FastQCFastQC Report
Thu 2 Feb 2017
SRR4063004_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063004_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933931
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33740.36126865903369737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22570.24166667558952426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13410.14358662470782105No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13110.1403743959671539No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12450.1333074927376862No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11260.12056565206637321No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10400.11135726300979408No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA10360.1109289658443718No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10260.10985822293081608No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.10889455430861594No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10130.10846625714319366No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10050.10760966281234909No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT9930.10632477131608223No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9920.10621769702472666No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9870.10568232556794882No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG9530.10204179966185939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG2450.022.20218728
CTAGCGG2600.020.30595829
GTACCGT400.00449063819.994016
TAGCGGC2750.019.78012830
TACCGTC2250.019.1973327
CGTTTAT2050.018.7301131
ATACCGT2400.018.6610766
TAATACC601.140977E-418.6600784
ATCGTTT2100.018.28415529
ACACCGT701.7912886E-518.2802376
AGCGGCG3000.018.13178431
ACCGTCG2300.018.0844448
CGTCGTA2400.017.33556710
GTACTAA652.0841036E-417.2477721
GTATAGG753.2053948E-517.0835081
AATCGCT1051.18661774E-716.76406515
CGGTCCA3350.016.7185710
CGCCGGT3250.016.2438987
CAATTAG600.0024410916.00034923
CCGTCGT2600.016.0003479
Produced by FastQC (version 0.11.5)