##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063005_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1970862
Sequences flagged as poor quality	0
Sequence length	38
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.392057891420098	32.0	32.0	32.0	32.0	32.0
2	31.154435977759984	32.0	32.0	32.0	32.0	32.0
3	31.176088432371216	32.0	32.0	32.0	32.0	32.0
4	31.227348743849138	32.0	32.0	32.0	32.0	32.0
5	31.213711056380408	32.0	32.0	32.0	32.0	32.0
6	34.884436353230214	36.0	36.0	36.0	36.0	36.0
7	34.825564144014145	36.0	36.0	36.0	32.0	36.0
8	34.812687544840784	36.0	36.0	36.0	32.0	36.0
9	34.91414924028167	36.0	36.0	36.0	36.0	36.0
10	34.70645687014108	36.0	36.0	36.0	32.0	36.0
11	34.87228126576087	36.0	36.0	36.0	36.0	36.0
12	34.736641124543475	36.0	36.0	36.0	32.0	36.0
13	34.78720326435844	36.0	36.0	36.0	32.0	36.0
14	34.749772434599684	36.0	36.0	36.0	32.0	36.0
15	34.69882213975408	36.0	36.0	36.0	32.0	36.0
16	34.75634519311854	36.0	36.0	36.0	32.0	36.0
17	34.7012205826689	36.0	36.0	36.0	32.0	36.0
18	34.74821474055515	36.0	36.0	36.0	32.0	36.0
19	34.65110697755601	36.0	36.0	36.0	32.0	36.0
20	34.61365889646256	36.0	36.0	36.0	32.0	36.0
21	34.57192893261933	36.0	36.0	36.0	32.0	36.0
22	34.53634551784955	36.0	36.0	36.0	32.0	36.0
23	34.540925239818925	36.0	36.0	36.0	32.0	36.0
24	34.49251292074229	36.0	36.0	36.0	32.0	36.0
25	34.45599083040822	36.0	36.0	36.0	32.0	36.0
26	34.479357255860634	36.0	36.0	36.0	32.0	36.0
27	34.42701924335646	36.0	36.0	36.0	32.0	36.0
28	34.33753758507699	36.0	36.0	36.0	32.0	36.0
29	34.26861951775416	36.0	36.0	36.0	32.0	36.0
30	34.2018467046399	36.0	36.0	36.0	32.0	36.0
31	34.19176329951057	36.0	36.0	36.0	32.0	36.0
32	34.148883077556924	36.0	36.0	36.0	32.0	36.0
33	34.203508921476995	36.0	36.0	36.0	32.0	36.0
34	34.1768317619397	36.0	36.0	36.0	32.0	36.0
35	34.19037304489102	36.0	36.0	36.0	32.0	36.0
36	34.09702099893346	36.0	36.0	36.0	32.0	36.0
37	34.003930259957315	36.0	36.0	36.0	32.0	36.0
38	33.38555870476979	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	13.0
11	24.0
12	12.0
13	12.0
14	113.0
15	304.0
16	434.0
17	515.0
18	663.0
19	817.0
20	1123.0
21	1449.0
22	2143.0
23	3361.0
24	5233.0
25	8320.0
26	12869.0
27	19791.0
28	29371.0
29	41235.0
30	58226.0
31	80997.0
32	113062.0
33	176028.0
34	439719.0
35	975020.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.78315363477443	19.819558024353924	12.37387528583019	26.023413055041456
2	11.219371035337062	19.47780042594346	50.01601730173195	19.28681123698753
3	19.6181106178097	25.17675882195631	29.712887751741455	25.492242808492545
4	10.320598168474378	16.547663505877768	40.46034738910178	32.67139093654608
5	10.24258965253222	40.828745232408814	37.430379342205605	11.498285772853361
6	30.637210753708416	39.70449373495259	18.289321132198967	11.368974379140017
7	27.115800091533554	31.236484340354625	22.459563378866708	19.188152189245113
8	21.509808667185222	46.13820674132447	19.327663724294837	13.02432086719547
9	27.057680403478685	14.113584932466031	20.781993647443247	38.04674101661204
10	15.278285171519379	29.06772488705415	35.870504998843145	19.783484942583325
11	35.243732707132594	19.29347426232744	29.366250817550192	16.096542212989775
12	26.83882235402344	22.372797419136166	34.96922336112051	15.819156865719883
13	32.13133405528733	22.87130983904215	25.605685235499433	19.391670870171087
14	17.804144364674862	26.520899427404604	26.445696806578045	29.229259401342482
15	19.23739419671722	41.90464686901454	21.126145832170288	17.73181310209795
16	17.717257050762242	26.93406906798712	36.950825062746176	18.397848818504464
17	17.94795019840555	32.3761621023325	31.673432433762894	18.002455265499055
18	18.489614386364618	26.175095331484634	39.441178634584205	15.894111647566541
19	24.991804950723143	24.497818543848844	26.695766280239226	23.81461022518879
20	24.65749045540069	28.800333680409672	29.820910639006105	16.721265225183537
21	20.970936253797436	26.83016556750089	25.658928614015974	26.539969564685702
22	23.16425950555052	33.90831864747704	26.549880069030714	16.377541777941733
23	18.106781632292677	32.6803594299621	30.693477771079568	18.519381166665653
24	24.59666533372036	26.43521779463019	31.94478557494981	17.023331296699638
25	20.54771440576986	30.265321367601178	31.548690799770213	17.638273426858753
26	15.68211310198951	33.09283441098571	33.38316872935472	17.841883757670058
27	17.96672421019728	31.52451790801357	31.195139550673318	19.31361833111583
28	16.884561383390004	32.90057150743904	27.375881198709322	22.838985910461634
29	20.14649951412094	28.408016623914385	26.806620133914866	24.638863728049813
30	16.94791412434424	29.652938421546953	30.94911059096216	22.450036863146646
31	24.112407795395974	26.41634948671904	28.748453002481796	20.7227897154032
32	19.115118879926605	31.79860437361219	29.484941605335692	19.601335141125514
33	17.022598084536543	31.992522446361644	33.61347096308009	17.371408506021723
34	19.4244830664195	31.391735862633467	32.66306744456415	16.520713626382882
35	17.751350769813193	32.56254748056754	32.21650073101678	17.46960101860249
36	16.127265927144236	30.77687408242669	34.08691274673936	19.008947243689708
37	17.62160318916593	30.476913458962223	27.916720793719097	23.984762558152752
38	19.293193664144447	34.55406870067873	27.207583704088588	18.945153931088235
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21.0
1	51.0
2	81.0
3	81.0
4	209.0
5	337.0
6	337.0
7	461.0
8	585.0
9	652.5
10	720.0
11	720.0
12	1083.5
13	1447.0
14	2162.5
15	2878.0
16	2878.0
17	4522.0
18	6166.0
19	6166.0
20	8895.5
21	11625.0
22	16972.5
23	22320.0
24	22320.0
25	37476.5
26	52633.0
27	52633.0
28	73351.5
29	94070.0
30	132038.0
31	170006.0
32	170006.0
33	212080.5
34	254155.0
35	254155.0
36	265306.0
37	276457.0
38	275233.5
39	274010.0
40	274010.0
41	234431.5
42	194853.0
43	161797.5
44	128742.0
45	128742.0
46	110695.0
47	92648.0
48	92648.0
49	85909.0
50	79170.0
51	74528.5
52	69887.0
53	69887.0
54	62311.5
55	54736.0
56	54736.0
57	51291.0
58	47846.0
59	41716.5
60	35587.0
61	35587.0
62	34916.0
63	34245.0
64	28649.0
65	23053.0
66	23053.0
67	19725.0
68	16397.0
69	16397.0
70	13097.5
71	9798.0
72	7621.0
73	5444.0
74	5444.0
75	4014.0
76	2584.0
77	2584.0
78	2686.0
79	2788.0
80	2214.5
81	1641.0
82	1641.0
83	1679.0
84	1717.0
85	1717.0
86	1142.0
87	567.0
88	513.0
89	459.0
90	459.0
91	326.5
92	194.0
93	179.5
94	165.0
95	165.0
96	158.5
97	152.0
98	152.0
99	415.0
100	678.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12547809029754492
2	0.05667570839561572
3	0.01212667350631348
4	0.0023340041058176574
5	1.522176590750646E-4
6	3.5517453784181745E-4
7	0.0
8	1.522176590750646E-4
9	1.0147843938337642E-4
10	3.044353181501292E-4
11	0.0017758726892090873
12	0.0024862217648927223
13	0.01440993839243945
14	0.009437494862654007
15	0.023340041058176574
16	0.011365585210938159
17	0.020447905535750346
18	0.005429096507010638
19	0.008118275150670113
20	0.005784271044852456
21	0.006342402461461026
22	0.00735718685529479
23	0.009437494862654007
24	0.01471437371058958
25	0.017555770013324118
26	0.01902720738438308
27	0.009133059544503877
28	0.005530574946394014
29	0.010503018476179458
30	0.002993613961809604
31	0.005530574946394014
32	0.00679905543868622
33	0.008321232029436866
34	0.01273554414261374
35	0.016185811081648536
36	0.015779897324115034
37	0.00964045174142076
38	0.005581314166085703
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1970862.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.69375356511192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.48844858629573	40.991606360157846
2	10.857906800006944	10.791402895049961
3	2.7552668501910214	4.107586555785443
4	1.138590047474491	2.2632325292354576
5	0.618733746105738	1.5373601150698535
6	0.3804862639448578	1.1344674381231534
7	0.2553869757645116	0.8883796206166593
8	0.1828679594361524	0.7269916248940033
9	0.1497723130388157	0.6698473573524936
>10	0.9422371238738902	9.095201093882483
>50	0.10853223725512233	3.7737455494593926
>100	0.10291156582321237	10.484451477980924
>500	0.012197196543213997	4.290495420649129
>1k	0.005944852096692536	5.737604881040443
>5k	3.074923498289243E-4	0.9388307899733451
>10k+	4.099897997718991E-4	2.568796290729477
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	14541	0.7377989935368382	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	12799	0.6494112728339173	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	12402	0.6292678026163171	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	10691	0.5424529977238386	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	6295	0.3194033879591772	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	6160	0.31255359330079935	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	5977	0.3032683160972204	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	4951	0.2512098766935483	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	4331	0.21975156048470162	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	4027	0.2043268376984284	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	3823	0.193976036881324	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	3692	0.18732919910171286	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	3393	0.17215817241389808	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	3018	0.153130965029515	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	2802	0.14217129357611036	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	2790	0.14156242293981008	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	2733	0.13867028741738385	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	2704	0.13719885004632493	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	2669	0.13542297735711584	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	2659	0.13491558516019894	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	2465	0.1250721765400114	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA	2449	0.12426034902494443	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	2446	0.12410813136586935	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA	2336	0.11852681719978365	No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT	2219	0.11259032849585612	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	2208	0.11203219707924755	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC	2198	0.11152480488233069	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2192	0.11122036956418055	No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA	1997	0.10132622172430135	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.0147843938337641E-4	0.0
10	0.0	0.0	0.0	1.522176590750646E-4	0.0
11	0.0	0.0	0.0	1.522176590750646E-4	0.0
12	0.0	0.0	0.0	1.522176590750646E-4	0.0
13	0.0	0.0	0.0	1.522176590750646E-4	0.0
14	0.0	0.0	0.0	1.522176590750646E-4	0.0
15	0.0	0.0	0.0	1.522176590750646E-4	0.0
16	0.0	0.0	0.0	1.522176590750646E-4	0.0
17	0.0	0.0	0.0	4.0591375753350564E-4	0.0
18	0.0	0.0	0.0	4.0591375753350564E-4	0.0
19	0.0	0.0	0.0	4.566529772251938E-4	0.0
20	0.0	0.0	0.0	5.073921969168821E-4	0.0
21	0.0	0.0	0.0	6.088706363002584E-4	0.0
22	0.0	0.0	0.0	9.133059544503876E-4	0.0
23	0.0	0.0	0.0	0.0010655236135254523	0.0
24	0.0	0.0	0.0	0.0013192197119838934	0.0
25	0.0	0.0	0.0	0.001725133469517399	0.0
26	0.0	0.0	0.0	0.002232525666434281	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	3805	0.0	21.90065	4
AGTGCGG	45	3.5369472E-4	21.33428	10
GTATCAA	2965	0.0	19.713345	1
AGGACCT	6020	0.0	19.502241	5
CTAGCGG	255	0.0	19.44987	29
GGACCTG	5920	0.0	19.210732	6
GGTATCA	1205	0.0	19.003828	1
TGTAGGA	11310	0.0	18.88774	2
TAGCGGC	255	0.0	18.822933	30
AAGACGG	315	0.0	18.78781	5
GCATCGT	180	0.0	18.664654	27
GTCCTAT	660	0.0	18.440037	1
CTGTAGG	11270	0.0	18.386644	1
TAGGACA	2565	0.0	18.27066	4
GGACGTA	115	9.458745E-10	18.081793	6
CGGACCA	320	0.0	18.000343	9
GTAGGAC	11500	0.0	17.956156	3
ACCGTCG	205	0.0	17.949282	8
GTCCTAG	500	0.0	17.935362	1
GCGCAAG	295	0.0	17.913649	1
>>END_MODULE