FastQCFastQC Report
Thu 2 Feb 2017
SRR4063003_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063003_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2142591
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT107070.49972206548053266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT69970.32656722631617513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT40280.18799668252130247No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG34220.1597131697090112No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA34060.15896641029482528No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG31470.1468782422776909No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31430.14669155242414442No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC29970.139877372769698No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG29110.13586354091844874No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA29040.1355368336747424No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG28330.13222308877429242No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG27110.12652904824112488No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC26650.12438211492534039No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA26600.12414875260840728No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA26410.12326197580406152No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT26180.12218850914616929No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26160.12209516421939604No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT26000.12134840480521016No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA25210.11766128019766722No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG24610.11486093239447005No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23980.11192056720111304No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT22880.10678659622858491No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC22700.10594649188762578No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT22470.10487302522973352No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT22300.104079593352161No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC22160.10342617886474834No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA550.001356627917.4708961
ATCGTTT7050.016.34007829
CTAGCGG6650.016.11995929
CCGTCTA703.6988553E-415.9996629
TAGTACT1102.0268817E-715.9963024
TCTAGCG6900.015.76741328
CATCGTT7400.015.56687528
ACTATAC1551.891749E-1015.4820983
TCGTTTA7450.015.24830
TAGCGGC7100.015.09827230
CGTTTAT7600.014.94705231
ACCGGTC650.004165017514.7682298
AACGCTT7550.014.41029230
AAACGCT7700.014.33735929
GATATAC3950.014.1904961
ATAGCGT5300.014.1860616
GGCATCG8400.014.0949426
GTATAAT2500.014.0931881
GCATCGT8300.014.07133527
CGGCATC8500.013.92944125
Produced by FastQC (version 0.11.5)