FastQCFastQC Report
Thu 2 Feb 2017
SRR4063004_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063004_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933931
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39060.41823218203486123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28390.30398391315846673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17410.18641634125004952No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13600.1456210362435769No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12850.13759046439190903No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12540.13427116135988632No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11020.1179958690738395No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10730.11489071462452793No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10710.1146765660418168No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10620.11371289741961665No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10450.11189263446657195No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10110.10825210856048252No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG9590.10268424540999281No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9490.10161350249643711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTA400.00448394719.99916315
TATCCCC701.784849E-518.2878885
TCGCGTA1006.8008376E-817.602099
CTAGCGG2100.017.52307529
GTCCTAT2750.017.4575541
GTCTTAC550.001362811217.4575541
TCTAGCG2150.017.11556428
CGTTATT1601.8189894E-1216.9992892
ATCGTTT2700.016.59189829
GGACGTG1950.016.4122076
CCTATTC2650.016.3020763
CATCGTT2750.016.29022828
GTATAGA1203.101013E-816.0027581
GTCGCGT1203.103014E-816.0019028
ACACGCT2800.016.0019029
ACCGTCG1703.6379788E-1216.0019028
CGCGTAA1102.0164771E-716.00104310
TAGGACG1800.016.0001894
AACGAAC1304.798494E-915.999330514
CGAGCCG2950.015.72815615
Produced by FastQC (version 0.11.5)