##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063003_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2142591
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.126911295716262	32.0	32.0	32.0	32.0	32.0
2	31.296533496126887	32.0	32.0	32.0	32.0	32.0
3	31.375591515132847	32.0	32.0	32.0	32.0	32.0
4	31.4811538926468	32.0	32.0	32.0	32.0	32.0
5	31.402854767895505	32.0	32.0	32.0	32.0	32.0
6	34.912650151148775	36.0	36.0	36.0	36.0	36.0
7	34.94108488274244	36.0	36.0	36.0	36.0	36.0
8	34.891391777525435	36.0	36.0	36.0	32.0	36.0
9	34.97817548939578	36.0	36.0	36.0	36.0	36.0
10	34.83865749459416	36.0	36.0	36.0	32.0	36.0
11	35.0168450254855	36.0	36.0	36.0	36.0	36.0
12	34.90230006566816	36.0	36.0	36.0	32.0	36.0
13	34.954651167675024	36.0	36.0	36.0	36.0	36.0
14	34.89729817776701	36.0	36.0	36.0	32.0	36.0
15	34.87663674495039	36.0	36.0	36.0	32.0	36.0
16	34.878177402966784	36.0	36.0	36.0	32.0	36.0
17	34.83877230885409	36.0	36.0	36.0	32.0	36.0
18	34.83825004398879	36.0	36.0	36.0	32.0	36.0
19	34.82868498934234	36.0	36.0	36.0	32.0	36.0
20	34.805945231731116	36.0	36.0	36.0	32.0	36.0
21	34.7988958228612	36.0	36.0	36.0	32.0	36.0
22	34.785459287376824	36.0	36.0	36.0	32.0	36.0
23	34.72681020316057	36.0	36.0	36.0	32.0	36.0
24	34.69928791822611	36.0	36.0	36.0	32.0	36.0
25	34.67793713312527	36.0	36.0	36.0	32.0	36.0
26	34.61562146018535	36.0	36.0	36.0	32.0	36.0
27	34.602093913397376	36.0	36.0	36.0	32.0	36.0
28	34.565995096590996	36.0	36.0	36.0	32.0	36.0
29	34.52796824032212	36.0	36.0	36.0	32.0	36.0
30	34.498377898535	36.0	36.0	36.0	32.0	36.0
31	34.49163372757563	36.0	36.0	36.0	32.0	36.0
32	34.45832779097831	36.0	36.0	36.0	32.0	36.0
33	34.405704121785256	36.0	36.0	36.0	32.0	36.0
34	34.38439487517683	36.0	36.0	36.0	32.0	36.0
35	34.34634234905309	36.0	36.0	36.0	32.0	36.0
36	34.31232792446155	36.0	36.0	36.0	32.0	36.0
37	34.29992751766436	36.0	36.0	36.0	32.0	36.0
38	33.8596222984228	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	8.0
20	16.0
21	73.0
22	214.0
23	626.0
24	1659.0
25	3914.0
26	8011.0
27	15567.0
28	26596.0
29	41818.0
30	61796.0
31	88334.0
32	125107.0
33	199156.0
34	478357.0
35	1091338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.73899148108507	18.847521645026696	12.626580638897615	25.78690623499061
2	15.475982116978928	21.47544725054852	36.87628670147499	26.172283930997565
3	17.37475794493676	26.138119687798557	29.08996630714868	27.397156060116
4	11.869430800508544	16.72239366597778	36.222736242338705	35.18543929117497
5	14.008926575347324	37.22268972473048	32.70026803995723	16.068115659964967
6	32.922895115626524	36.54234971912472	16.974612011268615	13.560143153980139
7	28.900817377903216	31.396356640559546	21.5006839875048	18.202141994032438
8	28.185375580039633	32.041804860244405	19.898381629907874	19.874437929808085
9	28.115093367386944	13.8796940402445	18.833897159585234	39.171315432783324
10	16.34662281903377	26.43011596815228	30.663114547895965	26.560146664917987
11	37.09184699837152	21.505474712331978	22.30795332713804	19.094724962158462
12	25.158523391826513	24.230952689880358	28.305094871337722	22.305429046955407
13	29.46730234739812	19.56253777563084	25.484963257000302	25.485196619970736
14	24.393129626699636	19.97502089759548	25.277619480339457	30.354229995365422
15	25.29470160193896	26.499037847167283	23.179785596037693	25.02647495485606
16	25.594981396330045	25.73817271449294	23.86819117814531	24.798654711031705
17	23.76202457678577	25.569322376505827	25.460482191888232	25.208170854820168
18	24.970934723425984	24.087098284273573	27.01164151254252	23.93032547975792
19	25.543885883960122	24.713862795092485	26.253400672363508	23.488850648583888
20	25.933554280775006	23.41454808687239	25.7358030534059	24.916094578946705
21	27.633391191684453	23.69250472209089	24.886574139977384	23.787529946247275
22	26.0179847670414	23.66345233411323	25.50099389010782	24.817569008737554
23	23.970263998491546	23.395631824690515	26.36181104346706	26.272293133350882
24	24.561570547061944	25.034455946095175	25.811039064385128	24.592934442457754
25	24.83503610116728	23.960253711629385	26.035499092220167	25.169211094983172
26	24.44883269726485	24.962556981296927	26.618310837962856	23.970299483475365
27	25.382037627357544	24.29400865307875	25.43099706430068	24.892956655263024
28	24.07580354813401	24.258432897365854	26.686381115201176	24.979382439298963
29	24.174469135733325	24.476066594137656	26.48354258932293	24.86592168080609
30	24.28246921600996	24.6248584074142	26.788220430310776	24.30445194626506
31	24.743033084709122	24.56759129483882	25.28037315567927	25.40900246477279
32	24.43821522633111	24.59722830908932	25.192908959292744	25.771647505286822
33	24.0266574441879	24.00929528780808	26.192913159814452	25.77113410818957
34	24.874602758996	24.287556514519103	26.361260735249985	24.47657999123491
35	25.940275115502683	24.092045565392556	26.082812818685415	23.884866500419353
36	24.517838448868684	25.09013619491541	25.45926870784018	24.93275664837573
37	25.5404787941329	24.98036256103008	25.35378894058642	24.125369704250602
38	24.37607025892507	24.6332357722363	26.031264045507562	24.959429923331072
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	858.0
1	692.0
2	526.0
3	526.0
4	1692.5
5	2859.0
6	2859.0
7	3432.5
8	4006.0
9	3852.0
10	3698.0
11	3698.0
12	4681.5
13	5665.0
14	7084.0
15	8503.0
16	8503.0
17	12963.0
18	17423.0
19	17423.0
20	19487.0
21	21551.0
22	20582.5
23	19614.0
24	19614.0
25	20852.0
26	22090.0
27	22090.0
28	27742.0
29	33394.0
30	40724.0
31	48054.0
32	48054.0
33	60494.5
34	72935.0
35	72935.0
36	79295.0
37	85655.0
38	99076.0
39	112497.0
40	112497.0
41	120289.0
42	128081.0
43	146680.0
44	165279.0
45	165279.0
46	170448.5
47	175618.0
48	175618.0
49	186880.5
50	198143.0
51	196968.0
52	195793.0
53	195793.0
54	186621.5
55	177450.0
56	177450.0
57	174529.0
58	171608.0
59	152911.0
60	134214.0
61	134214.0
62	129629.0
63	125044.0
64	102250.5
65	79457.0
66	79457.0
67	66280.0
68	53103.0
69	53103.0
70	42777.5
71	32452.0
72	25101.5
73	17751.0
74	17751.0
75	13070.0
76	8389.0
77	8389.0
78	8684.5
79	8980.0
80	6899.0
81	4818.0
82	4818.0
83	4768.0
84	4718.0
85	4718.0
86	2947.0
87	1176.0
88	1033.0
89	890.0
90	890.0
91	522.0
92	154.0
93	112.5
94	71.0
95	71.0
96	56.0
97	41.0
98	41.0
99	37.0
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00849438833636471
2	0.0
3	0.0
4	1.4001739015985786E-4
5	0.0
6	1.4001739015985786E-4
7	3.7337970709295425E-4
8	0.003033710120130253
9	0.004060504314635878
10	9.334492677323857E-4
11	0.004853936192208406
12	2.3336231693309642E-4
13	2.800347803197157E-4
14	0.0
15	0.0
16	1.4001739015985786E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	9.334492677323856E-5
22	0.0
23	1.4001739015985786E-4
24	0.0
25	4.667246338661928E-5
26	9.334492677323856E-5
27	4.667246338661928E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	9.334492677323856E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2142591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.051724609382354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.75602220356029	29.86760187174665
2	15.044691160411663	12.352210367008572
3	5.209030234915287	6.415190240570658
4	2.3030863604028355	3.781826680755277
5	1.2762012612715734	2.619513136193353
6	0.7546981003604505	1.8588995147532734
7	0.4844636479378681	1.392164778088149
8	0.3394709870522697	1.1148695578675982
9	0.25508304826859607	0.9424439145039774
>10	1.3162076808337062	9.70363796007023
>50	0.10725461805329772	3.1177827244147363
>100	0.11903212638146811	10.58462142439697
>500	0.02149543178800451	6.020529215813437
>1k	0.012920126553428243	8.922344785751411
>5k	2.2867480625536713E-4	0.6973031413389604
>10k+	1.1433740312768356E-4	0.6090606867268679
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	12976	0.6056218849047719	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	9298	0.43396056456878607	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	5558	0.25940555150283	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3895	0.1817892448908821	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3751	0.17506841016320895	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3285	0.15331904222504433	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3259	0.15210555817699226	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	3152	0.147111604594624	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3059	0.1427710654996684	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3024	0.14113752928113674	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2866	0.13376328006605087	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2832	0.13217641631090582	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2821	0.131663019213653	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2820	0.1316163467502664	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2697	0.12587563375371222	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2675	0.12484883955920659	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2591	0.12092835263473056	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2561	0.11952817873313198	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2560	0.11948150626974537	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2534	0.11826802222169328	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2455	0.11458089761415034	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2434	0.11360077588303133	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	2401	0.1120605845912729	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	2381	0.11112713532354052	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	2297	0.1072066483990645	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	2241	0.10459299044941381	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2196	0.10249272959701596	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	2168	0.10118590062219061	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	2166	0.10109255569541738	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	4.667246338661929E-5	4.667246338661929E-5
6	0.0	0.0	0.0	4.667246338661929E-5	4.667246338661929E-5
7	0.0	0.0	0.0	4.667246338661929E-5	4.667246338661929E-5
8	0.0	0.0	0.0	4.667246338661929E-5	4.667246338661929E-5
9	0.0	0.0	0.0	4.667246338661929E-5	4.667246338661929E-5
10	0.0	0.0	0.0	4.667246338661929E-5	4.667246338661929E-5
11	0.0	0.0	0.0	4.667246338661929E-5	4.667246338661929E-5
12	0.0	0.0	0.0	4.667246338661929E-5	4.667246338661929E-5
13	0.0	0.0	0.0	4.667246338661929E-5	4.667246338661929E-5
14	0.0	0.0	0.0	4.667246338661929E-5	4.667246338661929E-5
15	0.0	0.0	0.0	4.667246338661929E-5	4.667246338661929E-5
16	0.0	0.0	0.0	4.667246338661929E-5	4.667246338661929E-5
17	0.0	0.0	0.0	4.667246338661929E-5	4.667246338661929E-5
18	0.0	0.0	0.0	4.667246338661929E-5	4.667246338661929E-5
19	0.0	0.0	0.0	9.334492677323858E-5	4.667246338661929E-5
20	0.0	0.0	0.0	1.8668985354647715E-4	4.667246338661929E-5
21	0.0	0.0	0.0	4.6672463386619284E-4	9.334492677323858E-5
22	0.0	0.0	0.0	9.334492677323857E-4	9.334492677323858E-5
23	0.0	0.0	0.0	0.0020535883890112484	9.334492677323858E-5
24	0.0	0.0	0.0	0.0037337970709295427	9.334492677323858E-5
25	0.0	0.0	0.0	0.004853936192208406	9.334492677323858E-5
26	0.0	0.0	0.0	0.00583405792332741	1.4001739015985786E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	715	0.0	17.454252	29
TAGCGGC	735	0.0	16.979307	30
TCTAGCG	775	0.0	16.515852	28
GTACTAC	90	8.585295E-6	16.000479	1
ATCGTTT	500	0.0	15.999731	29
CATCGTT	545	0.0	15.559372	28
ACCGTCG	620	0.0	15.226617	8
TACCGTC	640	0.0	15.000798	7
ATACCGT	660	0.0	14.788665	6
GTATAGA	165	4.8567017E-10	14.545888	1
CGCCCCT	540	0.0	14.519291	5
GATATAC	320	0.0	14.500433	1
GCATCGT	600	0.0	14.399758	27
AGCGGCG	890	0.0	14.381781	31
GCCCCTT	625	0.0	14.336763	6
CGTCGTA	670	0.0	14.089645	10
TCCCCGC	555	0.0	13.83825	1
TCGTTTA	570	0.0	13.754156	30
GTCGTAG	710	0.0	13.746248	11
TAATACC	210	7.2759576E-12	13.715015	4
>>END_MODULE