##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063004_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	933931
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.132882407801006	32.0	32.0	32.0	32.0	32.0
2	31.241784457310015	32.0	32.0	32.0	32.0	32.0
3	31.350681153104457	32.0	32.0	32.0	32.0	32.0
4	31.449957223820604	32.0	32.0	32.0	32.0	32.0
5	31.34695389702237	32.0	32.0	32.0	32.0	32.0
6	34.87668789236036	36.0	36.0	36.0	36.0	36.0
7	34.92003584847274	36.0	36.0	36.0	36.0	36.0
8	34.849965361466744	36.0	36.0	36.0	32.0	36.0
9	34.97017231465708	36.0	36.0	36.0	36.0	36.0
10	34.8020710309434	36.0	36.0	36.0	32.0	36.0
11	34.995379744328005	36.0	36.0	36.0	36.0	36.0
12	34.89158513851666	36.0	36.0	36.0	32.0	36.0
13	34.94692648600378	36.0	36.0	36.0	36.0	36.0
14	34.889137420216265	36.0	36.0	36.0	32.0	36.0
15	34.85577092954404	36.0	36.0	36.0	32.0	36.0
16	34.870000032122284	36.0	36.0	36.0	32.0	36.0
17	34.83545037053059	36.0	36.0	36.0	32.0	36.0
18	34.82665850046738	36.0	36.0	36.0	32.0	36.0
19	34.8216913240914	36.0	36.0	36.0	32.0	36.0
20	34.80178728407131	36.0	36.0	36.0	32.0	36.0
21	34.78954226811189	36.0	36.0	36.0	32.0	36.0
22	34.78117548298536	36.0	36.0	36.0	32.0	36.0
23	34.72349242074629	36.0	36.0	36.0	32.0	36.0
24	34.69118061184392	36.0	36.0	36.0	32.0	36.0
25	34.67566982999815	36.0	36.0	36.0	32.0	36.0
26	34.609982964480245	36.0	36.0	36.0	32.0	36.0
27	34.592950656954315	36.0	36.0	36.0	32.0	36.0
28	34.56546468636334	36.0	36.0	36.0	32.0	36.0
29	34.52386846565753	36.0	36.0	36.0	32.0	36.0
30	34.497265857970234	36.0	36.0	36.0	32.0	36.0
31	34.50448801892217	36.0	36.0	36.0	32.0	36.0
32	34.46927342598115	36.0	36.0	36.0	32.0	36.0
33	34.41927615637558	36.0	36.0	36.0	32.0	36.0
34	34.39993961009967	36.0	36.0	36.0	32.0	36.0
35	34.37093318457145	36.0	36.0	36.0	32.0	36.0
36	34.331908888344	36.0	36.0	36.0	32.0	36.0
37	34.32655303229039	36.0	36.0	36.0	32.0	36.0
38	33.88307166161098	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	15.0
22	67.0
23	205.0
24	632.0
25	1465.0
26	3243.0
27	6394.0
28	11306.0
29	17812.0
30	26730.0
31	38895.0
32	56752.0
33	90684.0
34	217354.0
35	462373.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.48381058648542	18.220572660949124	12.062202642605726	26.233414109959725
2	15.684563420638142	20.66512408304254	36.7718814344957	26.878431061823626
3	18.293856826682056	24.43349669301051	28.537868429252267	28.734778051055166
4	11.83684876077569	15.887040905591526	35.90961216620928	36.36649816742351
5	13.999856520449583	37.21088602905353	33.19238787447895	15.59686957601793
6	34.647140515265114	35.88706611972884	16.226321757482115	13.239471607523928
7	30.326963106363657	30.612209934812963	20.509396869994262	18.551430088829118
8	28.460496517311718	33.062356449531805	18.993687794797115	19.483459238359362
9	27.46983049395539	13.861268457741277	18.31264924134534	40.35625180695799
10	15.565292250761303	27.083467177200717	31.631374715715626	25.719865856322354
11	38.00532185440392	21.166096467980534	21.568822713717427	19.259758963898125
12	24.65915503212771	23.920019616052183	28.567375036003806	22.8534503158163
13	29.85987157481993	19.29129516269438	25.17332688030889	25.675506382176806
14	23.392306283868937	19.603589558543405	25.340737163666265	31.663366993921393
15	25.161066502771618	27.146759235960687	22.67726416619643	25.014910095071265
16	25.649111122770314	25.93200140053173	23.873712297803586	24.54517517889437
17	23.873926446386296	26.012307119048412	25.287628315153903	24.826138119411393
18	24.96297906376381	24.706857358841287	26.579372566067516	23.750791011327387
19	25.309257322007728	25.08322349295612	25.801906136534715	23.805613048501442
20	25.650503088557937	24.030683208930853	25.719458932190918	24.599354770320293
21	26.818255310081796	24.157994541352625	24.81264675870059	24.21110338986499
22	25.422648996553278	24.406835194462975	25.56066775811061	24.609848050873136
23	24.122981248079356	24.072442182559524	26.105568826819116	25.69900774254201
24	24.43649477316847	25.07251606382056	25.698151148211164	24.79283801479981
25	24.628050680403586	24.614559319692784	25.712177880378746	25.04521211952489
26	24.337129830790495	25.454557135377236	26.087152048705953	24.12116098512631
27	25.30885612412065	24.6855760067671	25.32448898739734	24.681078881714903
28	24.230376762308993	24.745511178020646	26.283633373343427	24.740478686326934
29	24.199860589272653	25.028401455782067	26.17516711620023	24.596570838745045
30	24.13861409461727	25.191154378642533	26.42647047801176	24.24376104872844
31	24.613167353905162	25.082152750042564	25.281096783381212	25.023583112671066
32	24.398269251154527	25.077762704096983	25.229915272113253	25.29405277263524
33	23.970079160023598	24.589825158389644	25.990678112194583	25.449417569392168
34	24.841449743075238	24.91683004418956	25.917439296907375	24.324280915827828
35	25.434534242893747	24.69829141553284	25.87300346599481	23.994170875578604
36	24.371286529732924	25.449417569392168	25.445027523446594	24.73426837742831
37	25.364935953512624	25.27681381172699	25.240836849831517	24.117413384928867
38	24.249007689004863	25.052707319919783	25.813577234292467	24.88470775678289
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	49.0
2	66.0
3	66.0
4	311.0
5	556.0
6	556.0
7	805.0
8	1054.0
9	1137.0
10	1220.0
11	1220.0
12	1539.5
13	1859.0
14	2340.5
15	2822.0
16	2822.0
17	4228.5
18	5635.0
19	5635.0
20	6545.0
21	7455.0
22	7632.0
23	7809.0
24	7809.0
25	8800.0
26	9791.0
27	9791.0
28	12696.5
29	15602.0
30	18925.5
31	22249.0
32	22249.0
33	27427.0
34	32605.0
35	32605.0
36	36175.5
37	39746.0
38	45618.5
39	51491.0
40	51491.0
41	54942.0
42	58393.0
43	65420.0
44	72447.0
45	72447.0
46	75881.5
47	79316.0
48	79316.0
49	82922.5
50	86529.0
51	86430.0
52	86331.0
53	86331.0
54	82199.0
55	78067.0
56	78067.0
57	75228.5
58	72390.0
59	65390.5
60	58391.0
61	58391.0
62	54589.5
63	50788.0
64	42262.0
65	33736.0
66	33736.0
67	28280.5
68	22825.0
69	22825.0
70	18340.0
71	13855.0
72	10985.5
73	8116.0
74	8116.0
75	6104.5
76	4093.0
77	4093.0
78	3927.0
79	3761.0
80	2945.5
81	2130.0
82	2130.0
83	1966.5
84	1803.0
85	1803.0
86	1164.0
87	525.0
88	419.0
89	313.0
90	313.0
91	190.5
92	68.0
93	49.0
94	30.0
95	30.0
96	23.0
97	16.0
98	16.0
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008565943308445699
2	0.0
3	0.0
4	0.0
5	0.0
6	4.2829716542228493E-4
7	3.212228740667137E-4
8	0.002783931575244852
9	0.004390045945578421
10	7.495200394889986E-4
11	0.004925417402356277
12	2.1414858271114246E-4
13	2.1414858271114246E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	1.0707429135557123E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	933931.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.8341905210052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.88215703347488	42.92653003472877
2	14.524145902157414	16.21887858911868
3	4.334376156397003	7.2601915231791745
4	1.7449230620082696	3.89705466754662
5	0.8016174964797779	2.2378832011711567
6	0.405322100218633	1.357849881958867
7	0.2652874044912296	1.0368475239630401
8	0.17126597758033987	0.7649997777589522
9	0.12008333339573654	0.6034280143693447
>10	0.5830321755236333	6.1327209046167805
>50	0.07290450466819764	2.878212452598062
>100	0.08562779478903874	9.913274560525842
>500	0.006942794111926101	2.7532506664960885
>1k	0.002314264703975367	2.0188782019686013
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3906	0.41823218203486123	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2839	0.30398391315846673	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1741	0.18641634125004952	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1360	0.1456210362435769	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1285	0.13759046439190903	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1254	0.13427116135988632	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1102	0.1179958690738395	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1073	0.11489071462452793	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1071	0.1146765660418168	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1062	0.11371289741961665	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1045	0.11189263446657195	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1011	0.10825210856048252	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	959	0.10268424540999281	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	949	0.10161350249643711	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	3.212228740667137E-4
13	0.0	0.0	0.0	0.0	4.2829716542228493E-4
14	0.0	0.0	0.0	0.0	6.424457481334274E-4
15	0.0	0.0	0.0	1.0707429135557123E-4	8.565943308445699E-4
16	0.0	0.0	0.0	1.0707429135557123E-4	9.636686222001411E-4
17	0.0	0.0	0.0	2.1414858271114246E-4	9.636686222001411E-4
18	0.0	0.0	0.0	3.212228740667137E-4	9.636686222001411E-4
19	0.0	0.0	0.0	4.2829716542228493E-4	9.636686222001411E-4
20	0.0	0.0	0.0	4.2829716542228493E-4	9.636686222001411E-4
21	0.0	0.0	0.0	5.353714567778562E-4	0.0011778172049112835
22	0.0	0.0	0.0	5.353714567778562E-4	0.0011778172049112835
23	0.0	0.0	0.0	0.001284891496266855	0.0011778172049112835
24	0.0	0.0	0.0	0.0029980801579559944	0.0011778172049112835
25	0.0	0.0	0.0	0.0034263773233782794	0.0011778172049112835
26	0.0	0.0	0.0	0.004175897362867278	0.0011778172049112835
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCGTA	40	0.004483947	19.999163	15
TATCCCC	70	1.784849E-5	18.287888	5
TCGCGTA	100	6.8008376E-8	17.60209	9
CTAGCGG	210	0.0	17.523075	29
GTCCTAT	275	0.0	17.457554	1
GTCTTAC	55	0.0013628112	17.457554	1
TCTAGCG	215	0.0	17.115564	28
CGTTATT	160	1.8189894E-12	16.999289	2
ATCGTTT	270	0.0	16.591898	29
GGACGTG	195	0.0	16.412207	6
CCTATTC	265	0.0	16.302076	3
CATCGTT	275	0.0	16.290228	28
GTATAGA	120	3.101013E-8	16.002758	1
GTCGCGT	120	3.103014E-8	16.001902	8
ACACGCT	280	0.0	16.001902	9
ACCGTCG	170	3.6379788E-12	16.001902	8
CGCGTAA	110	2.0164771E-7	16.001043	10
TAGGACG	180	0.0	16.000189	4
AACGAAC	130	4.798494E-9	15.9993305	14
CGAGCCG	295	0.0	15.728156	15
>>END_MODULE