FastQCFastQC Report
Thu 2 Feb 2017
SRR4063003_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063003_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2142591
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT129760.6056218849047719No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT92980.43396056456878607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT55580.25940555150283No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38950.1817892448908821No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG37510.17506841016320895No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA32850.15331904222504433No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC32590.15210555817699226No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA31520.147111604594624No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA30590.1427710654996684No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG30240.14113752928113674No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC28660.13376328006605087No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG28320.13217641631090582No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG28210.131663019213653No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC28200.1316163467502664No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT26970.12587563375371222No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG26750.12484883955920659No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC25910.12092835263473056No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT25610.11952817873313198No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT25600.11948150626974537No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA25340.11826802222169328No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC24550.11458089761415034No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG24340.11360077588303133No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT24010.1120605845912729No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT23810.11112713532354052No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC22970.1072066483990645No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG22410.10459299044941381No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA21960.10249272959701596No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG21680.10118590062219061No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT21660.10109255569541738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG7150.017.45425229
TAGCGGC7350.016.97930730
TCTAGCG7750.016.51585228
GTACTAC908.585295E-616.0004791
ATCGTTT5000.015.99973129
CATCGTT5450.015.55937228
ACCGTCG6200.015.2266178
TACCGTC6400.015.0007987
ATACCGT6600.014.7886656
GTATAGA1654.8567017E-1014.5458881
CGCCCCT5400.014.5192915
GATATAC3200.014.5004331
GCATCGT6000.014.39975827
AGCGGCG8900.014.38178131
GCCCCTT6250.014.3367636
CGTCGTA6700.014.08964510
TCCCCGC5550.013.838251
TCGTTTA5700.013.75415630
GTCGTAG7100.013.74624811
TAATACC2107.2759576E-1213.7150154
Produced by FastQC (version 0.11.5)