##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063002_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1335623
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.235619632186626	32.0	32.0	32.0	32.0	32.0
2	30.827496980809705	32.0	32.0	32.0	32.0	32.0
3	30.879480212604903	32.0	32.0	32.0	32.0	32.0
4	30.94858504233605	32.0	32.0	32.0	32.0	32.0
5	30.840308979405116	32.0	32.0	32.0	32.0	32.0
6	34.50529752782035	36.0	36.0	36.0	32.0	36.0
7	34.425860441157425	36.0	36.0	36.0	32.0	36.0
8	34.41556561993916	36.0	36.0	36.0	32.0	36.0
9	34.563209079208725	36.0	36.0	36.0	32.0	36.0
10	34.26899431950483	36.0	36.0	36.0	32.0	36.0
11	34.543371145899705	36.0	36.0	36.0	32.0	36.0
12	34.34290364870925	36.0	36.0	36.0	32.0	36.0
13	34.43791548962544	36.0	36.0	36.0	32.0	36.0
14	34.337765222671365	36.0	36.0	36.0	32.0	36.0
15	34.272058058299386	36.0	36.0	36.0	32.0	36.0
16	34.29196337589275	36.0	36.0	36.0	32.0	36.0
17	34.21199619952636	36.0	36.0	36.0	32.0	36.0
18	34.24596686340382	36.0	36.0	36.0	32.0	36.0
19	34.23972558124561	36.0	36.0	36.0	32.0	36.0
20	34.22029644592823	36.0	36.0	36.0	32.0	36.0
21	34.20365252769681	36.0	36.0	36.0	32.0	36.0
22	34.17257714190307	36.0	36.0	36.0	32.0	36.0
23	34.1225697670675	36.0	36.0	36.0	32.0	36.0
24	34.09533154190966	36.0	36.0	36.0	32.0	36.0
25	34.069851297858754	36.0	36.0	36.0	32.0	36.0
26	34.02453162307028	36.0	36.0	36.0	32.0	36.0
27	34.035209785995	36.0	36.0	36.0	32.0	36.0
28	34.01413647413978	36.0	36.0	36.0	32.0	36.0
29	33.97568700149668	36.0	36.0	36.0	32.0	36.0
30	33.94401563914368	36.0	36.0	36.0	32.0	36.0
31	33.97681756004501	36.0	36.0	36.0	32.0	36.0
32	33.93302825722528	36.0	36.0	36.0	32.0	36.0
33	33.89292712090163	36.0	36.0	36.0	32.0	36.0
34	33.899256002629485	36.0	36.0	36.0	32.0	36.0
35	33.862694038662106	36.0	36.0	36.0	32.0	36.0
36	33.812155825408816	36.0	36.0	36.0	32.0	36.0
37	33.827814435660365	36.0	36.0	36.0	32.0	36.0
38	33.221254800194366	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	2.0
9	3.0
10	12.0
11	10.0
12	8.0
13	6.0
14	187.0
15	390.0
16	507.0
17	584.0
18	740.0
19	929.0
20	1179.0
21	1663.0
22	2638.0
23	4018.0
24	5970.0
25	9111.0
26	13330.0
27	18943.0
28	26815.0
29	35602.0
30	47988.0
31	64977.0
32	88798.0
33	133819.0
34	294394.0
35	582997.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.22365851738858	17.4747857113409	11.389100231248102	25.91245554002241
2	16.945373968191184	19.866687941461354	37.160923595769916	26.02701449457755
3	18.801180713220276	23.75181959229152	28.692724027004846	28.75427566748336
4	12.55248608856597	15.28408376210699	36.24325392784623	35.92017622148081
5	14.817751442961738	36.366304513031764	33.41906124566775	15.396882798338751
6	33.97358530120842	35.762716940447135	16.8639283628577	13.399769395486741
7	30.234527433660123	30.537457454279725	20.903219623516232	18.324795488543916
8	28.175699806606524	32.48935512298043	19.71857243719953	19.616372633213512
9	27.61828036288792	14.092566817333385	18.794506517202887	39.49464630257581
10	16.20132687540384	26.063046422669938	31.128637732766606	26.606988969159616
11	37.65268395824505	21.1408039221265	22.315854573443286	18.890657546185167
12	25.023435370788	23.75425655217028	28.314869555581513	22.90743852146021
13	29.366954388576588	19.018924947938235	25.404269626405817	26.209851037079357
14	23.81383110557343	20.061894560601782	24.676581078055584	31.4476932557692
15	25.28448215822263	27.255394021362655	22.105995487227414	25.354128333187305
16	26.016563333033815	25.567586186240156	23.66242849013089	24.753421990595143
17	24.20283907608712	25.811239482268505	25.083647604698044	24.902273836946332
18	25.322487413481724	24.272213220561326	26.272054485173218	24.13324488078373
19	25.77704698559456	24.77333087739239	25.323596426006915	24.126025711006136
20	26.026847486866778	24.012986468435336	24.73508922206981	25.225076822628083
21	27.51561719654943	24.01907233515556	24.058756449604545	24.406554018690468
22	26.13912302521185	24.17345114984916	24.74371463464126	24.943711190297734
23	24.2266883316236	23.878052250484842	25.597421171255498	26.29783824663607
24	24.850760797939255	24.947882345893486	25.196040256394898	25.00531659977236
25	25.01104526386574	24.280635068974867	25.232624489484163	25.475695177675238
26	24.579466476759173	25.441913093357954	25.704308938158004	24.27431149172487
27	25.504230625233994	24.58105578435043	24.662972669412206	25.251740921003368
28	24.48624162330126	24.570850960278538	25.714200142263486	25.228707274156715
29	24.474708666511418	24.828210022186497	25.57414954881965	25.12293176248243
30	24.237076459321976	25.13975139002655	25.907878639039346	24.71529351161213
31	25.311950833628465	24.78295410045472	24.42489856216217	25.480196503754645
32	24.73349591511404	24.761349962898706	24.41017930668879	26.09497481529846
33	24.285108849150667	24.372266042090036	25.367759925362293	25.974865183397007
34	25.33943157005634	24.466928447555464	25.476312423809482	24.717327558578717
35	26.07886261233433	24.29544162808304	25.40656022752546	24.219135532057173
36	24.54569280536752	25.155753908824057	24.858548493380457	25.440004792427963
37	25.794833395731935	24.912691875701984	24.698539872706853	24.593934855859228
38	24.58227010254936	24.72655501245927	25.368536754839948	25.322638130151425
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	523.0
1	409.5
2	296.0
3	296.0
4	600.0
5	904.0
6	904.0
7	1016.5
8	1129.0
9	1082.5
10	1036.0
11	1036.0
12	1357.0
13	1678.0
14	2249.5
15	2821.0
16	2821.0
17	4123.5
18	5426.0
19	5426.0
20	6644.5
21	7863.0
22	8760.0
23	9657.0
24	9657.0
25	11591.0
26	13525.0
27	13525.0
28	17242.0
29	20959.0
30	25606.0
31	30253.0
32	30253.0
33	39361.0
34	48469.0
35	48469.0
36	53280.5
37	58092.0
38	65932.5
39	73773.0
40	73773.0
41	78036.5
42	82300.0
43	92663.0
44	103026.0
45	103026.0
46	104612.0
47	106198.0
48	106198.0
49	113694.5
50	121191.0
51	123249.5
52	125308.0
53	125308.0
54	118541.5
55	111775.0
56	111775.0
57	108663.5
58	105552.0
59	94357.5
60	83163.0
61	83163.0
62	80423.5
63	77684.0
64	64161.5
65	50639.0
66	50639.0
67	42637.5
68	34636.0
69	34636.0
70	28125.0
71	21614.0
72	16927.0
73	12240.0
74	12240.0
75	9275.0
76	6310.0
77	6310.0
78	6286.0
79	6262.0
80	4957.5
81	3653.0
82	3653.0
83	3742.5
84	3832.0
85	3832.0
86	2560.0
87	1288.0
88	1114.0
89	940.0
90	940.0
91	664.5
92	389.0
93	355.5
94	322.0
95	322.0
96	287.0
97	252.0
98	252.0
99	448.5
100	645.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11664968333129933
2	0.05315871319975772
3	0.011904556899664052
4	0.0023210142382992806
5	1.4974285408382454E-4
6	2.2461428112573683E-4
7	7.487142704191227E-5
8	2.994857081676491E-4
9	2.994857081676491E-4
10	2.994857081676491E-4
11	0.0015722999678801578
12	0.0026204999464669297
13	0.014225571137963333
14	0.009433799807280947
15	0.022611170966657507
16	0.010706614066993456
17	0.01939169960385528
18	0.005016385611808122
19	0.006438942725604456
20	0.005914842736311069
21	0.005690228455185333
22	0.0074871427041912275
23	0.009957899796574333
24	0.013701471148669948
25	0.015648128251759666
26	0.017669656781891297
27	0.00920918552615521
28	0.0054656141740595954
29	0.009433799807280947
30	0.002770242800550754
31	0.005690228455185333
32	0.0067384284337721045
33	0.00786149983940079
34	0.011605071191496404
35	0.015872742532885405
36	0.013701471148669948
37	0.00920918552615521
38	0.005315871319975771
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1335623.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.2145780099482
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.11967018935738	45.89417513831439
2	10.689734928389981	11.804584062185134
3	2.717382995450189	4.501174661555735
4	1.064510274498768	2.3510594237481435
5	0.6126580500045367	1.6913827847699119
6	0.3725752122927785	1.234294987422758
7	0.24345287551124445	0.940950345066327
8	0.17635075060701372	0.7789705821203103
9	0.12335958255250856	0.6130122564708116
>10	0.6965100316642179	7.114848515959617
>50	0.07551820312251895	2.9673144397552567
>100	0.08989027592474294	10.880800647780367
>500	0.013074937332759402	4.912413434635448
>1k	0.005311693291433507	4.315018720215878
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3003	0.22483889540686258	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2047	0.15326181115479443	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2028	0.1518392540409981	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2012	0.1506413112083275	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2010	0.15049156835424368	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1834	0.13731419719486712	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1808	0.1353675400917774	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1773	0.13274704014531047	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1769	0.13244755443714282	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1738	0.13012654019884354	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1665	0.12466092602478394	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1650	0.12353785461915526	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1627	0.12181581179719128	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1537	0.11507738336341918	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1470	0.11006099775161104	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1436	0.10751536923218603	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1427	0.10684152638880881	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1405	0.10519435499388675	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1403	0.10504461213980293	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1395	0.10444564072346764	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1390	0.10407128358825807	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.487142704191227E-5
2	0.0	0.0	0.0	0.0	7.487142704191227E-5
3	0.0	0.0	0.0	0.0	7.487142704191227E-5
4	0.0	0.0	0.0	0.0	7.487142704191227E-5
5	7.487142704191227E-5	0.0	0.0	0.0	7.487142704191227E-5
6	7.487142704191227E-5	0.0	0.0	0.0	7.487142704191227E-5
7	7.487142704191227E-5	0.0	0.0	0.0	7.487142704191227E-5
8	7.487142704191227E-5	0.0	0.0	7.487142704191227E-5	7.487142704191227E-5
9	7.487142704191227E-5	0.0	0.0	7.487142704191227E-5	7.487142704191227E-5
10	7.487142704191227E-5	0.0	0.0	7.487142704191227E-5	7.487142704191227E-5
11	7.487142704191227E-5	0.0	0.0	7.487142704191227E-5	7.487142704191227E-5
12	7.487142704191227E-5	0.0	0.0	7.487142704191227E-5	1.4974285408382454E-4
13	7.487142704191227E-5	0.0	0.0	7.487142704191227E-5	2.994857081676491E-4
14	7.487142704191227E-5	0.0	0.0	7.487142704191227E-5	2.994857081676491E-4
15	7.487142704191227E-5	0.0	0.0	7.487142704191227E-5	2.994857081676491E-4
16	7.487142704191227E-5	0.0	0.0	7.487142704191227E-5	5.240999892933859E-4
17	7.487142704191227E-5	0.0	0.0	1.4974285408382454E-4	5.240999892933859E-4
18	7.487142704191227E-5	0.0	0.0	4.4922856225147365E-4	5.989714163352982E-4
19	7.487142704191227E-5	0.0	0.0	5.240999892933859E-4	5.989714163352982E-4
20	7.487142704191227E-5	0.0	0.0	5.240999892933859E-4	5.989714163352982E-4
21	7.487142704191227E-5	0.0	0.0	5.240999892933859E-4	6.738428433772105E-4
22	7.487142704191227E-5	0.0	0.0	8.23585697461035E-4	6.738428433772105E-4
23	7.487142704191227E-5	0.0	0.0	0.0014225571137963333	6.738428433772105E-4
24	7.487142704191227E-5	0.0	0.0	0.002171271384215456	6.738428433772105E-4
25	7.487142704191227E-5	0.0	0.0	0.002470757092383105	6.738428433772105E-4
26	7.487142704191227E-5	0.0	0.0	0.0037435713520956137	6.738428433772105E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCGAC	35	0.0020670232	22.856556	11
TCTAGCG	450	0.0	18.488413	28
CTAGCGG	455	0.0	18.285244	29
CTTACAC	145	3.6379788E-12	17.655378	3
TAGCGGC	500	0.0	16.639572	30
ATCGTTT	385	0.0	16.622948	29
GTATTAT	140	7.3305273E-10	16.015179	1
AACGAAT	110	2.0204789E-7	15.999588	31
CATCGTT	405	0.0	15.802063	28
CGTTTAT	400	0.0	15.599598	31
GAACCGC	250	0.0	15.357881	6
CGGTCCA	610	0.0	15.212724	10
GTATAAT	95	1.4199371E-5	15.172275	1
TAGACCG	75	6.251835E-4	14.931272	4
GCATCGT	430	0.0	14.883338	27
GGCATCG	410	0.0	14.828886	26
CGCCGGT	605	0.0	14.808982	7
ACTATAC	65	0.004161725	14.769403	3
TATACCC	65	0.0041658697	14.767192	5
AGAACCG	275	0.0	14.5434475	5
>>END_MODULE