FastQCFastQC Report
Thu 2 Feb 2017
SRR4063001_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063001_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160897
Sequences flagged as poor quality0
Sequence length38
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT62613.8913093469735296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT43082.677489325469089No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA26441.643287320459673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT22841.4195416943759052No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20041.2455173185329744No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA14590.9067913012672704No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13590.8446397384662238No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA12730.7911893944573237No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA11680.7259302535162246No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10680.663778690715178No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA9480.589196815353922No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7660.4760809710560172No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT7220.4487342834235567No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6600.41020031448690775No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA6550.4070927363468554No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG5770.3586145173620391No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA4970.3088932671212018No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4860.3020565952130866No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA4750.2952199233049715No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.25917201688036445No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA4160.258550501252354No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA4070.2529568606002598No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA3840.23866200115601907No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA3780.2349329073879563No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA3460.21504440729162136No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3290.2044786416154434No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA3050.18956226654319222No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG2980.18521165714711896No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA2860.17775346961099336No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA2660.16532315705078404No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA2650.16470164142277358No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2560.15910800077067938No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC2540.15786496951465845No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2530.15724345388664798No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2520.15662193825863752No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2520.15662193825863752No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA2500.15537890700261658No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA2360.14667768821047006No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2360.14667768821047006No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2350.1460561725824596No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2310.14357011007041773No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC2130.13238282876622934No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA1950.12119554746204093No Hit
GTACATGGGTGTGGTATCAACGCAAAAAAAAAAAAAAA1920.11933100057800954No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT1860.11560190680994674No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG1840.11435887555392579No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT1830.11373735992591533No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA1800.11187281304188394No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1790.11125129741387348No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1780.110629781785863No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAA1730.10752220364581068No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1720.1069006880178002No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT1720.1069006880178002No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG1690.10503614113376882No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA1660.1031715942497374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCCA200.003744715831.99875613
GGTCTAC308.3567534E-426.6822191
CTGCTTA308.3871826E-426.6656325
TGGGTAG400.004468029419.9992246
TAGGACC400.004468029419.9992244
ATGGGTA601.1308086E-418.6659415
GGAGAGT450.00882012917.7770868
GCCGCCC450.00882012917.77708612
AAGTACT652.0819539E-417.2354582
TACATAG600.002425260616.0043542
AGTACTC703.6641644E-416.0043533
TGGGAGA650.00414199914.7686586
CCTTGGC800.00100919614.003812
CATGGGT5750.012.7995024
TGGGTGG4050.012.6414856
CTTCCAT3850.012.46704928
GCTTCCA3850.012.46704927
TTCCATG4000.012.39951929
ATGGAAG5300.012.3768795
ATGGGTG4700.012.2548435
Produced by FastQC (version 0.11.5)