FastQCFastQC Report
Thu 2 Feb 2017
SRR4063005_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063005_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970862
Sequences flagged as poor quality0
Sequence length38
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA145410.7377989935368382No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA127990.6494112728339173No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC124020.6292678026163171No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG106910.5424529977238386No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA62950.3194033879591772No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA61600.31255359330079935No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA59770.3032683160972204No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA49510.2512098766935483No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA43310.21975156048470162No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG40270.2043268376984284No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT38230.193976036881324No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT36920.18732919910171286No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA33930.17215817241389808No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA30180.153130965029515No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA28020.14217129357611036No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT27900.14156242293981008No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG27330.13867028741738385No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG27040.13719885004632493No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT26690.13542297735711584No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT26590.13491558516019894No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT24650.1250721765400114No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA24490.12426034902494443No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA24460.12410813136586935No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA23360.11852681719978365No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT22190.11259032849585612No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA22080.11203219707924755No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC21980.11152480488233069No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT21920.11122036956418055No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA19970.10132622172430135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC38050.021.900654
AGTGCGG453.5369472E-421.3342810
GTATCAA29650.019.7133451
AGGACCT60200.019.5022415
CTAGCGG2550.019.4498729
GGACCTG59200.019.2107326
GGTATCA12050.019.0038281
TGTAGGA113100.018.887742
TAGCGGC2550.018.82293330
AAGACGG3150.018.787815
GCATCGT1800.018.66465427
GTCCTAT6600.018.4400371
CTGTAGG112700.018.3866441
TAGGACA25650.018.270664
GGACGTA1159.458745E-1018.0817936
CGGACCA3200.018.0003439
GTAGGAC115000.017.9561563
ACCGTCG2050.017.9492828
GTCCTAG5000.017.9353621
GCGCAAG2950.017.9136491
Produced by FastQC (version 0.11.5)