GCGTAAC	215	0.0	16.373135	11
AGCGGCG	275	0.0	16.289995	31
TATACTG	100	1.3108111E-6	16.002934	5
GATATAC	110	2.0144034E-7	16.001974	1
CTATACT	80	5.61486E-5	16.001019	4
TTAAGAC	80	5.61486E-5	16.001019	3
ACTATAC	60	0.0024401222	16.001017	3
TAACGAA	160	1.8189894E-11	15.999104	13
CGAACGA	170	3.6379788E-12	15.999103	16
ACGAACG	170	3.6379788E-12	15.999103	15
GTCGCGT	225	0.0	15.646376	8
>>END_MODULE