FastQCFastQC Report
Thu 2 Feb 2017
SRR4063002_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063002_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335623
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30030.22483889540686258No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20470.15326181115479443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT20280.1518392540409981No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20120.1506413112083275No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20100.15049156835424368No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18340.13731419719486712No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18080.1353675400917774No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17730.13274704014531047No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17690.13244755443714282No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17380.13012654019884354No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16650.12466092602478394No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16500.12353785461915526No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16270.12181581179719128No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG15370.11507738336341918No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT14700.11006099775161104No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC14360.10751536923218603No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT14270.10684152638880881No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT14050.10519435499388675No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA14030.10504461213980293No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13950.10444564072346764No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC13900.10407128358825807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAC350.002067023222.85655611
TCTAGCG4500.018.48841328
CTAGCGG4550.018.28524429
CTTACAC1453.6379788E-1217.6553783
TAGCGGC5000.016.63957230
ATCGTTT3850.016.62294829
GTATTAT1407.3305273E-1016.0151791
AACGAAT1102.0204789E-715.99958831
CATCGTT4050.015.80206328
CGTTTAT4000.015.59959831
GAACCGC2500.015.3578816
CGGTCCA6100.015.21272410
GTATAAT951.4199371E-515.1722751
TAGACCG756.251835E-414.9312724
GCATCGT4300.014.88333827
GGCATCG4100.014.82888626
CGCCGGT6050.014.8089827
ACTATAC650.00416172514.7694033
TATACCC650.004165869714.7671925
AGAACCG2750.014.54344755
Produced by FastQC (version 0.11.5)