FastQCFastQC Report
Thu 2 Feb 2017
SRR4063005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970862
Sequences flagged as poor quality0
Sequence length38
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA151520.7688006567684597No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC117710.5972513549908618No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA116910.5931922174155269No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG113940.5781226691670954No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA65170.33066749473073204No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA60430.30661710459687186No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA59610.30245648858215335No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA55960.2839366733946872No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG42650.2164027719850502No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT39090.1983396097748092No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA37420.18986616008629728No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT37140.18844546193493No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA36130.1833208007460695No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA31250.15856006153652566No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG31190.15825562621837552No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA30160.15302948659013163No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG29290.14861517447695474No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT29070.1474989116437376No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA28220.14318607796994412No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT27450.13927915805368413No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT27230.13816289522046699No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT26600.13496632437989062No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA26420.13405301842544023No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA25390.12882687879719634No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC25260.12816726894120442No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT24410.12385443526741091No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA24050.12202782335851013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT21270.10792232028422082No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA21140.10726271042822885No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA20550.10426909646641927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGGCG350.002065251622.8605885
TAGGACC38700.021.9964714
AAGACGG3100.021.6806875
GCGCAAG3200.020.5004881
GACGTTA555.8281003E-520.3672227
GGTATCA11250.019.769361
GTATCAA29200.019.7264981
TCTAGGA5700.019.6495912
AGGACCT64900.019.3804245
CAAGACG3400.019.2955554
CTAGGAC5900.019.2556723
GTGTAGG3750.019.2004571
CTAGCGG2250.019.19899629
GGACCTG64950.018.9712946
TAGAAAT30300.018.6944624
TGTAGGA113750.018.6237412
CGCAAGA3450.018.5511672
TTTAGAA31050.018.3965722
AAATGTC29150.018.2810927
ATTTAGA29800.018.2554681
Produced by FastQC (version 0.11.5)