##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063004_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	933931
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.241817650340337	32.0	32.0	32.0	32.0	32.0
2	30.927597434928277	32.0	32.0	32.0	32.0	32.0
3	30.98169993286442	32.0	32.0	32.0	32.0	32.0
4	31.038575654946673	32.0	32.0	32.0	32.0	32.0
5	30.980771598758366	32.0	32.0	32.0	32.0	32.0
6	34.607808285622816	36.0	36.0	36.0	32.0	36.0
7	34.5353511126625	36.0	36.0	36.0	32.0	36.0
8	34.52247864135573	36.0	36.0	36.0	32.0	36.0
9	34.62332549192606	36.0	36.0	36.0	32.0	36.0
10	34.414028445356244	36.0	36.0	36.0	32.0	36.0
11	34.63783619989057	36.0	36.0	36.0	32.0	36.0
12	34.48092953333811	36.0	36.0	36.0	32.0	36.0
13	34.55431932337614	36.0	36.0	36.0	32.0	36.0
14	34.473117393040816	36.0	36.0	36.0	32.0	36.0
15	34.42876936304717	36.0	36.0	36.0	32.0	36.0
16	34.44706193498235	36.0	36.0	36.0	32.0	36.0
17	34.36388662545734	36.0	36.0	36.0	32.0	36.0
18	34.39704110903268	36.0	36.0	36.0	32.0	36.0
19	34.37901086911132	36.0	36.0	36.0	32.0	36.0
20	34.34502441829214	36.0	36.0	36.0	32.0	36.0
21	34.324194185651834	36.0	36.0	36.0	32.0	36.0
22	34.300222393303144	36.0	36.0	36.0	32.0	36.0
23	34.25600392320204	36.0	36.0	36.0	32.0	36.0
24	34.24598391101698	36.0	36.0	36.0	32.0	36.0
25	34.21902795816822	36.0	36.0	36.0	32.0	36.0
26	34.175219582603	36.0	36.0	36.0	32.0	36.0
27	34.17408138288589	36.0	36.0	36.0	32.0	36.0
28	34.16508285944037	36.0	36.0	36.0	32.0	36.0
29	34.12650399226495	36.0	36.0	36.0	32.0	36.0
30	34.10117663938771	36.0	36.0	36.0	32.0	36.0
31	34.11312398881716	36.0	36.0	36.0	32.0	36.0
32	34.06470071129452	36.0	36.0	36.0	32.0	36.0
33	34.01437686510031	36.0	36.0	36.0	32.0	36.0
34	34.018228327360376	36.0	36.0	36.0	32.0	36.0
35	33.973435939057595	36.0	36.0	36.0	32.0	36.0
36	33.94002233569718	36.0	36.0	36.0	32.0	36.0
37	33.93593638073905	36.0	36.0	36.0	32.0	36.0
38	33.30336823598317	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	0.0
8	3.0
9	4.0
10	7.0
11	10.0
12	4.0
13	9.0
14	122.0
15	226.0
16	340.0
17	390.0
18	521.0
19	701.0
20	926.0
21	1298.0
22	1789.0
23	2488.0
24	3745.0
25	5464.0
26	8128.0
27	11475.0
28	17008.0
29	22583.0
30	30759.0
31	41869.0
32	58682.0
33	89005.0
34	201891.0
35	434481.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.40092024605647	16.91906251541069	11.29156062324586	25.388456615286977
2	16.60747478318629	19.550570224072082	37.71267563383526	26.12927935890637
3	19.344402739329947	23.833348504238035	28.037866578139976	28.784382178292038
4	12.313952243280864	15.02944640753828	35.86497483670629	36.791626512474565
5	14.429561102009785	36.76806612915315	33.488805370852205	15.313567397984858
6	34.568584663024694	35.25675481783354	16.79115904257939	13.383501476562381
7	30.62909493119927	30.39535125261128	20.476074733732435	18.499479082457018
8	28.57828136276322	32.766514691742174	19.228932485014873	19.42627146047974
9	27.65587925407526	13.99283456540547	18.4126613614754	39.938624819043866
10	15.848364859742633	26.750727573704985	31.614710373198733	25.78619719335365
11	37.88808569553201	21.325265469621836	21.63043373512037	19.156215099725777
12	24.86749031474664	23.83643111530859	28.594037438938468	22.702041131006304
13	29.82871915252713	19.354071802164945	25.21193247289535	25.605276572412585
14	23.671506896256318	19.755311402381565	25.24865073245952	31.324530968902597
15	25.199690690954423	26.999637997031144	22.783909931947726	25.016761380066704
16	25.70822151639827	25.803207905609014	24.004784658252465	24.48378591974025
17	23.827781532170743	25.859437669282364	25.363786398720816	24.94899439982607
18	24.919100435390643	24.79809910116354	26.575962067687932	23.706838395757888
19	25.2747735206562	25.143811706251473	25.81736020388495	23.764054569207378
20	25.624524158047524	24.14177118681274	25.65043780136657	24.583266853773168
21	26.900602329005487	24.162227278811404	24.705768973363675	24.231401418819434
22	25.550538143338557	24.406361131605518	25.36742698844258	24.675673736613344
23	24.165426255314138	24.201514194232352	25.909212597582	25.72384695287151
24	24.46802814337271	25.273883849687834	25.531008042493493	24.727079964445966
25	24.742784746683004	24.629157023660483	25.572663375996385	25.05539485366013
26	24.323126006033792	25.71601761087925	26.025525279493994	23.935331103592965
27	25.19034187649784	24.984419272556718	25.21978998678583	24.605448864159616
28	24.1420961049391	24.97154330076295	26.024253781287644	24.862106813010307
29	24.2304467344384	25.25312666988641	25.978319185368505	24.538107410306687
30	24.122522885237053	25.470099014989845	26.261940531042438	24.145437568730664
31	24.584500956764114	25.43964569209864	24.99922366649998	24.97662968463727
32	24.266997906549662	25.324431261477837	25.003506930873304	25.405063901099194
33	23.89883160982129	24.959495592970597	25.625770333319416	25.5159024638887
34	24.78866059973913	25.14408774413529	25.793473162519838	24.27377849360574
35	25.42192093299706	25.005756266298267	25.69500891551943	23.877313885185245
36	24.11306731778363	25.842275482447686	25.231210777699243	24.813446422069436
37	25.19869613359062	25.595017551224085	25.165178542745103	24.04110777244019
38	24.136686680094616	25.45710176865591	25.65338113426558	24.752830416983894
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	99.5
2	131.0
3	131.0
4	417.0
5	703.0
6	703.0
7	921.5
8	1140.0
9	1128.5
10	1117.0
11	1117.0
12	1424.0
13	1731.0
14	2204.5
15	2678.0
16	2678.0
17	3902.5
18	5127.0
19	5127.0
20	6116.5
21	7106.0
22	7273.5
23	7441.0
24	7441.0
25	8609.0
26	9777.0
27	9777.0
28	12590.0
29	15403.0
30	18893.5
31	22384.0
32	22384.0
33	27521.5
34	32659.0
35	32659.0
36	36323.0
37	39987.0
38	46087.5
39	52188.0
40	52188.0
41	55645.5
42	59103.0
43	65951.0
44	72799.0
45	72799.0
46	76038.0
47	79277.0
48	79277.0
49	82273.0
50	85269.0
51	85622.0
52	85975.0
53	85975.0
54	81841.5
55	77708.0
56	77708.0
57	74894.5
58	72081.0
59	65200.0
60	58319.0
61	58319.0
62	54581.5
63	50844.0
64	42420.5
65	33997.0
66	33997.0
67	28306.5
68	22616.0
69	22616.0
70	18254.0
71	13892.0
72	10964.5
73	8037.0
74	8037.0
75	6156.0
76	4275.0
77	4275.0
78	4026.5
79	3778.0
80	2988.5
81	2199.0
82	2199.0
83	2094.5
84	1990.0
85	1990.0
86	1346.5
87	703.0
88	581.5
89	460.0
90	460.0
91	338.0
92	216.0
93	176.5
94	137.0
95	137.0
96	133.5
97	130.0
98	130.0
99	308.0
100	486.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12174346927128449
2	0.05632107725303047
3	0.013491360710801977
4	0.0033193030320227083
5	1.0707429135557123E-4
6	5.353714567778562E-4
7	2.1414858271114246E-4
8	5.353714567778562E-4
9	3.212228740667137E-4
10	5.353714567778562E-4
11	0.0021414858271114248
12	0.003961748780156135
13	0.015311623663846686
14	0.009743760513356983
15	0.025376607051270386
16	0.01209939492317955
17	0.02248560118466996
18	0.006103234607267561
19	0.007602274686245557
20	0.006424457481334275
21	0.005996160315911989
22	0.00770934897760113
23	0.010814503426912696
24	0.015097475081135545
25	0.019273372444002825
26	0.020344115357558534
27	0.009101314765223556
28	0.005996160315911989
29	0.011778172049112837
30	0.0034263773233782794
31	0.0057820117332008465
32	0.007066903229467702
33	0.008780091891156841
34	0.013384286419446405
35	0.017774332365024823
36	0.016168217994691255
37	0.009957909096068125
38	0.006210308898623132
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	933931.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.47401489868075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.2548886447613	44.976226358636126
2	13.608150857645022	15.6423013027157
3	4.162618698374364	7.177272272636857
4	1.6233716420034103	3.7320674375439933
5	0.7081698836622919	2.035068322220179
6	0.40039427129909544	1.380735978839438
7	0.2435175150815906	0.9797150502922337
8	0.16660508431306068	0.7660370478403848
9	0.11146356786428067	0.5765632884082602
>10	0.5632715989538429	6.11694843696708
>50	0.06877855595696676	2.811617773978322
>100	0.07942550335012293	9.2105769882447
>500	0.00710157431841109	2.791357321271966
>1k	0.0022426024163403444	1.803512420404709
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3374	0.36126865903369737	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2257	0.24166667558952426	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1341	0.14358662470782105	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1311	0.1403743959671539	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1245	0.1333074927376862	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1126	0.12056565206637321	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1040	0.11135726300979408	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1036	0.1109289658443718	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1026	0.10985822293081608	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1017	0.10889455430861594	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1013	0.10846625714319366	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1005	0.10760966281234909	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	993	0.10632477131608223	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	992	0.10621769702472666	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	987	0.10568232556794882	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	953	0.10204179966185939	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.0707429135557123E-4	0.0
9	0.0	0.0	0.0	1.0707429135557123E-4	0.0
10	0.0	0.0	0.0	1.0707429135557123E-4	0.0
11	0.0	0.0	0.0	1.0707429135557123E-4	0.0
12	0.0	0.0	0.0	1.0707429135557123E-4	1.0707429135557123E-4
13	0.0	0.0	0.0	1.0707429135557123E-4	1.0707429135557123E-4
14	0.0	0.0	0.0	1.0707429135557123E-4	1.0707429135557123E-4
15	0.0	0.0	0.0	1.0707429135557123E-4	2.1414858271114246E-4
16	0.0	0.0	0.0	1.0707429135557123E-4	2.1414858271114246E-4
17	0.0	0.0	0.0	3.212228740667137E-4	2.1414858271114246E-4
18	0.0	0.0	0.0	4.2829716542228493E-4	2.1414858271114246E-4
19	0.0	0.0	0.0	5.353714567778562E-4	2.1414858271114246E-4
20	0.0	0.0	0.0	5.353714567778562E-4	3.212228740667137E-4
21	0.0	0.0	0.0	6.424457481334274E-4	3.212228740667137E-4
22	0.0	0.0	0.0	6.424457481334274E-4	3.212228740667137E-4
23	0.0	0.0	0.0	0.001391965787622426	4.2829716542228493E-4
24	0.0	0.0	0.0	0.0033193030320227083	4.2829716542228493E-4
25	0.0	0.0	0.0	0.0038546744888005645	4.2829716542228493E-4
26	0.0	0.0	0.0	0.004604194528289563	4.2829716542228493E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	245	0.0	22.202187	28
CTAGCGG	260	0.0	20.305958	29
GTACCGT	40	0.004490638	19.99401	6
TAGCGGC	275	0.0	19.780128	30
TACCGTC	225	0.0	19.197332	7
CGTTTAT	205	0.0	18.73011	31
ATACCGT	240	0.0	18.661076	6
TAATACC	60	1.140977E-4	18.660078	4
ATCGTTT	210	0.0	18.284155	29
ACACCGT	70	1.7912886E-5	18.280237	6
AGCGGCG	300	0.0	18.131784	31
ACCGTCG	230	0.0	18.084444	8
CGTCGTA	240	0.0	17.335567	10
GTACTAA	65	2.0841036E-4	17.247772	1
GTATAGG	75	3.2053948E-5	17.083508	1
AATCGCT	105	1.18661774E-7	16.764065	15
CGGTCCA	335	0.0	16.71857	10
CGCCGGT	325	0.0	16.243898	7
CAATTAG	60	0.00244109	16.000349	23
CCGTCGT	260	0.0	16.000347	9
>>END_MODULE