##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063005_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1970862
Sequences flagged as poor quality	0
Sequence length	38
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.312529745867543	32.0	32.0	32.0	32.0	32.0
2	31.394986559180705	32.0	32.0	32.0	32.0	32.0
3	31.45729229139331	32.0	32.0	32.0	32.0	32.0
4	31.531893658713802	32.0	32.0	32.0	32.0	32.0
5	31.497805528748334	32.0	32.0	32.0	32.0	32.0
6	35.05512968437161	36.0	36.0	36.0	36.0	36.0
7	35.080069025634465	36.0	36.0	36.0	36.0	36.0
8	34.989996762837784	36.0	36.0	36.0	36.0	36.0
9	35.11744911617353	36.0	36.0	36.0	36.0	36.0
10	34.998208905544885	36.0	36.0	36.0	36.0	36.0
11	35.11986785477624	36.0	36.0	36.0	36.0	36.0
12	35.05729472687585	36.0	36.0	36.0	36.0	36.0
13	35.0852971948315	36.0	36.0	36.0	36.0	36.0
14	35.050815328521225	36.0	36.0	36.0	36.0	36.0
15	35.02169862730115	36.0	36.0	36.0	36.0	36.0
16	35.05284438991669	36.0	36.0	36.0	36.0	36.0
17	35.06004479258314	36.0	36.0	36.0	36.0	36.0
18	35.07123887923152	36.0	36.0	36.0	36.0	36.0
19	35.01530294865901	36.0	36.0	36.0	36.0	36.0
20	34.998814224435804	36.0	36.0	36.0	36.0	36.0
21	34.962349469419976	36.0	36.0	36.0	36.0	36.0
22	34.929427834115224	36.0	36.0	36.0	36.0	36.0
23	34.91486618545591	36.0	36.0	36.0	36.0	36.0
24	34.90366803966995	36.0	36.0	36.0	36.0	36.0
25	34.86738340888403	36.0	36.0	36.0	32.0	36.0
26	34.817438765372714	36.0	36.0	36.0	32.0	36.0
27	34.75142348880845	36.0	36.0	36.0	32.0	36.0
28	34.681354655983014	36.0	36.0	36.0	32.0	36.0
29	34.65050216605729	36.0	36.0	36.0	32.0	36.0
30	34.57554055027698	36.0	36.0	36.0	32.0	36.0
31	34.57637926957849	36.0	36.0	36.0	32.0	36.0
32	34.55303212502955	36.0	36.0	36.0	32.0	36.0
33	34.600675237535654	36.0	36.0	36.0	32.0	36.0
34	34.57424872974364	36.0	36.0	36.0	32.0	36.0
35	34.56672816260093	36.0	36.0	36.0	32.0	36.0
36	34.49448058768194	36.0	36.0	36.0	32.0	36.0
37	34.403693916672	36.0	36.0	36.0	32.0	36.0
38	34.02585011025632	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	8.0
21	16.0
22	88.0
23	288.0
24	848.0
25	2171.0
26	4873.0
27	10094.0
28	18804.0
29	31114.0
30	49309.0
31	73094.0
32	105719.0
33	170856.0
34	442077.0
35	1061502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.39847403546376	21.073351032735776	12.87367660431281	26.65449832748766
2	10.691311720455314	20.496513708214984	48.93564338852745	19.876531182802246
3	19.17044420157271	25.920333336377688	30.08059417655828	24.828628285491323
4	10.094547749810234	17.442979091318698	40.66471624512394	31.797756913747122
5	9.954583301409892	41.591619094395796	36.706799718498665	11.746997885695642
6	31.087276068682673	39.79463797802077	17.78603361677967	11.332052336516883
7	27.218874264087685	31.39640551578428	22.508274336036223	18.87644588409181
8	21.60055611510974	46.23797768943305	19.05216877281734	13.109297422639873
9	26.492353281441865	14.10984483301028	20.74594830473214	38.65185358081572
10	15.275493786161556	29.543753612033026	35.20189076451041	19.978861837295007
11	34.84421793353447	19.323501623226704	29.6461375931998	16.18614285003902
12	26.792825046578745	22.499868077627184	34.71648867294211	15.990818202851958
13	31.994676424863105	22.827745913450805	25.675239591324782	19.502338070361304
14	17.864264469049584	26.7370318165351	26.28261136497634	29.116092349438976
15	19.262840320631277	41.93327589653664	20.93556017620716	17.86832360662492
16	17.839208345997005	27.3633198891246	36.199458003380244	18.598013761498148
17	18.084574160950893	32.973084873522346	30.889580295322556	18.052760670204204
18	18.704962600121167	26.49688308973434	38.84011158569194	15.958042724452548
19	25.31704401424351	24.68508703298354	26.78000793561396	23.217861017158988
20	25.02569941477384	29.08980943363868	29.221274751859845	16.66321639972763
21	21.19526501121338	26.79896085972621	25.718011426483873	26.28776270257654
22	23.478075560922644	33.58376546279289	26.478796058573415	16.45936291771105
23	18.098411960679055	32.88917821578216	30.252813749138703	18.759596074400083
24	24.86267430190445	26.687764034214474	31.261346558003556	17.18821510587753
25	20.70261641860262	30.72452561366549	30.84198690725175	17.730871060480137
26	15.852200712175687	33.43673986306499	32.67894961696963	18.032109807789688
27	18.264421757213476	31.845657147467172	30.62821845702813	19.261702638291222
28	17.066542457056862	33.07638992481463	27.431296559576467	22.425771058552044
29	20.147275659077092	28.223437257403106	26.916547175804297	24.71273990771551
30	17.092419459099624	29.451884505358567	30.944023478051736	22.511672557490073
31	23.88142853228689	26.484502720129566	28.879495368016634	20.75457337956691
32	19.243559417148433	31.824501157361603	29.323717236417362	19.6082221890726
33	17.15756861718375	32.19327380608079	33.048026701006975	17.601130875728487
34	19.72974262023419	31.599168282710817	31.988135140867296	16.6829539561877
35	18.016025475147423	32.93173240947362	31.38235959696823	17.669882518410727
36	16.335187344420866	30.907237543775263	33.626656762370985	19.13091834943289
37	17.814134119994197	30.542422554192022	27.587471877787483	24.055971448026295
38	19.53064192216401	34.2101070496057	27.30044011199161	18.95881091623868
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	22.5
2	39.0
3	39.0
4	120.5
5	202.0
6	202.0
7	333.5
8	465.0
9	544.0
10	623.0
11	623.0
12	945.0
13	1267.0
14	1981.5
15	2696.0
16	2696.0
17	4343.0
18	5990.0
19	5990.0
20	8485.5
21	10981.0
22	16207.5
23	21434.0
24	21434.0
25	36103.0
26	50772.0
27	50772.0
28	71189.5
29	91607.0
30	129380.0
31	167153.0
32	167153.0
33	209153.5
34	251154.0
35	251154.0
36	264039.0
37	276924.0
38	279081.5
39	281239.0
40	281239.0
41	239937.0
42	198635.0
43	164470.0
44	130305.0
45	130305.0
46	111753.5
47	93202.0
48	93202.0
49	86390.5
50	79579.0
51	74568.0
52	69557.0
53	69557.0
54	62216.0
55	54875.0
56	54875.0
57	51296.0
58	47717.0
59	41679.5
60	35642.0
61	35642.0
62	35189.5
63	34737.0
64	28958.0
65	23179.0
66	23179.0
67	19902.5
68	16626.0
69	16626.0
70	13256.0
71	9886.0
72	7541.0
73	5196.0
74	5196.0
75	3874.5
76	2553.0
77	2553.0
78	2668.0
79	2783.0
80	2181.0
81	1579.0
82	1579.0
83	1518.5
84	1458.0
85	1458.0
86	933.5
87	409.0
88	350.5
89	292.0
90	292.0
91	171.0
92	50.0
93	37.5
94	25.0
95	25.0
96	16.5
97	8.0
98	8.0
99	12.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008828624226353748
2	0.0
3	0.0
4	3.044353181501292E-4
5	5.073921969168821E-5
6	5.073921969168821E-5
7	4.566529772251938E-4
8	0.0023847433255093455
9	0.004160616014718433
10	9.640451741420759E-4
11	0.0048709650904020674
12	3.044353181501292E-4
13	3.044353181501292E-4
14	0.0
15	0.0
16	5.073921969168821E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0147843938337642E-4
22	1.0147843938337642E-4
23	2.0295687876675285E-4
24	0.0
25	0.0
26	0.0
27	1.522176590750646E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1970862.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.203884431355654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.52291753935019	39.297212955737734
2	11.430135518952197	11.019538631806103
3	2.984499051044193	4.3159334202607464
4	1.1813698455961485	2.2778646203122093
5	0.6183018433349553	1.4902275299906178
6	0.40483539784371414	1.1708783238828173
7	0.2677699798040921	0.9035287212464025
8	0.1949694929113358	0.7518629523150436
9	0.1627466047888752	0.7060516675954608
>10	0.9891470121171311	9.297139112668004
>50	0.11600411610451827	3.941122280925454
>100	0.10719804973734494	10.61312379121477
>500	0.01227496640091573	4.17174752917015
>1k	0.006984032607417571	6.262747669333652
>5k	4.232747034798528E-4	1.2301771126856578
>10k+	4.232747034798528E-4	2.5508436808551798
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	15152	0.7688006567684597	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	11771	0.5972513549908618	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	11691	0.5931922174155269	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	11394	0.5781226691670954	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	6517	0.33066749473073204	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	6043	0.30661710459687186	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	5961	0.30245648858215335	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	5596	0.2839366733946872	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	4265	0.2164027719850502	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	3909	0.1983396097748092	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	3742	0.18986616008629728	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	3714	0.18844546193493	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	3613	0.1833208007460695	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	3125	0.15856006153652566	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	3119	0.15825562621837552	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	3016	0.15302948659013163	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	2929	0.14861517447695474	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	2907	0.1474989116437376	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA	2822	0.14318607796994412	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	2745	0.13927915805368413	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	2723	0.13816289522046699	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	2660	0.13496632437989062	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA	2642	0.13405301842544023	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	2539	0.12882687879719634	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC	2526	0.12816726894120442	No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT	2441	0.12385443526741091	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	2405	0.12202782335851013	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2127	0.10792232028422082	No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA	2114	0.10726271042822885	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA	2055	0.10426909646641927	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.0739219691688205E-5	0.0
8	0.0	0.0	0.0	1.0147843938337641E-4	0.0
9	0.0	0.0	0.0	1.0147843938337641E-4	0.0
10	0.0	0.0	0.0	2.0295687876675282E-4	0.0
11	0.0	0.0	0.0	2.0295687876675282E-4	0.0
12	0.0	0.0	0.0	2.0295687876675282E-4	0.0
13	0.0	0.0	0.0	2.0295687876675282E-4	0.0
14	0.0	0.0	0.0	2.0295687876675282E-4	0.0
15	0.0	0.0	0.0	2.0295687876675282E-4	0.0
16	0.0	0.0	0.0	2.0295687876675282E-4	0.0
17	0.0	0.0	0.0	3.044353181501292E-4	0.0
18	0.0	0.0	0.0	3.044353181501292E-4	0.0
19	0.0	0.0	0.0	3.5517453784181745E-4	0.0
20	0.0	0.0	0.0	3.5517453784181745E-4	0.0
21	0.0	0.0	0.0	4.0591375753350564E-4	0.0
22	0.0	0.0	0.0	4.0591375753350564E-4	0.0
23	0.0	0.0	0.0	5.073921969168821E-4	0.0
24	0.0	0.0	0.0	8.625667347586995E-4	0.0
25	0.0	0.0	0.0	0.0010655236135254523	0.0
26	0.0	0.0	0.0	0.0013699589316755816	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAGGCG	35	0.0020652516	22.860588	5
TAGGACC	3870	0.0	21.996471	4
AAGACGG	310	0.0	21.680687	5
GCGCAAG	320	0.0	20.500488	1
GACGTTA	55	5.8281003E-5	20.367222	7
GGTATCA	1125	0.0	19.76936	1
GTATCAA	2920	0.0	19.726498	1
TCTAGGA	570	0.0	19.649591	2
AGGACCT	6490	0.0	19.380424	5
CAAGACG	340	0.0	19.295555	4
CTAGGAC	590	0.0	19.255672	3
GTGTAGG	375	0.0	19.200457	1
CTAGCGG	225	0.0	19.198996	29
GGACCTG	6495	0.0	18.971294	6
TAGAAAT	3030	0.0	18.694462	4
TGTAGGA	11375	0.0	18.623741	2
CGCAAGA	345	0.0	18.551167	2
TTTAGAA	3105	0.0	18.396572	2
AAATGTC	2915	0.0	18.281092	7
ATTTAGA	2980	0.0	18.255468	1
>>END_MODULE