FastQCFastQC Report
Thu 2 Feb 2017
SRR4063002_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063002_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335623
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35110.262873580344154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25510.19099701038391823No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21260.1591766538911055No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21230.15895203960997975No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19560.14644851129398043No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19440.14555005416947747No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19060.1427049399418848No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18900.1415069971092142No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18540.13881162573570535No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18250.1366403543514899No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA17420.1304260259070112No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17110.1281050116687119No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16250.12166606894310746No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16020.11994402612114347No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15370.11507738336341918No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG15180.11365482624962284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14940.11185791200061694No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14800.11080971202203017No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT14170.1060928121183897No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC13750.10294821218262938No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT13580.10167539792291687No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT13510.1011512979336235No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT13390.10025284080912053No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA308.4161997E-426.6674169
ATACCGA400.004484096519.99981528
TCTAGCG3850.018.70112228
GTAGGAC3900.018.4613673
TAGGACG2000.017.5998364
CTAGCGG4100.017.5608129
TGTAGGA4500.017.4220582
TAGCGGC4350.017.28719330
TCTAGAT4150.016.1926212
GTTATAT1153.3448669E-715.3047781
TAAACGC3500.015.08557328
GTATCAA25900.015.0737821
TAGATAA4550.014.7690934
ATCGTTT3800.014.73670529
TACCGTC3400.014.5886447
CGGCATC3950.014.58214225
ACGTTTT1654.838512E-1014.54531929
CGCTTCG3550.014.422432
CATCGTT3900.014.35884128
CGACCCG3150.014.2226215
Produced by FastQC (version 0.11.5)